1887

Journal of General Virology

Volume 71, Issue 4

Host cell proteins required for measles virus reproduction Free

Abstract

We have developed a cell-free system derived from measles virus-infected cells that supported the transcription and replication of measles virus RNA The data suggest that tubulin may be required for these reactions, since an anti--tubulin monoclonal antibody inhibited viral RNA synthesis and the addition of purified tubulin stimulated measles virus RNA synthesis Tubulin may be a subunit of the viral RNA polymerase, since two different anti-tubulin antibodies, one specific for the - and another specific for the -subunit of tubulin, coimmunoprecipitated the measles virus L protein as well as tubulin from extracts of measles virus-infected cells. Other experiments further implicated actin in the budding process during virus maturation, as there appeared to be a specific association of actin only with nucleocapsids that have terminated RNA synthesis, which is presumably a prerequisite to budding.

  • Received:
  • Accepted:
  • Published Online:
© Society for General Microbiology 1990
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-4-775
1990-04-01
2021-10-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/4/JV0710040775.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-4-775&mimeType=html&fmt=ahah

References

  1. Alkhatib G., Briedis D. J. 1986; The predicted primary structure of the measles virus hemagglutinin. Virology 150:479–490
     [Google Scholar]
  2. Baker S. C., Moyer S. A. 1988; Encapsidation of Sendai virus genome RNAs by purified NP protein during in vitroreplication. Journal of Virology 62:834–838
     [Google Scholar]
  3. Banerjee A. K. 1987; Transcription and replication of rhabdo-viruses. Microbiological Reviews 51:66–87
     [Google Scholar]
  4. Bellini W. J., Englund G., Rozenblatt S., Arnheiter H., Richardson C. D. 1985; Measles virus P gene codes for two proteins. Journal of Virology 53:908–919
     [Google Scholar]
  5. Bellini W. J., Englund G., Richardson C. D., Rozenblatt S., Lazzarini R. A. 1986; Matrix genes of measles virus and canine distemper virus; cloning, nucleotide sequences and deduced amino acid sequences. Journal of Virology 58:408–416
     [Google Scholar]
  6. Billeter M. A., Backzo K., Schmid A., Ter Meulen V. 1984; Cloning of DNA corresponding to four different measles virus genomic regions. Virology 132:147–159
     [Google Scholar]
  7. Bohn W., Rutter G., Hohenberg H., Mannweiler K., Nobis P. 1986; Involvement of actin filaments in budding of measles virus: studies on cytoskeletons of infected cells. Virology 149:91–106
     [Google Scholar]
  8. Carlsen S. R., Peluso R. W., Moyer S. A. 1985; In vitroreplication of Sendai virus wild-type and defective interfering particle genome RNAs. Journal of Virology 54:493–500
     [Google Scholar]
  9. Cattaneo R., Kaelin K., Baczko K., Billeter M. A. 1989; Measles virus editing provides an additional cysteine-rich protein. Cell 56:759–764
     [Google Scholar]
  10. Chatterjee P. K., Cervera M. M., Penman S. 1984; Formation of vesicular stomatitis virus nucleocapsid from cytoskeletal framework-bound N protein; possible model for structural assembly. Molecular and Cellular Biology 4:2231–2234
     [Google Scholar]
  11. Dethlefsen L., Kolakofsky D. 1983; In vitro synthesis of the nonstructural C protein of Sendai virus. Journal of Virology 46:321–324
     [Google Scholar]
  12. Dowling P. C., Blumberg B. M., Menonna J., Adamus J. E., Cook P. J., Crowley J. C., Kolakofsky D., Cook S. D. 1986; T ranscriptional map of the measles virus genome. Journal of General Virology 67:1987–1992
     [Google Scholar]
  13. Gentry N., Busserau F. 1980; Is cytoskeleton involved in vesicular stomatitis virus reproduction?. Journal of Virology 34:777–781
     [Google Scholar]
  14. Gerald C., Buckland R., Barker R., Freeman G., Wild T. F. 1986; Measles virus haemagglutinin gene: cloning, complete nucleotide sequence analysis and expression in COS cells. Journal of General Virology 67:2695–2703
     [Google Scholar]
  15. Giuffre R. M., Tovell D. R., Kay C. M., Tyrrell D. L. J. 1982; Evidence for an interaction between the membrane protein of a paramyxovirus and actin. Journal of Virology 42:963–968
     [Google Scholar]
  16. Glazier K., Raghow R., Kingsbury D. W. 1977; Regulation of Sendai virus transcription: evidence for a single promoter in vivo. Journal of Virology 21:863–871
     [Google Scholar]
  17. Gundesen G. G., Kalnoski M. H., Bulinski J. C. 1984; Distinct populations of microtubules: tyrosinated and nontyrosinated alpha tubulin are distributed dilferently in vivo. Cell 38:779–789
     [Google Scholar]
  18. Hamaguchi M., Nishikawa K., Toyoda T., Yoshida T., Hanaichi T. 1985; Transcriptive complex of Newcastle disease virus. II. Structural and functional assembly associated with the cytoskeletal framework. Virology 147:295–308
     [Google Scholar]
  19. Hill V. M., Harmon S. A., Summers D. F. 1986; Stimulation of vesicular stomatitis virus in vitroRNA synthesis by microtubule-associated proteins. Proceedings of the National Academy of Sciences U.S.A.: 835410–5413
