1887

Abstract

Freshly isolated, and established, cultures of bovine peripheral blood mononuclear leukocytes (PBMLs) were exposed to bluetongue virus (BTV) for the purpose of defining potential lymphotropism. PBML cultures were established in the presence of interleukin 2 (IL-2) and mitogen and maintained either as bulk culture or were cloned prior to infectivity studies. All cultures appeared to be of the T cell phenotype based on the following characteristics: binding of T lymphocyte-specific lectins (i.e. peanut agglutinin and , rosetting of sheep erythrocytes, binding of a putative pan-T monoclonal antibody, and absence of surface immunoglobulin (Ig). T lymphocyte cultures were further characterized by their ability to elicit lectin-dependent cellular cytotoxicity (LDCC). Exposure of established lymphocyte cultures to BTV resulted in productive cytopathic and non-cytopathic infections. Non-cytopathic productive infections were observed in LDCC-negative cultures whereas cytopathic and non-cytopathic infections were observed in LDCC-positive cultures. Exposure of freshly isolated PBMLs to BTV resulted in minimal virus replication; addition of mitogen and IL-2 to such cultures did not augment virus replication. Addition of mitogen and IL- 2 induced negligible blast transformation, whereas PBML viability was minimally affected. These studies establish a tropism of BTV for bovine T lymphocytes with virus replication being limited to those cells undergoing blastogenesis. Establishment of infected lymphocyte cultures, without loss of culture viability, suggest such an interaction may contribute to the long term viraemias associated with BTV infection of cattle.

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1990-02-01
2023-02-05
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References

  1. Collisson E. W., Barber T. L. 1983; Blood cells associated with bluetongue virus infection in cattle. American Association of Veterinary Laboratory Diagnosticians, Proceedings of the Annual Meeting 26:287–300
    [Google Scholar]
  2. DuToit R. M. 1962; The role played by bovines in the transmission of bluetongue in sheep. Journal of the South African Veterinary Medical Association 33:483–490
    [Google Scholar]
  3. Heidner H. W., Maclachlan N. J., Fuller F. J., Richards R. G., Whetter L. E. 1988; Bluetongue virus genome remains stable throughout prolonged infection of cattle. Journal of General Virology 69:2629–2636
    [Google Scholar]
  4. Howell P. G., Verwoerd D. W., Oellermann R. A. 1967; Plaque formation by bluetongue virus. Onderstepoort Journal of Veterinary Research 34:317–332
    [Google Scholar]
  5. Johansson C., Morein B. 1983; Evaluation of labeling methods for bovine T and B lymphocytes. Veterinary Immunology and Immunopathology 4:345–359
    [Google Scholar]
  6. Luedke A. J. 1970; Distribution of virus in blood components during viremia of bluetongue. United States Animal Health Association, Proceedings of the Annual Meeting 74:9–21
    [Google Scholar]
  7. Luedke A. J., Jochim M. M., Jones R. H. 1969; Bluetongue in cattle: viremia. American Journal of Veterinary Researches 30:511–516
    [Google Scholar]
  8. Maclachlan N. J., Fuller F. J. 1986; Genetic stability in calves of a single strain of bluetongue virus. American Journal of Veterinary Research 47:762–764
    [Google Scholar]
  9. Maclachlan N. J., Heidner H. W., Fuller F. J. 1987; Humoral immune response of calves to bluetongue virus infection. American Journal of Veterinary Research 48:1031–1035
    [Google Scholar]
  10. Morein B., Hellstrom U., Axelsson L. G., Johansson L. C., Hammarstrom S. 1979; Helix pomatia A hemagglutinin, a surface marker for bovine T lymphocytes. Veterinary Immunology and Immunopathology 1:27–36
    [Google Scholar]
  11. Morrill J. C., McConnell S. 1985; An electron microscopic study of blood cells from calves experimentally infected with bluetongue virus. In Bluetongue and Related Orbiviruses pp. 279–287 Barber T. L., Jochim M. M. Edited by New York; Alan R. Liss:
    [Google Scholar]
  12. Parsonson I. M., Della-Porta A. J., McPhee D. A., Cybinski D. H., Squire K. R. E., Uren M. F. 1987; Experimental infection of bulls and cows with bluetongue virus serotype 20. Australian Veterinary Journal 64:10–13
    [Google Scholar]
  13. Paul P. S., Brown T. T., Miller J. M., Van Der Maaten M. J. 1979; Enhancement of rosette formation between sheep erythrocytes and bovine T-lymphocytes by 2-aminoethylisothiouronium bromide and dextran. Immunology Letters 1:93–96
    [Google Scholar]
  14. Pearson T. W., Roelants G. E., Lundin L. B., Mayor-Withey K. S. 1979; The bovine lymphoid system. Binding and stimulation of peripheral blood lymphocytes by lectins. Journal of Immunological Methods 26:271–282
    [Google Scholar]
  15. Richards R. G., MacLachlan N. J., Heidner H. W., Fuller F. J. 1988; Comparison of virologic and serologic responses of lambs and calves infected with bluetongue virus serotype 10. Veterinary Microbiology 18:233–242
    [Google Scholar]
  16. Scibienski R. J., Stott M. L., Stott J. L. 1987; Establishment and initial characterization of continuous in vitro cultures of bovine T lymphocytes. Veterinary Immunology and Immunopathology 14:209–222
    [Google Scholar]
  17. Stott J. L., Barber T. L., Osburn B. I. 1978; Serotyping bluetongue virus: a comparison of plaque inhibition (disc) and plaque neutralization methods. American Association of Veterinary Laboratory Diagnosticians, Proceedings of the Annual Meeting 21:399–410
    [Google Scholar]
  18. Theilen G. H., Miller J. M., Higgins J., Ruppanner R. N., Garrett W. 1982; Vaccination against bovine leukaemia virus infection. In Fourth International Symposium on Bovine Leukosis pp. 547–559 Straub O. C. Edited by The Hague: Martinus Nijoff;
    [Google Scholar]
  19. Whetter L. E., MacLachlan N. J., Gebhard D. H., Heidner H. W., Moore P. F. 1989; Bluetongue virus infection of bovine monocytes. Journal of General Virology 70:1663–1676
    [Google Scholar]
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