1887

Abstract

The DNA of bovine adenovirus (BAV) serotype 4, a member of the subgroup 2 BA Vs, has been cloned and mapped with 11 restriction enzymes. Southern blot hybridizations probed by a clone containing about 50% of the BAV-4 genome revealed a very strong and extended DNA sequence homology amongst the members of subgroup 2, but no homology was detectable to the subgroup 1 bovine, or any of those human (HAV) and porcine adenovirus serotypes examined. These findings were strengthened by reciprocal hybridizations. When using the cloned hexon gene region of BAV-3 (subgroup 1) or the total genome of HAV-2 as probes, again no homology could be shown to the bovine subgroup 2 serotypes. The extent of DNA homology detectable between the members of bovine subgroup 1, the porcine and the human serotypes was variable, but in general less expressed than that observed within the bovine subgroup 2.

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1990-02-01
2022-08-15
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References

  1. Bartha A. 1969; Proposal for subgrouping of bovine adenoviruses. Acta veterinaria Academiae scientiarum htmgaricae 19:319–321
    [Google Scholar]
  2. BelÁk S., Berencsi GY., Rusvay M., LukÁcs K., NÁsz I. 1983; DNA structure and hemagglutination properties of bovine adeno-virus type 2 strains which bypass species specificity. Archives of Virology 77:181–194
    [Google Scholar]
  3. BelÁk S., Virtanen A., Zabielski J., Rusvai M., Berencsi G., Petterson U. 1986; Subtypes of bovine adenovirus type 2 exhibit major differences in region E3. Virology 153:262–271
    [Google Scholar]
  4. Benkö M., Bartha A., Wadell G. 1988; DNA restriction enzyme analysis of reference strains of bovine adenoviruses. Intervirology 29:346–350
    [Google Scholar]
  5. Bürki F., Hinaidy B., Bückmann J. 1979; Bovine adenoviruses. III. Subclassification into two subgroups by means of immuno-diffusion tests. Microbiologica 2:65–74
    [Google Scholar]
  6. Chang A. C. Y., Cohen S. N. 1978; Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. Journal of Bacteriology 134:1141–1156
    [Google Scholar]
  7. Clarke M. C., Sharpe H. B. A., Derbyshire J. B. 1967; Some characteristics of three porcine adenoviruses. Archiv für die gesamte Virusforschung 21:91–97
    [Google Scholar]
  8. Darbyshire J. H., Dawson P. S., Lamont R. H., Ostler D. C., Pereira G. H. 1965; A new adenovirus serotype of bovine origin. Journal of Comparative Pathology 75:327
    [Google Scholar]
  9. Hayashi K. 1980; A cloning vehicle suitable for strand separation. Gene 11:109–111
    [Google Scholar]
  10. Hu S. -L., Hays W. W., Potts D. E. 1984a; Sequence homology between bovine and human adenoviruses. Journal of Virology 49:604–608
    [Google Scholar]
  11. Hu S. -L., Battles J. K., Potts D. E. 1984b; Restriction analysis and homology studies of the bovine adenovirus 7 genome. Journal of Virology 51:880–883
    [Google Scholar]
  12. Inaba Y., Tanaka Y., Sato K., Ito H., Ito Y., Omori T., Matumoto M. 1968; Bovine adenovirus. II. A serotype, Fukuroi, recovered from Japanese cattle. Japanese Journal of Microbiology 12:219–229
    [Google Scholar]
  13. Kurokawa T., Igarashi K., Sugino Y. 1978; Biochemical studies on bovine adenovirus type 3. III. Cleavage maps of viral DNA by restriction endonucleases EcoRI, BamHI and HindIII. Journal of Virology 28:212–218
    [Google Scholar]
  14. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
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