1887

Abstract

The SK-v cells, established from a premalignant vulvar lesion, contain human papillomavirus type 16 (HPV- 16) sequences integrated at a single cellular site and derive from a cell clone present Transcription of the HPV-16 genome in SK-v cells was analysed by cDNA heteroduplex mapping and sequencing, and by RNase mapping. Viral sequences were shown to be transcribed into virus-cell fusion messengers. The two major transcripts have a coding capacity for a truncated E6 protein, an E7 protein and an E1-E4 fusion protein, but differ in their 3′ virus-cell junction. Minor transcripts have a coding capacity for a full-length E6 protein and another truncated version of E6. The transcription pattern in the E6-E7 region was found to be the same both in SK-v cells and in CaSki cells, a line derived from an invasive cervical carcinoma. Immuno- precipitation experiments showed that the E6 protein (18K) and, predominantly, the E7 protein (20K) are expressed in SK-v cells as in CaSki cells. The E7 protein was found in a two- to threefold lower amount in SK-v cells, but showing the same half-life (about 1 h).

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1990-04-01
2024-03-05
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References

  1. Androphy E. J., Hubbert N. L., Schiller J. T., Lowy D. R. 1986; Identification of the HPV16 E6 protein from transformed mouse cells and human cervical carcinoma cell lines. EMBO Journal 6:989–992
    [Google Scholar]
  2. Baker C. C., Phelps W. C., Lindgren V., Braun M. J., Gonda M. A., Howley P. M. 1987; Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines. Journal of Virology 61:962–971
    [Google Scholar]
  3. Barbosa M. S., Wettstein F. O. 1988; Identification and characterization of the CRPV E7 protein expressed in COS-7 cells. Virology 165:134–140
    [Google Scholar]
  4. Beaudenon S., Kremsdorf D., Croissant O., Jablonska S., Wain-Hobson S., Orth G. 1986; A novel type of papillomavirus associated with genital neoplasia. Nature; London: 321246–249
    [Google Scholar]
  5. Beaudenon S., Kremsdorf D., Obalek S., Jablonska S., Pehau-Arnaudet G., Croissant O., Orth G. 1987; Plurality of genital human papillomaviruses: characterization of two new types with distinct biological properties. Virology 161:374–384
    [Google Scholar]
  6. Biggin M. D., Gibson T. J., Hong G. F. 1983; Buffer gradients gels and 35S label as an aid to rapid DNA sequence determination. Proceedings of the National Academy of Sciences, U.S.A 80:3963–3965
    [Google Scholar]
  7. Boshart M., Gissmann L., Ikenberg H., Kleinheinz A., Scheurlen W., zur Hausen H. 1984; A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO Journal 3:1151–1157
    [Google Scholar]
  8. Chen E. Y., Seeburg P. H. 1985; Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA 4:165–170
    [Google Scholar]
  9. Choo K.-B., Pan C.-C., Han S.-H. 1987; Integration of human papillomavirus type 16 into cellular DNA of cervical carcinoma: preferential deletion of the E2 gene and invariable retention of the long control region and of the E6/E7 open reading frames. Virology 161:259–261
    [Google Scholar]
  10. Crook T., Storey A., Almond N., Osborn K., Crawford L. 1988; Human papillomavirus type 16 cooperates with activated ras and fos oncogenes in the hormone-dependent transformation of primary mouse cells. Proceedings of the National Academy of Sciences, U.S.A 85:8820–8824
    [Google Scholar]
  11. Crum C. P., Nuovo G., Friedman D., Silverstein S. J. 1988; Accumulation of RNA homologous to human papillomavirus type 16 open reading frames in genital precancers. Journal of Virology 62:84–90
    [Google Scholar]
  12. Dale R. M. K., Mcclure B. A., Houchins J. P. 1985; A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the com mitochondrial 18S rDNA. Plasmid 13:31–40
    [Google Scholar]
  13. Davis R. W., Simon M., Davidson N. 1971; Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. Methods in Enzymology 21D:413–418
