1887

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Volume 71, Issue 4

Control of expression of the varicella-zoster virus major immediate early gene Free

Abstract

The cis-acting DNA sequences and trans-acting proteins that control the expression of the major immediate early (IE) gene of varieella-zoster virus (VZV) were investigated. The location of the IE mRNA 5′ terminus was determined by primer extension and S1 nuclease analyses and the functional activities of DNA sequences upstream of this site were analysed by a transfection assay. The VZV IE promoter exhibited low activity in BHK and HeLa cells, but was transactivated by the herpes simplex virus type 1 (HSV-1) virion protein Vmw65. DNA sequences between positions −131 and +57 were responsible for promoter activity, whereas sequences between −410 and −131 mediated the response to Vmw65. Two short elements in the −410 to −131 region formed protein-DNA complexes with HeLa cell nuclear proteins and formed a ternary complex when Vmw65 was added. One of the elements, ATGTAAATGAAAT, possessed a strong similarity to the HSV-1 TAATGARAT. The VZV homologue of Vmw65, encoded by open reading frame (ORF) 10, failed to trans-activate expression from HSV-1 or VZV IE promoters and did not form a ternary complex with functional TAATGARAT elements and HeLa cell proteins. Therefore, stimulation of VZV IE transcription by Vmw65 can occur by a mechanism similar to that employed by HSV-1, but VZV ORF 10 does not function as a trans-activator of IE gene expression.

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© Society for General Microbiology 1990
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1990-04-01
2024-04-18
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References

