1887

Abstract

The genomes of an Australian and a Canadian isolate of potato leafroll virus have been cloned and sequenced. The sequences of both isolates are similar (about 93%), but the Canadian isolate (PLRV-C) is more closely related (about 98% identity) to a Scottish (PLRV-S) and a Dutch isolate (PLRV-N) than to the Australian isolate (PLRV-A). The 5′-terminal 18 nucleotide residues of PLRV-C, PLRV-A, PLRV-N and beet western yellows virus have 17 residues in common. In contrast, PLRV-S shows no obvious similarity in this region. PLRV-A and PLRV-C genomic sequences have localized regions of marked diversity, in particular a 600 nucleotide residue sequence in the polymerase gene. These data provide a world-wide perspective on the molecular biology of PLRV strains and their comparison with other luteoviruses and related RNA plant viruses suggests that there are two major subgroups in the plant luteoviruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-3-719
1990-03-01
2022-10-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/3/JV0710030719.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-3-719&mimeType=html&fmt=ahah

References

  1. Brierly I., Digard P., Inglis S. C. 1989; Characterization of an efficient coronavirus ribosomal frameshifting signal: requirement for an RNA pseudoknot. Cell 57:537–547
    [Google Scholar]
  2. Craigen W. J., Caskey C. T. 1987; Translational frameshifting: where will it stop?. Cell 50:1–2
    [Google Scholar]
  3. Devereaux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  4. Froman M. A., Dush M. K., Martin G. R. 1988; Rapid production of full length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proceedings of the National Academy of Sciences, U.S.A 85:8998–9002
    [Google Scholar]
  5. Gubler U., Hoffman B. J. 1983; A simple and very effective method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  6. Jacks T., Madhani H. D., Masiarz F. R., Varmus H. E. 1988; Signals for ribosomal frameshifting in the Rous sarcoma virus gag-pol region. Cell 55:447–458
    [Google Scholar]
  7. Kawchuk L. M., Martin R. R., Rochon D. M., Mcpherson J. 1989; Identification and characterization of the potato leafroll virus putative coat protein gene. Journal of General Virology 70:783–788
    [Google Scholar]
  8. Matthews R. E. F. 1982; Classification and nomenclature of viruses. Intervirology 17:140–141
    [Google Scholar]
  9. Mayo M. A., Barker H., Robinson D. J., Tamada T., Harrison B. D. 1982; Evidence that potato leafroll virus is positive-stranded, is linked to a small protein and does not contain polyadenylate. Journal of General Virology 59:163–167
    [Google Scholar]
  10. Mayo M. A., Robinson D. J., Jolly C. A., Hyman L. 1989; Nucleotide sequence of potato leafrollluteovirus RNA. Journal of General Virology 70:1037–1051
    [Google Scholar]
  11. Miller W. A., Waterhouse P. M., Gerlach W. L. 1988a; Sequence and organization of barley yellow dwarf virus genomic RNA. Nucleic Acids Research 16:6097–6111
    [Google Scholar]
  12. Miller W. A., Waterhouse P. M., Kortt A. A., Gerlach W. L. 1988b; Sequence and identification of the barley yellow dwarf virus coat protein gene. Virology 169:306–309
    [Google Scholar]
  13. Oxender D. L., Zurawski G., Yanofsky C. 1979; Attenuation in the E. coli tryptophan operon: role of RNA structure involving the tryptophan codon region. Proceedings of the National Academy of Sciences, U.S.A 76:5524–5528
    [Google Scholar]
  14. Rochow W. F., Duffus J. E. 1981; Luteoviruses and yellows disease. In Handbook of Plant Virus Infections and Comparative Diseases pp. 147–170 Kurstak E. Edited by Amsterdam: Elsevier Biomedical;
    [Google Scholar]
  15. Rowhani A., Stace-Smith R. 1979; Purification and characterization of potato leafroll virus. Virology 98:45–54
    [Google Scholar]
  16. Sanger F., Coulson A. R., Barrell B. G., Smith A. J. H., Roe B. A. 1980; Cloning in a single-stranded bacteriophage as an aid to rapid DNA sequencing. Journal of Molecular Biology 143:161–178
    [Google Scholar]
  17. Staden R. 1982; Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucleic Acids Research 10:4731–4751
    [Google Scholar]
  18. Thomas J. E. 1984; Characterization of an Australian isolate of tomato yellow top virus. Annals of Applied Biology 104:79–86
    [Google Scholar]
  19. Van Der Wilk F., Huisman M. J., Cornelissen B. J. C., Huttinga H., Goldbach R. 1989; Nucleotide sequence and organization of potato leafroll virus genomic RNA. FEBS Letters 245:51–56
    [Google Scholar]
  20. Veidt I., Lot H., Leiser M., Scheidecker D., Guilley H., Richards K., Jonard G. 1988; Nucleotide sequence of beet western yellows virus RNA. Nucleic Acids Research 16:9917–9932
    [Google Scholar]
  21. Waterhouse P. M., Gerlach W. L., Miller W. A. 1986; Serotype-specific and general luteovirus probes from cloned cDNA sequences of barley yellow dwarf virus. Journal of General Virology 67:1273–1281
    [Google Scholar]
  22. Waterhouse P. M., Gildow F. E., Johnstone G. R. 1988; The luteovirus group. CMI/AAB Descriptions of Plant Viruses339
    [Google Scholar]
  23. Wu S., Rinehart C. A., Kaesberg P. 1987; Sequence and organization of southern bean mosaic virus genomic RNA. Virology 161:73–80
    [Google Scholar]
  24. Xiong Z., Lommel S. A. 1989; The complete nucleotide sequence and genome organization of red clover necrotic mosaic virus RNA-1. Virology 171:543–554
    [Google Scholar]
  25. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103–109
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-3-719
Loading
/content/journal/jgv/10.1099/0022-1317-71-3-719
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error