Demonstration of enzyme activities required for cap structure formation in infectious bursal disease virus, a member of the birnavirus group Free

Abstract

The putative viral transcriptase p90 in infectious bursal disease virus (IBDV) was shown to form an enzyme- guanylate intermediate which is indicative of guanylyl- transferase activity. The p90-nucleotide bond is most likely to be a phosphodiester linkage, as it resisted treatment with HC1 and NHOH but was sensitive to NaOH. This is in contrast to phosphoamide bonds formed by reovirus cores. Methyltransferase activity was also demonstrated in IBDV, and is closely associated with transcription, suggesting that p90 may be a multifunctional enzyme.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-4-977
1990-04-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/4/JV0710040977.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-4-977&mimeType=html&fmt=ahah

References

  1. Abraham G., Rhodes D., Banerjee A. K. 1975; The 5'-terminal structure of the methylated mRNA synthesized in vitro by VSV. Cell 5:51–58
    [Google Scholar]
  2. Banerjee A. K. 1980; 5ʹ-terminal cap structure in eukaryotic messenger ribonucleic acids. Microbiological Reviews 44:175–205
    [Google Scholar]
  3. Becht H. 1980; Infectious bursal disease virus. Current Topics in Microbiology and Immunology 90:107–121
    [Google Scholar]
  4. Brown F. 1986; The classification and nomenclature of viruses: summary of results of meetings of the International Committee on Taxonomy of Viruses in Sendai, September 1984. Intervirology 25:141–143
    [Google Scholar]
  5. Cross R. K. 1983; Identification of a unique guanine-7-methyl- transferase in Semliki Forest virus infected cell extracts. Virology 130:452–463
    [Google Scholar]
  6. Dever T. E., Glynias M. J., Merrick W. C. 1987; GTP-binding domain: three consensus sequence elements with distinct spacing. Proceedings of the National Academy of Sciences U.S.A.: 841814–1818
    [Google Scholar]
  7. Dobos P., Hill B. J., Hallett R., Kells D. T. C., Becht H., Teninges D. 1979; Biophysical and biochemical characterization of five animal viruses with bisegmented double-stranded genomes. Journal of Virology 32:593–605
    [Google Scholar]
  8. Faust M., Hastings K. E. M., Millward S. 1975; m7GpppGmpCpUp at the 5ʹ terminus of reovirus mRNA. Nucleic Acids Research 2:1329–1343
    [Google Scholar]
  9. Furuichi Y., Shatkin A. J. 1976; Differential synthesis of blocked and unblocked 5ʹ-termini in reovirus mRNA: effect of pyrophosphate and pyrophosphatase. Proceedings of the National Academy of Sciences U.S.A.: 733448–3452
    [Google Scholar]
  10. Furuichi Y., Shatkin A. J. 1977; 5ʹ-Termini of reovirus mRNA: ability of viral cores to form caps post-transcriptionally. Virology 7:566–578
    [Google Scholar]
  11. Furuichi Y., Morgan M., Muthukrishnan S., Shatkin A. J. 1975; Reovirus messenger RNA contains a methylated, blocked 5ʹ- terminal structure: rn7G(5ʹ)ppp(5ʹ)GmpCp. Proceedings of the National Academy of Sciences U.S.A.: 72362–366
    [Google Scholar]
  12. Furuichi Y., Muthukrishnan S., Tomasz J., Shatkin A. J. 1976; Mechanism of formation of reovirus mRNA 5ʹ-terminal blocked and methylated sequence m7GpppGmpC. Journal of Biological Chemistry 251:5043–5053
    [Google Scholar]
  13. Furuichi Y., Lafiandara A., Shatkin A. J. 1977; 5ʹ-Terminal structure and mRNA stability. Nature; London: 266235–239
    [Google Scholar]
  14. Horikami S. M., Deferra F., Moyer S. A. 1984; Characterization of the infections of permissive cells by host range mutants of VSV defective in RNA methylation. Virology 138:1–15
    [Google Scholar]
  15. Kozak M. 1986; Regulation of protein synthesis in virus-infected animal cells. Advances in Virus Research 31:229–292
    [Google Scholar]
  16. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Mi S., Durbin R., Huang H. V., Rice C. M., Stollar V. 1989; Association of the sindbis virus RNA methyltransferase activity with the non-structural protein nsPl. Virology 170:385–391
    [Google Scholar]
  18. Moss B., Gershowitz A., Wei C.-M., Boone R. 1976; Formation of the guanylated and methylated 5ʹ-terminus of vaccinia virus mRNA. Virology 72:341–351