    [Google Scholar]
  14. Dürst M., Gissmann L., Ikenberg H., Zur Hausen H. 1983; A papillomavirus DNA from a cervical carcinoma and its prevalence in cancer biopsy samples from different geographic regions. Proceedings of the National Academy of Sciences, U.S.A 80:3812–3815
    [Google Scholar]
  15. Dürst M., Schwarz E., Gissmann L. 1986; Integration and persistence of human papillomavirus DNA in genital tumors. In Viral Etiology of Cervical Cancer pp. 273–280 Peto R., zur Hausen H. Edited by New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  16. Durst M., Dzarlieva-Petrusevska R. T., Boukamp P., Fusenig N. E., Gissmann L. 1987; Molecular and cytogenetic analysis of immortalized human primary keratinocytes obtained after transfection with human papillomavirus type 16 DNA. Oncogene 1:251–256
    [Google Scholar]
  17. Dyson N., Howley P. M., Munger K., Harlow E. 1989; The human papilloma virus-16 oncoprotein is able to bind to the retinoblastoma gene product. Science 243:934–937
    [Google Scholar]
  18. El Awady M. K., Kaplan J. B., O’Brien J., Burk R. D. 1987; Molecular analysis of integrated human papillomavirus 16 sequences in the cervical cancer cell line SiHa. Virology 159:389–398
    [Google Scholar]
  19. Gubler U., Hoffman B. J. 1983; A simple and very effective method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  20. Huynh T. V., Young R. A., Davis R. W. 1984; Constructing and screening cDNA libraries in lambda gt10 and lambda gtl 1. In DNA Cloning Techniques: A Practical Approach 1 pp. 49–78 Glover D. Edited by Oxford: IRL Press;
    [Google Scholar]
  21. Inagaki Y., Tsunokawa Y., Takebe N., Nawa H., Nakanishi S., Terada M., Sugimura T. 1988; Nucleotide sequences of cDNAs for human papillomavirus type 18 transcripts in HeLa cells. Journal of Virology 62:1640–1646
    [Google Scholar]
  22. Jurka J., Smith T. 1988; A fundamental division in the Alu family of repeated sequences. Proceedings of the National Academy of Sciences. U.S.A 85:4775–4778
    [Google Scholar]
  23. Le J.-Y., Defendi V. 1988; A viral-cellular junction fragment from a human papillomavirus type 16-positive tumor is competent in transformation of NIH 3T3 cells. Journal of Virology 62:4420–4426
    [Google Scholar]
  24. Lehn H., Krieg P., Sauer G. 1985; Papillomavirus genomes in human cervical tumors: analysis of their transcriptional activity. Proceedings of the National Academy of Sciences, U.S.A 82:5540–5544
    [Google Scholar]
  25. Lipman D. J., Pearson W. R. 1985; Rapid and sensitive protein similarity searches. Science 227:1435–1440
    [Google Scholar]
  26. Lorincz A. T., Lancaster W. D., Temple G. F. 1986; Cloning and characterization of the DNA of a new human papillomavirus from a woman with dysplasia of the uterine cervix. Journal of Virology 58:225–229
    [Google Scholar]
  27. Lorincz A. T., Quinn A. P., Lancaster W. D., Temple G. F. 1987; A new type of papillomavirus associated with cancer of the uterine cervix. Virology 159:187–190
    [Google Scholar]
  28. Mccance D. J., Kopan R., Fuchs E., Laimins L. A. 1988; Human papillomavirus type 16 alters human epithelial cell differentiation in vitro. Proceedings of the National Academy of Sciences, U.S.A 85:7169–7173
    [Google Scholar]
  29. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  30. Matsukura T., Kanda T., Furuno A., Yoshikawa H., Kawana T., Yoshiike K. 1986; Cloning of monomeric human papillomavirus type 16 DNA integrated within cell DNA from a cervical carcinoma. Journal of Virology 58:979–982
    [Google Scholar]
  31. Melton D. A., Krieg P. A., Rebagliati M. R., Maniatis T., Zinn K., Green M. R. 1984; Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Research 12:7035–7056
    [Google Scholar]
  32. Pattillo R. A., Hussa R. O., Story M. T., Ruckert A. C. F., Shalaby M. R., Mattingly R. F. 1977; Tumor antigen and human chorionic gonadotropin in CaSki cells: a new epidermoid cervical cancer cell line. Science 196:1456–1458
