1887

Abstract

DNA complementary to mosaic virus (NVMV) RNA was qloned and five segments larger than 0·9 kb were used in Northern blot hybridization analysis to identify two virus-specific RNAs, approximately 8 kb (RNA 1) and 3 kb (RNA 2) in size. The clones selected as probes did not hybridize with RNA from various tobamoviruses, or from beet necrotic yellow vein (BNYVV) and peanut clump furoviruses. In an attempt to determine the taxonomic position of the virus, about 75 % of the NVMV RNA 2 was sequenced and four open reading frames (ORFs) were identified. ORFs 1,2 and 3 encode proteins of 20K, 39K and 13K, whereas ORF 4 was incomplete. ORFs 2, 3 and 4 overlapped in an arrangement closely resembling the triple gene block identified in BNYVV RNA 2, barley stripe mosaic virus (BSMV) RNA 2, potato virus × and potato virus M RNA. The presumed coat protein gene of NVMV RNA 2 (ORF 1) is situated to the 5′ side of the triple gene block as for BNYVV and BSMV RNA 2. Amino acid homologies were detected among the 13K and 14K proteins of NVMV RNA 2, BNYVV RNA 2 and BSMV RNA 2. Significant homology was also detected between the 39K protein of NVMV RNA 2 and the 42K protein of BNYVV RNA 2, with a motif specific for ATP- and GTP-binding (NTP-binding motif), and a conserved viral DNA polymerase domain. The presence of a triple gene block in NVMV RNA 2 indicates that NVMV has affinities with members of the hordei-, furo-, potex- and carlavirus groups but not with the tobamovirus group. The divided RNA genome of NVMV, and the sizes of the two RNAs suggest that NVMV is most closely allied to the furoviruses, but the unique nature of its different biological properties and lack of any serological relationships with furoviruses lead us to conclude that NVMV has no clear relatedness to any taxonomic group of plant viruses.

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1990-05-01
2024-11-03
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References

  1. Batista M. F., Antoniw J. F., Swaby A. G., Jones P., Adams M. J. 1989; RNA/cDNA hybridization studies of UK isolates of barley yellow mosaic virus. Plant Pathology 38:226–229
    [Google Scholar]
  2. Bouzoubaa S., Quillet L., Guilley H., Jonard G., Richards K. 1987; Nucleotide sequence of beet necrotic yellow vein virus RNA-1. Journal of General Virology 68:615–626
    [Google Scholar]
  3. Brown F. 1989; The classification and nomenclature of viruses: summary of results of meetings of the International Committee on Taxonomy of Viruses in Edmonton, Canada, 1987. Intervirology 30:181–186
    [Google Scholar]
  4. Brunt A. A., Richards K. E. 1989; Biology and molecular biology of furoviruses. Advances in Virus Research 36:1–32
    [Google Scholar]
  5. Brunt A. A., Shikata E. 1986; Fungus-transmitted and similar labile rod-shaped viruses. In The Plant Viruses, 2 pp 305–335 Van Regenmortel M. H. V., Fraenkel-Conrat H. Edited by New York: Plenum Press;
    [Google Scholar]
  6. Burgermeister W., Koenig R., Weich H., Sebald W., Lesemann D.-E. 1986; Diversity of the RNAs in thirteen isolates of beet necrotic yellow vein virus in Chenopodium quinoadetected by means of cloned cDNAs. Journal of Phytopathology 115:229–242
    [Google Scholar]
  7. Dougherty W. G., Hiebert E. 1985; Genome structure and gene expression of plant RNA viruses. In Molecular Plant Virology 2 pp 23–81 Davies J. W. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  8. Feinberg A. P., Vogelstein B. 1984; A technique for labeling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 137:266–267
    [Google Scholar]
  9. Francki R.I.B., Hu J., Palukaitis P. 1986; Taxonomy of cucurbit infecting tobamoviruses as determined by serological and molecular hybridization analysis. Intervirology 26:156–163
    [Google Scholar]
  10. Hodgman T. C. 1986; An amino acid sequence motif linking viral DNA polymerases and plant virus proteins involved in RNA replication. Nucleic Acids Research 14:6769
    [Google Scholar]
  11. Hodgman T. C. 1988; A new superfamily of replicative proteins. Nature; London: 33322–23
    [Google Scholar]
  12. Koenig R., Burgermeister W. 1989; Mechanical inoculation of sugarbeet roots with isolates of beet necrotic yellow vein virus having different RNA compositions. Journal of Phytopathology 124:249–255
    [Google Scholar]
  13. Koenig R., Hum W. 1988; RNA/cDNA hybridization and infectivity tests suggest that barley yellow mosaic virus isolate M has a bipartite genome. Journal of Phytopathology 121:379–382
    [Google Scholar]
  14. Lemaire O., Merdinoglu D., Valentin P., Putz C., Ziegler-Graff V., Guilley H., Jonard G., Richards K. 1988; Effect of beet necrotic yellow vein virus RNA composition on transmission by Polymyxa betae . Virology 162:232–235
    [Google Scholar]
  15. Matthews R. E. F. 1982; Classification and nomenclature of viruses. Intervirology 17:1–199
    [Google Scholar]
  16. Maxam A. M., Gilbert W. 1980; Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–560
    [Google Scholar]
  17. Morozov S. YU., Dolja V. V., Atabekov J. G. 1989; Probable reassortment of genomic elements among elongated RNA-contain- ing plant viruses. Journal of Molecular Evolution 29:52–62
    [Google Scholar]
  18. Morozov S. YU., Lukasheva L. I., Chernov B. K., Skryabin K. G., Atabekov J. G. 1987; Nucleotide sequence of the open reading frames adjacent to the coat protein cistron in potato virus × genome. FEBS Letters 213:438–442
    [Google Scholar]
  19. Randles J. W. 1978; Nicotiana velutinaMOSAIC VIRU. CMIjAAB Descriptions of Plant Viruses189
    [Google Scholar]
  20. Randles J. W., Harrison B. D., Roberts I. M. 1976; Nicotiana velutinamosaic virus: purification, properties and affinities with other rod-shaped viruses. Annals of Applied Biology 84:193–204
    [Google Scholar]
  21. Rupasov V. V., Morozov S. YU., Kanyuka K. V., Zavriev S. K. 1989; Partial nucleotide sequence of potato virus M RNA shows similarities to potexviruses in gene arrangement and the encoded amino acid sequences. Journal of General Virology 70:1861–1869
    [Google Scholar]
  22. Schwarz-Sommer ZS., Shepherd N., Tacke E., Glerl A., Rohde W., Leclercq L., Mattes M., Berndtgen R., Peterson P. A., Saedler H. 1985; Influence of transposable elements on the structure and function of the A1 gene of Zea mays . EMBO Journal 6:287–294
    [Google Scholar]
  23. Steven A. C., Trus B. L., Putz C., Wurtz M. 1981; The molecular organization of beet necrotic yellow vein virus. Virology 113:428–438
    [Google Scholar]
  24. Walker J. E., Saraste M., Runswick M. J., Gay N. J. 1982; Distantly related sequences in the α-and β-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO Journal 1:945–951
    [Google Scholar]
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