    [Google Scholar]
  19. Müller H., Becht H. 1982; Biosynthesis of virus-specific proteins in cells infected with infectious bursal disease virus and their significance as structural elements for infectious and incomplete particles. Journal of Virology 44:384–392
    [Google Scholar]
  20. Müller H., Nitschke R. 1987a; Molecular weight determination of the two segments of double-stranded RNA of infectious bursal disease virus, a member of the bimavirus group. Medical Microbiology and Immunology 176:113–121
    [Google Scholar]
  21. Müller H., Nitschke R. 1987b; The two segments of infectious bursal disease virus are circularized by a 90,000-Da protein. Virology 159:174–177
    [Google Scholar]
  22. Müller H., Scholtissek C., Becht H. 1979; The genome of infectious bursal disease virus consists of two segments of doublestranded RNA. Journal of Virology 31:584–589
    [Google Scholar]
  23. Müller H., Lange H., Becht H. 1986; Formation, characterization and interfering capacity of a small plaque mutant and of incomplete virus particles of infectious bursal disease virus. Virus Research 4:297–309
    [Google Scholar]
  24. Muzimoto K., Kaziro Y., Lipmann F. 1982; Reaction mechanism of mRNA guanylyltransferase from rat liver: isolation and characterization of a guanylyl-enzyme intermediate. Proceedings of the National Academy of Sciences U.S.A.: 791693–1697
    [Google Scholar]
  25. Nick H., Cursiefen D., Becht H. 1976; Structural and growth characteristics of infectious bursal disease virus. Journal of Virology 18:227–234
    [Google Scholar]
  26. Seliger L. S., Zheng K., Shatkin A. J. 1987; Complete nucleotide sequence of reovirus L2 gene and deduced amino acid sequence of viral mRNA guanylyltransferase. Journal of Biological Chemistry 262:16289–16293
    [Google Scholar]
  27. Shabarova Z. A. 1970; Synthetic nucleotide-peptides. Progress in Nucleic Acid Research and Molecular Biology 10:145–182
    [Google Scholar]
  28. Shatkin A. J. 1976; Capping of eucaryotic mRNAs. Cell 9:645–653
    [Google Scholar]
  29. Shatkin A. J. 1985; mRNA cap binding proteins: essential factors for initiating translation. Cell 40:223–224
    [Google Scholar]
  30. Shatkin A. J., Furuichi Y., Lafiandara A. J., Yamakawa M. 1983; Initiation of mRNA synthesis and 5ʹ-terminal modification of reovirus transcripts. In Double-stranded RNA Viruses pp 42–54 Compans R. W., Bishop D. H. L. Edited by New York: Elsevier Scientific;
    [Google Scholar]
  31. Shuman S. 1982; RNA capping by HeLa cell RNA guanylyltransferase - characterization of a covalent protein-guanylate intermediate. Journal of Biological Chemistry 257:7237–7245
    [Google Scholar]
  32. Shuman S., Hurwitz J. 1981; Mechanism of mRNA capping by vaccinia guanylyltransferase: characterization of an enzyme- guanylate intermediate. Proceedings of the National Academy of Sciences U.S.A.: 78187–191
    [Google Scholar]
  33. Spies U., Müller H., Becht H. 1987; Properties of RNA polymerase activity associated with infectious bursal disease virus and characterization of its reaction products. Virus Research 8:127–140
    [Google Scholar]
  34. Toyama R., Mizumoto K., Nakahara Y., Tasumo T., Kaziro Y. 1983; Mechanism of the mRNA guanylyltransferase reaction: isolation of NE-phospholysine and GMP (5ʹ-NE) lysine from the guanylylenzyme intermediate. EMBO Journal 2:2195–2201
    [Google Scholar]
  35. Venkatesan S., Moss B. 1982; Eukaryotic mRNA capping enzyme-guanyiate intermediate. Proceedings of the National Academy of Sciences U.S.A.: 79340–344
    [Google Scholar]
  36. Wang D., Furuichi Y., Shatkin A. J. 1982; Covalent guanylyl intermediate formed by HeLa cell mRNA capping enzyme. Molecular and Cellular Biology 2:993–1001
    [Google Scholar]
  37. Zarbl H., Millward S. 1983; The reovirus multiplication cycle. In The Reoviridae pp 107–196 Joklik W. K. Edited by New York: Plenum Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-4-977
Loading
/content/journal/jgv/10.1099/0022-1317-71-4-977
Loading

Data & Media loading...

Most cited Most Cited RSS feed