Synthetic transcripts of tomato black ring virus satellite RNA (TBRV satRNA), isolate L, were prepared from cDNA cloned in the Bluescribe transcription vector. Transcripts with 49 (T49L) or two (T2GL) extra nucleotides at their 5′ ends and 42 extra nucleotides at their 3′ ends were able to induce, but to different extents, the synthesis of the satRNA-encoded 48K protein. However, when inoculated into together with TBRV L genomic RNAs, only T2GL was biologically active, in the presence or absence of a 5′ cap analogue in the transcription reactions. Analysis of the 5′ and 3′ termini of the satRNA isolated from plants showed that non-viral extensions were not maintained in the transcript progeny.


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