@article{mbs:/content/journal/jgv/10.1099/0022-1317-71-2-411, author = "Fenwick, M. L. and Everett, R. D.", title = "Transfer of UL41, the gene controlling virion-associated host cell shutoff, between different strains of herpes simplex virus", journal= "Journal of General Virology", year = "1990", volume = "71", number = "2", pages = "411-418", doi = "https://doi.org/10.1099/0022-1317-71-2-411", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-71-2-411", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Studies with mutant viruses have suggested that the product of gene UL41 of herpes simplex virus type 1 (HSV-1) controls the virion-mediated inhibition of cellular protein synthesis as well as the rate of degradation of viral mRNAs. HSV-1 strain 17+ has a weak host shutoff function, whereas HSV-2 strain G shuts off strongly. A gene of HSV-2(G), judged from its position in the genome to be the probable analogue of gene UL41 of HSV-1, was inserted into the nonessential thymidine kinase gene of HSV-1(17+). The recombinant virus, 17G41, exhibited a strong shutoff function and its immediate early mRNA did not accumulate in the presence of cycloheximide. It resembled HSV-2(G) in these respects and not the parent, confirming the function of the transferred gene. Recombinant virus 17G41 carries the UL41 genes of both strains, 17+ and G, and in this situation the strong shutoff function was dominant. However, after mixed infection with equal multiplicities of 17G41 and HSV- 1(17+) the weak shutoff function was dominant. The recombinant, 17G41, was further modified by insertion of a lacZ expression cassette into the coding region of the original gene UL41 (17+). The resulting virus, 17(41−)G41, also had a strong shutoff activity but grew poorly in tissue culture.", }