Several dsRNA segments, ranging in size from 0⋅8 to 19⋅5 kbp, have been isolated from citrus plants infected with the VT and ST strains of citrus tristeza virus (CTV) and translated, after denaturation, in a reticulocyte lysate cell-free system. Only two small dsRNA segments of 0⋅8 and 1⋅7 kbp were efficiently translated and showed a consistent pattern of products. The major translation product of the 0⋅8 kbp dsRNA was a 27K polypeptide, immunoprecipitated by antiserum to the CTV coat protein. The major translation product of the 1⋅7 kbp dsRNA segment was a 21⋅5K peptide. The possibility that the CTV coat protein may be translated from a subgenomic viral messenger RNA is discussed.


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