  1. Ace C. I., Dalrymple M. A., Ramsay F. H., Preston V. G., Preston C. M. 1988; Mutational analysis of the herpes simplex virus type 1 trans-inducing factor Vmw65. Journal ofGeneral Virology 69:2595–2605
     [Google Scholar]
  2. Ace C. I., Mckee T. A., Ryan J. M., Cameron J. M., Preston C. M. 1989; Construction and characterization of a herpes simplex virus type 1 mutant unable to transinduce immediate-early gene expression. Journal of Virology 63:2260–2269
     [Google Scholar]
  3. Aprhys C. M., Cuifo D. M., O’Neill E. A., Kelly T. K., Hayward G. S. 1989; Overlapping octamer and TAATGARAT motifs in the VF65-response elements in herpes simplex virus immediate-early promoters represent independent binding sites for cellular nuclear factor III. Journal of Virology 63:2798–2812
     [Google Scholar]
  4. Baumruker T., Sturm R., Herr W. 1988; OBP100 binds remarkably degenerate octamer motifs through specific interactions with flanking sequences. Genes and Development 2:1400–1413
     [Google Scholar]
  5. Boshart M., Weber F., Jahn G., Dorsch-Hasler K., Fleckenstein B., Schaffner W. 1985; A very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus. Cell 41:521–530
     [Google Scholar]
  6. Campbell M. E. M., Preston C. M. 1987; DNA sequences which regulate the expression of the pseudorabies virus major immediate early gene. Virology 157:307–316
     [Google Scholar]
  7. Campbell M. E. M., Palfreyman J. W., Preston C. M. 1984; Identification of herpes simplex virus DNA sequences which encode a trans acting polypeptide responsible for stimulation of immediate early transcription. Journal of Molecular Biology 180:1–19
     [Google Scholar]
  8. Clements J. B., Watson R. J., Wilkie N. M. 1977; Temporal regulation of herpes simplex virus type 1 transcription: location of transcripts on the viral genome. Cell 12:275–285
     [Google Scholar]
  9. Cordingley M. G., Campbell M. E. M., Preston C. M. 1983; Functional analysis of a herpes simplex virus type 1 promoter: identification of far-upstream regulatory sequences. Nucleic Acids Research 11:2347–2365
     [Google Scholar]
  10. Corsalo C. M., Pearson M. L. 1981; Enhancing the efficiency of DNA-mediated gene transfer in mammalian cells. Somatic Cell Genetics 7:603–616
     [Google Scholar]
  11. Cousens D. J., Greaves R., Goding C. R., O’Hare P. 1989; The C-terminal 79 amino acids of the herpes simplex virus regulatory protein, Vmw65, efficiently activate transcription in yeast and mammalian cells in chimeric DNA-binding proteins. EM BO Journal 8:2337–2342
     [Google Scholar]
  12. Dalrymple M. A., Mcgeoch D. J., Davison A. J., Preston C. M. 1985; DNA sequence of the herpes simplex virus type 1 gene whose product is responsible for transcriptional activation of immediate early promoters. Nucleic Acids Research 13:7865–7879
     [Google Scholar]
  13. Davison A. J., Scott J. E. 1985; DNA sequence of the major inverted repeat in the varicella-zoster virus genome. Journal of General Virology 66:207–220
     [Google Scholar]
  14. Davison A. J., Scott J. E. 1986; The complete DNA sequence of varicella-zoster virus. Journal of General Virology 67:1759–1816
     [Google Scholar]
  15. Everett R. D. 1984; Trans activation of transcription by herpes virus products: requirement for two HSV-1 immediate early polypeptides for maximum activity. EMBO Journal 3:3135–3141
     [Google Scholar]
  16. Everett R. D., Dunlop M. 1984; Trans activation of plasmid- borne promoters by adenovirus and several herpes group viruses. Nucleic Acids Research 12:5969–5978
     [Google Scholar]
  17. Felser J. M., Kinchington P. R., Inchauspe G., Straus S. E., Ostrove J. M. 1988; Cell lines containing varicella-zoster virus open reading frame 62 and expressing the ‘IE’175 protein complement ICP4 mutants of herpes simplex virus type 1. Journal of Virology 62:2076–2082
     [Google Scholar]
  18. Fickenscher H., Stamminger T., Rüger R., Fleckenstein B. 1989; The role of a repetitive palindromic sequence element in the human cytomegalovirus major immediate early enhancer. Journal of General Virology 70:107–123
     [Google Scholar]
  19. Gaffney D. F., Mclaughlan J., Whitton J. L., Clements J. B. 1985; A modular system for the assay of transcription regulatory signals: the sequence TAATGARAT is required for herpes simplex virus immediate early gene activation. Nucleic Acids Research 13:7847–7863
     [Google Scholar]
  20. Gerster T., Roeder R. G. 1988; A herpes virus transactivating protein interacts with transcription factor OTF-1 and other cellular proteins. Proceedings of the National Academy of Sciences, U.S.A 85:6347–6351
     [Google Scholar]
  21. Gorman C. M., Moffatt L. F., Howard B. H. 1982; Recombinant genomes which express chloramphenicol acetyl transferase in mammalian cells. Molecular and Cellular Biology 2:1044–1051
     [Google Scholar]
  22. Greaves R., O’Hare P. 1989; Separation of requirements for protein-DNA complex assembly from those for functional activity in the herpes simplex virus regulatory protein Vmw65. Journal of Virology 63:1641–1650
     [Google Scholar]
  23. Honess R. W., Roizman B. 1974; Regulation of herpesvirus macromolecular synthesis. I. Cascade regulation of the synthesis of three groups of viral proteins. Journal of Virology 14:8–19
     [Google Scholar]
  24. Ihara S., Feldman L., Watanabe S., Ben-Porat T. 1983; Characterisation of the immediate-early functions of pseudorabies virus. Virology 131:437–454
     [Google Scholar]
  25. Kristie T. M., Roizman B. 1987; Host cell proteins bind to the cis- acting site required for virion mediated induction of herpes simplex virus 1 a genes. Proceedings of the National Academy of Sciences, U.S.A 84:71–75
     [Google Scholar]
  26. Lang J. C., Spandidos D. A., Wilkie N. M. 1984; Transcriptional regulation of a herpes simplex virus immediate early gene is mediated through an enhancer type sequence. EMBO Journal 3:389–395