    [Google Scholar]
  33. Phelps W. C., Yee C. L., Münger K., Howley P. M. 1988; The human papillomavirus type 16 E7 gene encodes transactivation and transformation functions similar to those of adenovirus E1 A. Cell 53:539–547
    [Google Scholar]
  34. Sanger F., Coulson A. R., Barrell B. G., Smith A. J. H., Roe B. A. 1980; Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. Journal of Molecular Biology 143:161–178
    [Google Scholar]
  35. Sastre-Garau X., Schneider-Maunoury S., Couturier J., Orth G. 1990; Human papillomavirus type 16 DNA is integrated into chromosome region 12q 14-q 15 in a cell line derived from a vulvar intraepithelial neoplasia. Cancer Genetics and Cytogenetics in press
    [Google Scholar]
  36. Schmid C. W., Jelinek W. R. 1982; The Alu family of dispersed repetitive sequences. Science 216:1065–1070
    [Google Scholar]
  37. Schneider-Gädicke A., Schwarz E. 1986; Different human cervical carcinoma cell lines show similar transcription patterns of human papillomavirus type 18 early genes. EMBO Journal 5:2285–2292
    [Google Scholar]
  38. Schneider-Maunoury S., Croissant O., Orth G. 1987; Integration of human papillomavirus type 16 DNA sequences: a possible early event in the progression of genital tumors. Journal of Virology 61:3295–3298
    [Google Scholar]
  39. Schwarz E., Freese U. K., Gissmann L., Mayer W., Roggenbuck B., Stremlau A., zur Hausen H. 1985; Structure and transcription of human papillomavirus sequences in cervical carcinoma cells. Nature; London: 314111–114
    [Google Scholar]
  40. Seedorf K., Krämmer G., Dürst M., Suhai S., Röwekamp W. G. 1985; Human papillomavirus type 16 DNA sequence. Virology 145:181–185
    [Google Scholar]
  41. Seedorf K., Oltersdorf T., Krämmer G., Röwekamp W. 1986; Identification of early proteins of the human papilloma viruses type 16 (HPV-16) and type 18 (HPV-18) in cervical carcinoma cells. EMBO Journal 6:139–144
    [Google Scholar]
  42. Shirasawa H., Tomita Y., Kubota K., Kazai T., Sekiya S., Takamizawa H., Simizu B. 1988; Transcriptional differences of the human papillomavirus type 16 genome between precancerous lesions and invasive carcinomas. Journal of Virology 62:1022–1027
    [Google Scholar]
  43. Smotkin D., Wettstein F. 1986; Transcription of human papillomavirus type 16 early genes in a cervical cancer and a cancer-derived cell line and identification of the E7 protein. Proceedings of the National Academy of Sciences, U.S.A 83:4680–4684
    [Google Scholar]
  44. Smotkin D., Wettstein F. O. 1987; The major human papillomavirus protein in cervical cancers is a cytoplasmic phospho-protein. Journal of Virology 61:1686–1689
    [Google Scholar]
  45. Smotkin D., Prokoph H., Wettstein F. O. 1989; Oncogenic and nononcogenic human genital papillomaviruses generate the E7 mRNA by different mechanisms. Journal of Virology 63:1441–1447
    [Google Scholar]
  46. Thierry F., Yaniv M. 1987; The BPV1-E2 trans-acting protein can be either an activator or a repressor of the HPV18 regulatory region. EMBO Journal 6:3391–3397
    [Google Scholar]
  47. Woodworth C. D., Bowden P. E., Doniger J., Pirisi L., Barnes W., Lancaster W. D., Dipaolo J. A. 1988; Characterization of normal human exocervical epithelial cells immortalized in vitro by papillomavirus types 16 and 18 DNA. Cancer Research 48:4620–4628
    [Google Scholar]
  48. Yajima H., Noda T., de Villiers E.-M., Yajima A., Yamamoto K., Noda K., Ito Y. 1988; Isolation of a new type of human papillomavirus (HPV52b) with a transforming activity from cervical cancer tissue. Cancer Research 48:7164–7172
    [Google Scholar]
  49. zur Hausen H., Schneider A. 1987; The role of papillomaviruses in human anogenital cancer. In The Papovaviridae 2 The papillomaviruses pp. 245–263 Salzman M. P., Howley P. M. Edited by New York: Plenum Press;
    [Google Scholar]
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