@article{mbs:/content/journal/jgv/10.1099/0022-1317-71-2-433, author = "Accotto, G. P. and MarzachÍ, C. and Luisoni, E. and Milne, R. G.", title = "Molecular characterization of alfalfa cryptic virus 1", journal= "Journal of General Virology", year = "1990", volume = "71", number = "2", pages = "433-437", doi = "https://doi.org/10.1099/0022-1317-71-2-433", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-71-2-433", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Alfalfa cryptic virus 1 (ACV1) was purified from alfalfa plant clone (Medicago sativa) and characterized. The genome of ACV 1 consists of two dsRNAs, one with an estimated Mr of 1·27 × 106 (RNA 1) and the other of Mr 1·17 × 106 (RNA 2); the virus capsid is built from one polypeptide of estimated Mr 54000. An RNA- dependent RNA polymerase able to replicate the genomic RNAs in vitro is associated with purified virus particles. In vitro translation showed that each of the genomic RNAs encodes a polypeptide and that encoded by RNA 2 is the capsid protein. These polypeptides account for about 95 % and 83 % of the coding capacity of RNA 1 and RNA 2, respectively. In Western and Northern blot experiments close affinity was found between ACV 1 and hop trefoil cryptic virus 1, a cryptovirus found in Medicago lupulina. No affinity was detected with any other cryptovirus tested.", }