1887

Abstract

The porcine paramyxovirus is a newly identified agent of a fatal disease in piglets, endemic in Mexico since 1980, where it was seen around the town of La Piedad, Michoacan, Mexico (hence LPM virus). At least six [ S] methionine-labelled proteins could be resolved by SDS-PAGE and five of them were clearly immuno- precipitated. Selective labelling of LPMV-infected cells with [H]glucosamine revealed two bands with an of about 66K and 59K, corresponding to the two viral glycoproteins, the haemagglutinin-neuramini- dase protein and the fusion protein. Labelling of virus with [P]orthophosphate disclosed one band with an of 52K, corresponding to the phosphoprotein.

Analysis of nucleocapsids obtained from purified virus or from a permanently infected cell line revealed one major band with an of 68K, the nucleoprotein. Two other proteins were also identified, the large protein and the matrix protein, with apparent of about 200K and 40K, respectively. The protein migration pattern of LPMV was compared, by SDS-PAGE, with that of Newcastle disease virus, bovine parainfluenza 3 virus and Sendai virus. Differences in the of LPMV proteins and the proteins of these paramyxoviruses were observed. We propose that LPMV should be classified as a novel member of the genus

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1990-03-01
2022-01-20
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References

  1. Appel M. 1987; Canine distemper virus. In Virus Infections of Carnivores 1 pp 133–159 Appel M. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  2. Baker S. C., Moyer S. A. 1988; Encapsidation of Sendai virus genome RNAs by purified NP protein during in vitroreplication. Journal of Virology 62:834–838
    [Google Scholar]
  3. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of proteins utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–258
    [Google Scholar]
  4. Brandly C. A. 1964; Recognition of Newcastle disease as a new disease. In Newcastle Disease Virus: An Evolving Pathogen pp 53–64 Hanson R. P. Edited by Madison: University of Wisconsin Press;
    [Google Scholar]
  5. Elango N. 1989; The mumps virus nucleocapsid mRNA sequence and homology among the Paramyxoviridae. Virus Research 12:7786
    [Google Scholar]
  6. Hamaguchi M., Yoshida T., Nishikawa K., Naruse H., Nagai Y. 1983; Transcriptive complex of Newcastle disease virus. Virology 128:105–117
    [Google Scholar]
  7. Horne R. E., Waterson A. P. 1960; A helical structure in mumps, Newcastle disease and Sendai viruses. Journal of Molecular Biology 2:75–77
    [Google Scholar]
  8. Kilham L. 1949; Mumps meningoencephalitis with and without parotitis. American Journal of Diseases of Children 78:324–333
    [Google Scholar]
  9. Kolakofsky D. 1976; Isolation and characterization of Sendai virus DI-RNAs. Cell 8:547–555
    [Google Scholar]
  10. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  11. Matthews R. E. F. 1982; Classification and nomenclature of viruses. Intervirology 17:1–181
    [Google Scholar]
  12. Moreno-Lþpez J., Correa-Giron P., Martinez A., Eriksson A. 1986; Characterization of a paramyxovirus isolated from the brain of a piglet in Mexico. Archives of Virology 91:221–231
    [Google Scholar]
  13. Nagai N., Klenk H.-D., Rott R. 1976; Proteolytic cleavage of the viral glycoproteins and its significance for the virulence of Newcastle disease virus. Virology 72:494–508
    [Google Scholar]
  14. Oglesbee M., Tatalick L., Ringlers S., Rice J., Krakowka S. 1989; Rapid isolation of morbilli nucleocapsid for genomic RNA cDNA cloning and the production of specific core protein antisera. Journal of Virological Methods 24:285–300
    [Google Scholar]
  15. Panigrahi P., Mohanty S. B., Maheshwari R. K., Friedman R. M. 1987; Structural proteins of bovine parainfluenza-3 virus. Veterinary Microbiology 13:205–210
    [Google Scholar]
  16. Rydbeck R., Örvell C., Löve A., Norrby E. 1986; Characterization of four parainfluenza virus type 3 proteins by use of monoclonal antibodies. Journal of General Virology 67:1531–1542
    [Google Scholar]
  17. Scheid A., Choppin P. W. 1977; Two disulfide linked polypeptide chains constitute the active F protein of paramyxovirus. Virology 80:54–66
    [Google Scholar]
  18. Shaffer M. F., Rake G., Hodes H. L. 1942; Isolation of virus from a patient with fatal encephalitis complicating measles. American Journal of Diseases of Children 64:815–819
    [Google Scholar]
  19. Smith G. W., Hightower L. E. 1981; Identification of the P proteins and other disulfide-linked and phosphorylated proteins of Newcastle disease virus. Journal of Virology 37:256–267
    [Google Scholar]
  20. Stephano H. A., Gay G. M., Ramirez C., Maqueda J. 1981; An outbreak of encephalitis in piglets produced by an hemagglutinating virus. Proceedings of the International Pig Veterinary Society Congress, Mexico p 153
    [Google Scholar]
  21. Stephano H. A., Gay G. M., Ramirez T. C. 1988; Encephalomyelitis, reproductive failure and corneal opacity (blue eyes) in pigs, associated with a paramyxovirus infection. Veterinary Record 122:6–10
    [Google Scholar]
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