RT Journal Article SR Electronic(1) A1 Okamoto, Hiroaki A1 Omi, Shigeru A1 Wang, Yu A1 Imai, Mitsunobu A1 Mayumi, MakotoYR 1990 T1 Trans-complementation of the C gene of human and the P gene of woodchuck hepadnaviruses JF Journal of General Virology, VO 71 IS 4 SP 959 OP 963 DO https://doi.org/10.1099/0022-1317-71-4-959 PB Microbiology Society, SN 1465-2099, AB A 5 bp insertion was introduced into the BstEII site at nucleotide 2815 in DNA of hepatitis B virus (HBV) and a mutant HBV genome was produced, which coded for envelope and core proteins, but not for DNA polymerase, due to a frameshift. Cultured hepatoma cells (HepG2) were simultaneously transfected with a plasmid harbouring a tandem dimer of the mutant HBV DNA and another plasmid harbouring a tandem dimer of DNA of woodchuck hepatitis virus or duck hepatitis B virus. The replication of mutant HBV DNA, incapable of encoding DNA polymerase, was accomplished by cotransfecting woodchuck hepatitis virus DNA, but not by duck hepatitis B virus DNA. These results indicated a trans-complementation of the C and P genes in mammalian hepadnaviruses beyond a species barrier., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-71-4-959