1887

Abstract

Synthesis of the adenovirus type 12 El A and E1B tumour antigens was compared in productively infected human (KB) cells and in abortively infected baby hamster kidney (BHK) cells. By the use of anti-peptide antibodies, the El A tumour antigens were easily detectable in infected KB extracts as early as 6 h postinfection, but were not detectable in infected BHK extracts until 12 h post-infection. The level of El A tumour antigens detected in BHK extracts was 10 to 15 % of that detected in KB extracts. The level of the E1B 163R (19K) tumour antigen was also lower in BHK extracts: 5 to 10 % of that detected in KB extracts. Stability of the El A tumour antigens was not significantly different in the two infected cell species, indicating that the lower El A level during abortive infection was due to a lower rate of synthesis of these proteins. These data suggest that early protein synthesis is not the same in abortively infected cells as it is in productively infected cells.

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1990-03-01
2022-08-13
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