1887

Abstract

The porcine paramyxovirus is a newly identified agent of a fatal disease in piglets, endemic in Mexico since 1980, where it was seen around the town of La Piedad, Michoacan, Mexico (hence LPM virus). At least six [S]methionine-labelled proteins could be resolved by SDS-PAGE and five of them were clearly immuno-precipitated. Selective labelling of LPMV-infected cells with [H]glucosamine revealed two bands with an of about 66K and 59K, corresponding to the two viral glycoproteins, the haemagglutinin—neuraminidase protein and the fusion protein. Labelling of virus with [P]orthophosphate disclosed one band with an of 52K, corresponding to the phosphoprotein. Analysis of nucleocapsids obtained from purified virus or from a permanently infected cell line revealed one major band with an of 68K, the nucleoprotein. Two other proteins were also identified, the large protein and the matrix protein, with apparent of about 200K and 40K, respectively. The protein migration pattern of LPMV was compared, by SDS-PAGE, with that of Newcastle disease virus, bovine parainfluenza 3 virus and Sendai virus. Differences in the of LPMV proteins and the proteins of these paramyxoviruses were observed. We propose that LPMV should be classified as a novel member of the genus .

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-3-609
1990-03-01
2019-11-15
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-3-609
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error