1887

Abstract

Alfalfa cryptic virus 1 (ACV1) was purified from alfalfa plant clone () and characterized. The genome of ACV 1 consists of two dsRNAs, one with an estimated of 1·27 × 10 (RNA 1) and the other of 1·17 × 10 (RNA 2); the virus capsid is built from one polypeptide of estimated 54000. An RNA- dependent RNA polymerase able to replicate the genomic RNAs is associated with purified virus particles. translation showed that each of the genomic RNAs encodes a polypeptide and that encoded by RNA 2 is the capsid protein. These polypeptides account for about 95 % and 83 % of the coding capacity of RNA 1 and RNA 2, respectively. In Western and Northern blot experiments close affinity was found between ACV 1 and hop trefoil cryptic virus 1, a cryptovirus found in . No affinity was detected with any other cryptovirus tested.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-2-433
1990-02-01
2022-09-30
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/2/JV0710020433.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-2-433&mimeType=html&fmt=ahah

References

  1. Accotto G. P., Brisco M. J., Hull R. 1987; In vitro translation of the double-stranded RNA genome from beet cryptic virus 1. Journal of General Virology 68:1417–1422
    [Google Scholar]
  2. Blake M. S., Johnston K. H., Russell-Jones G. J., Gotschlich E. C. 1984; A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots. Analytical Biochemistry 136:175–179
    [Google Scholar]
  3. Boccardo G., Accotto G. P. 1988; RNA-dependent RNA polymerase activity in two morphologically different white clover cryptic viruses. Virology 163:413–419
    [Google Scholar]
  4. Boccardo G., Milne R. G., Luisoni E., Lisa V., Accotto G. P. 1985; Three seedborne cryptic viruses containing double-stranded RNA isolated from white clover. Virology 147:29–40
    [Google Scholar]
  5. Boccardo G., Lisa V., Luisoni E., Milne R. G. 1987; Cryptic plant viruses. Advances in Virus Research 32:171–213
    [Google Scholar]
  6. Davies J. W. 1979; Translation of plant virus nucleic acids in extracts from euearyotic cells. In Nucleic Acids in Plants 2 pp. 114–149 Hall T. C., Davies J. W. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  7. Dretzen G., Bellard M., Sassone-Corsi P., Chambon P. 1981; A reliable method for the recovery of DNA fragments from agarose and acrylamide gels. Analytical Biochemistry 112:295–298
    [Google Scholar]
  8. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  9. Lisa V., Luisoni E., Milne R. G. 1981; A possible virus cryptic in carnation. Annals of Applied Biology 98:431–437
    [Google Scholar]
  10. Luisoni E., Milne R. G. 1988; White clover cryptic virus 2. AAB Description of Plant Viruses332
    [Google Scholar]
  11. Luisoni E., Milne R. G., Accotto G. P., Boccardo G. 1987; Cryptic viruses in hop trefoil (Medicago lupulina) and their relationships to other cryptic viruses in legumes. Intervirology 28:144–156
    [Google Scholar]
  12. McMaster G. K., Carmichael G. G. 1977; Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proceedings of the National Academy of Sciences, U.S.A 74:4835–4838
    [Google Scholar]
  13. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  14. Marzachi C., Milne R. G., Boccardo G. 1988; In vitro synthesis of double-stranded RNA by carnation cryptic virus-associated RNA-dependent RNA polymerase. Virology 165:115–121
    [Google Scholar]
  15. Morris T. J., Dodds J. A. 1979; Isolation and analysis of doublestranded RNA from virus-infected plant and fungal tissue. Phyto-pathology 69:854–858
    [Google Scholar]
  16. Natsuaki T., Natsuaki K. T., Okuda S., Teranaka M., Milne R. G., Boccardo G., Luisoni E. 1986; Relationships between the cryptic and temperate viruses of alfalfa, beet and white clover. Intervirology 25:69–75
    [Google Scholar]
  17. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A 76:4350–4354
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-2-433
Loading
/content/journal/jgv/10.1099/0022-1317-71-2-433
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error