@article{mbs:/content/journal/jgv/10.1099/0022-1317-71-4-959, author = "Okamoto, Hiroaki and Omi, Shigeru and Wang, Yu and Imai, Mitsunobu and Mayumi, Makoto", title = "Trans-complementation of the C gene of human and the P gene of woodchuck hepadnaviruses", journal= "Journal of General Virology", year = "1990", volume = "71", number = "4", pages = "959-963", doi = "https://doi.org/10.1099/0022-1317-71-4-959", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-71-4-959", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "A 5 bp insertion was introduced into the BstEII site at nucleotide 2815 in DNA of hepatitis B virus (HBV) and a mutant HBV genome was produced, which coded for envelope and core proteins, but not for DNA polymerase, due to a frameshift. Cultured hepatoma cells (HepG2) were simultaneously transfected with a plasmid harbouring a tandem dimer of the mutant HBV DNA and another plasmid harbouring a tandem dimer of DNA of woodchuck hepatitis virus or duck hepatitis B virus. The replication of mutant HBV DNA, incapable of encoding DNA polymerase, was accomplished by cotransfecting woodchuck hepatitis virus DNA, but not by duck hepatitis B virus DNA. These results indicated a trans-complementation of the C and P genes in mammalian hepadnaviruses beyond a species barrier.", }