Several dsRNA segments, ranging in size from 0·8 to 19·5 kbp, have been isolated from citrus plants infected with the VT and ST strains of citrus tristeza virus (CTV) and translated, after denaturation, in a reticulocyte lysate cell-free system. Only two small dsRNA segments of 0·8 and 1·7 kbp were efficiently translated and showed a consistent pattern of products. The major translation product of the 0·8 kbp dsRNA was a 27K polypeptide, immunoprecipitated by antiserum to the CTV coat protein. The major translation product of the 1·7 kbp dsRNA segment was a 21·5K peptide. The possibility that the CTV coat protein may be translated from a subgenomic viral messenger RNA is discussed.
AccottoG. P.,
BriscoM. J.,
HullR.1987; In vitro translation of the double-stranded RNA genome from beet cryptic virus. Journal of General Virology 68:1417–1422
Bar-JosephM.,
GarnseyS. M.,
GonsalvesD.,
MoscovitzM.,
PurcifullD. E.,
ClarkM. F.,
LoebensteinG.1979; The use of enzyme-linked immunosorbent assay for detection of citrus tristeza virus. Phytopathology 69:190–194
Bar-JosephM.,
RosnerA.,
MoskovitzM.,
HullR.1983; A simple procedure for the extraction of double-stranded RNA from viral infected plants. Journal of Virological Methods 6:1–8
BozarthR. F.,
HarleyE. H.1976; The electrophoretic mobility of dsRNA in polyacrylamide gels as a function of molecular weight. Biochimica et biophysicaacta 432:329–335
DawsonW. O.,
DoddsJ. A.1982; Characterization of subgenomic double-stranded RNAs from virus-infected plants. Biochemical and Biophysical Research Communications 107:1230–1235
GhabrialS. A.,
BibbJ. A.,
PriceK. H.,
HavensW. M.,
LesnawJ. A.1987; The capsid polypeptides of the 190S virus of Helminthosporium victoriae
. Journal of General Virology 68:1791–1800
HansenJ. N.1981; Use of solubilizable acrylamide gels for isolation of DNA fragments suitable for sequence analysis. Analytical Biochemistry 116:146–151
HiebertE.,
DoughertyW. G.1988; Organization and expression of the viral genomes. In Plant Viruses4 The Filamentous Viruses pp. 159–174MilneR. G.
Edited by New York: Plenum Press;
KaperJ. M.,
Diaz-RuizJ. R.1977; Molecular weights of the double-stranded RNAs of cucumber mosaic virus strain S and its associated RNA 5. Virology 80:214–217
KarasevA. V.,
AgranovskyA. A.,
RogovV. V.,
MiroshnichenkoN. A.,
DoljaV. V.,
AtabekovJ. G.1989; Virion RNA of beet yellows closterovirus: cell-free translation and some properties. Journal of General Virology 70:241–245
RosnerA.,
LeeR. F.,
Bar-JosephM.1986; Detection of A Florida isolate of citrus tristeza virus by differential hybridization with cloned cDNA sequences. Phytopathology 76:820–824
ShelbournS. L.,
DayP. R.,
BuckK. W.1988; Relationships and functions of virus double-stranded RNA in a P4 killer strain of Ustilago maydis
. Journal of General Virology 69:975–982
SmithM. L.,
LazdinsI.,
HolmesI. H.1980; Coding assignments of double-stranded RNA segments of SA 11 rotavirus established by in vitro translation. Journal of Virology 33:976–982