     [Google Scholar]
  20. Leppert M., Rittenhouse L., Perrault J., Summers D. F., Kolakofsky D. 1979; Plus- and minus-strand leader RNAs in negative strand virus infected cells. Cell 18:735–747
     [Google Scholar]
  21. Marx P. A., Portner A., Kingsbury D. W. 1974; Sendai virus transcriptase complex: polypeptide composition and inhibition by virion envelope proteins. Journal of Virology 13:107–112
     [Google Scholar]
  22. Mountcastle W. E., Choppin P. W. 1977; A comparison of four measles virus strains. Virology 78:463–474
     [Google Scholar]
  23. Moyer S. A., Baker S. C., Lessard J. L. 1986; Tubulin: a factor necessary for the synthesis of both Sendai virus and vesicular stomatitis virus RNAs. Proceedings of the National Academy of Sciences U.S.A.: 835405–5409
     [Google Scholar]
  24. Peluso R. W., Moyer S. A. 1983; Initiation and replication of vesicular stomatitis virus genome RNA in a cell free system. Proceedings of the National Academy of Sciences U.S.A.: 803192–3202
     [Google Scholar]
  25. Peluso R. W., Moyer S. A. 1988; Viral proteins required for the in vitroreplication of vesicular stomatitis virus defective interfering particle genome RNA. Virology 162:369–376
     [Google Scholar]
  26. Ray J., Fujinami R. S. 1987; Characterization of in vitrotranscription and transcriptional products of measles virus. Journal of Virology 61:3381–3387
     [Google Scholar]
  27. Richardson C. D., Berkovich A., Rozenblatt S., Bellini W. J. 1985; Use of antibodies directed against synthetic peptides for identifying cDNA clones, establishing reading frames and deducing the gene order of measles virus. Journal of Virology 54:186–193
     [Google Scholar]
  28. Rima B. K., Baczko K., Clarke D. K., Curran M. D., Martin S. J., Billeter M. A., Ter Meulen V. 1986; Characterization of clones for the sixth (L) gene and a transcription map for morbilliviruses. Journal of General Virology 67:1971–1978
     [Google Scholar]
  29. Rozenblatt S., Eizenberg O., Ben-Levy R., Lavie V., Bellini W. J. 1985; Sequence homology within the morbilliviruses. Journal of Virology 53:684–690
     [Google Scholar]
  30. Seifried A. S., Albrecht P., Milstien J. B. 1978; Characterization of an RNA-dependent RNA polymerase activity associated with measles virus. Journal of Virology 25:781–787
     [Google Scholar]
  31. Shioda T., Hidaka Y., Kanda T., Shibuta H., Nomota A., Iwasaki K. 1983; Sequence of 3687 nucleotides from the 3ʹ end of Sendai virus genome and the predicted amino acid sequence of viral NP, P and C proteins. Nucleic Acids Research 11:7317–7330
     [Google Scholar]
  32. Shioda T., Iwasaki K., Shibuta H. 1986; Determination of the complete nucleotide sequence of the Sendai virus genome RNA and the predicted amino acid sequences of the F, HN and L proteins. Nucleic Acids Research 14:1545–1563
     [Google Scholar]
  33. Simonsen C. C., Batt-Humphries S., Summers D. F. 1979; RNA synthesis of vesicular stomatitis virus-infected cells: in vivoregulation of replication. Journal of Virology 31:124–130
     [Google Scholar]
  34. Stallcup K. C., Wechsler S. L., Fields B. N. 1979; Purification of measles virus and characterization of subviral components. Journal of Virology 30:166–176
     [Google Scholar]
  35. Stallcup K. C., Raine C. S., Fields B. N. 1983; Cytochalasin B inhibits the maturation of measles virus. Virology 124:59–74
     [Google Scholar]
  36. Stone H. O., Portner A., Kingsbury D. W. 1971; Ribonucleic acid transcriptase in Sendai virions and infected cells. Journal of Virology 8:174–180
     [Google Scholar]
  37. Tyrrell D. L. J., Norrby E. 1978; Structural polypeptides of measles virus. Journal of General Virology 39:219–229
     [Google Scholar]
  38. Udem S., Cook K. A. 1984; Isolation and characterization of measles virus intracellular nucleocapsid RNA. Journal of Virology 49:57–65
     [Google Scholar]
  39. Wang E., Wolf B. A., Lamb R. A., Choppin P. W., Goldberg R. A. 1976; The presence of actin in enveloped viruses. In Cell Motility, Cold Spring Harbor Conferences on Cell Proliferation 3 pp 589–599 Goldman R., Pollard T., Rosenbaum J. Edited by New York: Cold Spring Harbor Laboratory;
     [Google Scholar]
  40. Wechsler S. L., Fields B. N. 1978; Intracellular synthesis of measles virus specified polypeptides. Journal of Virology 25:285–297
     [Google Scholar]
  41. Wong T. C., Hirano A. 1987; Structure and function of the bicistronic RNA encoding the phosphoprotein and matrix protein of measles virus. Journal of Virology 61:584–589
     [Google Scholar]
  42. Yoshikawa Y., Mizumoto K., Yamanouchi K. 1986; Characterization of messenger RNAs of measles virus. Journal of General Virology 67:2807–2812
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-4-775
Loading
Host cell proteins required for measles virus reproduction
J. Gen. Virol. 71, 775 (1990); https://doi.org/10.1099/0022-1317-71-4-775
/content/journal/jgv/10.1099/0022-1317-71-4-775
/content/journal/jgv/10.1099/0022-1317-71-4-775
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error