The nucleotide sequence of a naturally occurring satellite RNA (satRNA) of cucumber mosaic virus strain F (CMV-F) isolated from Petasites japonicum Miq. has been determined. F-satRNA is 338 nucleotides long and possesses a single open reading frame (ORF; residues 11 to 91) in its 5′-terminal region. Analysis of in vitro translation products of the RNA revealed that the major peptide synthesized had an Mt of 2800, corresponding to the value (Mr 2874) calculated from the amino acid sequence predicted from the ORF. Furthermore, a ribosome-binding fragment of the RNA in the initiation step of an in vitro translation system was found to contain the first AUG codon (residues 11 to 13). These results suggest that of the F-satRNA sequence, the coding region for the peptide can be assigned to the region from AUG to GGU (residues 11 to 91), accompanied by a termination codon UGA.
Avila-RinconM. J.,
CollmerC. W.,
KaperJ. M.1986a; In vitro translation of cucumoviral satellites. I. Purification and nucleotide sequence of cucumber mosaic virus-associated RNA 5 from cucumber mosaic virus strain S. Virology 152:446–454
Avila-RinconM. J.,
CollmerC. W.,
KaperJ. M.1986b; Cucumber mosaic virus-associated RNA 5. II. CARNA 5 from cucumber mosaic virus strain S and SP6 transcripts of cloned (S)CARNA 5 cDNA produced electrophoretically comigrating protein products. Virology 152:455–458
CollmerC. W.,
KaperJ. M.1988; Site-directed mutagenesis of potential protein-coding regions in expressible cloned cDNAs of cucumber mosaic viral satellites. Virology 163:293–298
CollmerC. W.,
TousignantM. E.,
KaperJ. M.1983; Cucumber mosaic virus-associated RNA. X. The complete nucleotide sequence of a CARNA 5 incapable of inducing tomato necrosis. Virology 127:230–234
Garcia-ArenalF.,
ZaitlinM.,
PalukaitisP.1987; Nucleotide sequence analysis of six satellite RNAs of cucumber mosaic virus: primary sequence and secondary structure alterations do not correlate with differences in pathogenicity. Virology 158:339–349
GordonK. H. J.,
SymonsR. H.1983; Satellite RNA of cucumber mosaic virus forms a secondary structure with partial 3′-terminal homology to genomal RNAs. Nucleic Acids Research 11:947–960
HemmerO.,
MeyerM.,
GriefC.,
FritschC.1987; Comparison of the nucleotide sequences of five tomato black ring virus satellite RNAs. Journal of General Virology 68:1823–1833
HidakaS.,
HanadaK.,
IshikawaK.,
MiuraK.1988; Complete nucleotide sequence of two new satellite RNAs associated with cucumber mosaic virus. Virology 164:326–333
JacquemondM.,
LauquinG. J. -M.1988; The cDNA of cucumber mosaic virus-associated satellite RNA has in vitro biological properties. Biochemical and Biophysical Research Communications 151:388–395
KaperJ. M.,
DuriatA. S.,
TousignantM. E.1986; The 368- nucleotide satellite of cucumber mosaic virus strain Y from Japan does not cause lethal necrosis in tomato. Journal of General Virology 67:2241–2246
KaperJ. M.,
TousignantM. E.,
SteenM. T.1988; Cucumber mosaic virus-associated RNA 5. XI. Comparison of 14 CARNA 5 variants relates ability to induce tomato necrosis to a conserved nucleotide sequence. Virology 163:284–292
KurathG.,
PalukaitisP.1987; Biological activity of T7 transcripts of a prototype clone and a sequence variant clone of a satellite RNA of cucumber mosaic virus. Virology 159:199–208
YamaguchiK.,
HidakaS.,
MiuraK.1982; Relationship between structure of the 5′ non-coding region of viral mRNA and efficiency in the initiation step of protein synthesis in a eukaryotic system. Proceedings of the National Academy of Sciences, U.S.A 79:1012–1016