     [Google Scholar]
  27. Mackem S., Roizman B. 1982; Structural features of the herpes simplex virus a gene 4, 0, and 27 promoter-regulatory sequences which confer a regulation on chimeric thymidine kinase genes. Journal of Virology 44:939–949
     [Google Scholar]
  28. Mcknight J. L. C., Kristie T. M., Roizman B. 1987; Binding of the virion protein mediating α gene induction in herpes virus 1-infected cells to its cis site requires cellular proteins. Proceedings of the National Academy of Sciences, U.S.A 84:7061–7065
     [Google Scholar]
  29. Marsden H. S., Campbell M. E. M., Haarr L., Frame M. C., Parris D. S., Murphy M., Hope R. G., Muller M. G., Preston C. M. 1987; The 65,000 Mr DNA-binding and virion trans-inducing proteins of herpes simplex virus type 1. Journal of Virology 61:2428–2437
     [Google Scholar]
  30. Muller M. T. 1987; Binding of the herpes simplex virus immediate early gene product ICP4 to its own transcription start site. Journal of Virology 61:858–865
     [Google Scholar]
  31. O’Hare P., Goding C. R. 1988; Herpes simplex virus regulatory elements and the immunoglobulin octamer domain bind a common factor and are both targets for virion transactivation. Cell 52:453–445
     [Google Scholar]
  32. O’Hare P., Hayward G. S. 1985a; Evidence for a direct role of both the 175Kand 110K immediate-early proteins of herpes simplex virus in the transactivation of the delayed-early promoters. Journal of Virology 53:752–760
     [Google Scholar]
  33. O’Hare P., Hayward G. S. 1985b; Three trans-acting regulatory proteins of herpes simplex virus modulate immediate-early gene expression in a pathway involving positive and negative feedback regulation. Journal of Virology 56:723–733
     [Google Scholar]
  34. O’Hare P., Hayward G. S. 1987; Comparison of upstream sequence requirements for positive and negative regulation of a herpes simplex virus immediate early gene by three virus-encoded trans-acting factors. Journal of Virology 61:190–199
     [Google Scholar]
  35. O’Hare P. C., Goding C. R., Haigh A. 1988; Direct combinatorial interaction between a herpes simplex virus regulatory protein and a cellular octamer-binding factor mediates specific induction of virus immediate-early gene expression. EMBO Journal 7:4231–4238
     [Google Scholar]
  36. Post L. E., Mackem S., Roizman B. E. 1981; Regulation of α genes of herpes simplex virus: expression of chimeric genes produced by fusion of thymidine kinase with a gene promoters. Cell 24:555–565
     [Google Scholar]
  37. Preston C. M. 1977; The cell free synthesis of herpesvirus-induced polypeptides. Virology 78:349–353
     [Google Scholar]
  38. Preston C. M. 1979; Control of herpes simplex virus type 1 mRNA synthesis in cells infected with wild-type virus or the temperature sensitive mutant tsK. Journal of Virology 29:275–284
     [Google Scholar]
  39. Preston C. M., Tannahill D. J. 1984; Effects of orientation and position on the activity of a herpes simplex virus immediate-early gene far upstream region. Virology 137:439–444
     [Google Scholar]
  40. Preston C. M., Cordingley M. G., Stow N. D. 1984; Analysis of DNA sequences which regulate the transcription of a herpes simplex virus immediate early gene. Journal of Virology 50:708–716
     [Google Scholar]
  41. Preston C. M., Frame M. C., Campbell M. E. M. 1988; A complex formed between cell components and an HSV structural polypeptide binds to a viral immediate early gene regulatory DNA sequence. Cell 52:425–434
     [Google Scholar]
  42. Pruijn G. J. M., van Driel W., van der Vliet P. C. 1986; Nuclear factor III, a novel sequence-specific DNA-binding protein from HeLa cells stimulating adenovirus DNA replication. Nature; London: 322656–659
     [Google Scholar]
  43. Rixon F. J., Clements J. B. 1982; Detailed structural analysis of two spliced HSV-1 immediate-early mRNAs. Nucleic Acids Research 10:2241–2256
     [Google Scholar]
  44. Sacks W. R., Greene C. C., Aschman D. P., Schaffer P. A. 1985; Herpes simplex virus type 1ICP27 is an essential regulatory protein. Journal of Virology 55:796–805
     [Google Scholar]
  45. Sadowski I., Ma J., Triezenberg S., Ptashne M. 1988; GAL4-VP16 is an unusually potent transcriptional activator. Nature; London: 335563–564
     [Google Scholar]
  46. Shiraki K., Hyman R. W. 1987; The immediate early proteins of varicella zoster virus. Virology 156:423–426
     [Google Scholar]
  47. Spaete R. R., Mocarski E. S. 1985; Regulation of cytomegalovirus gene expression: α and β promoters are trans activated by viral functions in permissive human fibroblasts. Journal of Virology 56:135–143
     [Google Scholar]
  48. Stow N. D., Murray M. D., Stow E. C. 1986; Cis-acting signals involved in the replication and packaging of herpes simplex virus type 1 DNA. In Cancer Cells 4 pp. 497–507 Botchan M., Grodzicker T., Sharp P. Edited by New York: Cold Spring Harbor Laboratory;
     [Google Scholar]
  49. Sturm R., Baumruker T., Franza B. R., Herr W. 1987; A lOOkDHeLa cell octamer binding protein (OBP100) interacts differently with two separate octamer-related sequences within the SV40 enhancer. Genes and Development 1:1147–1160
     [Google Scholar]
  50. Triezenberg S. J., Lamarco K. L., Mcknight S. L. 1988; Evidence of DNA: protein interactions that mediate HSV-1 immediate early gene activation by VP16. Genes and Development 2:730–742
     [Google Scholar]
  51. Watson R. J., Clements J. B. 1980; A herpes simplex virus type 1 function continuously required for early and late virus RNA synthesis. Nature; London: 285329–330
     [Google Scholar]
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Control of expression of the varicella-zoster virus major immediate early gene
J. Gen. Virol. 71, 897 (1990); https://doi.org/10.1099/0022-1317-71-4-897
/content/journal/jgv/10.1099/0022-1317-71-4-897
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