1887

Abstract

The cDNA sequence of the large dsRNA segment (segment A) of the N1 strain of infectious pancreatic necrosis virus (IPNV) has been determined. The nucleotide and deduced amino acid sequences were compared to the sequences of segment A of the Jasper strain of IPNV and to the sequences of segments A and B (5′ and 3′ flanking regions) of the 002–73 strain of infectious bursal disease virus (IBDV). The comparison demonstrated that the precursor protein of the major structural polypeptide, pVP2, is highly conserved at the N and C termini, whereas the amino acid sequence of an internal segment shows greater diversity between the strains. This internal segment probably carries the serotype-specific epitopes of birnaviruses. An alternative open reading frame (ORF) (444 bp) partly overlapping with the large ORF (2916 bp) of segment A was found to be conserved among the IPNV strains and is probably also present in the 002–73 strain of IBDV. This small ORF may encode a novel birnavirus polypeptide with an of 17K. SDS-PAGE of radiolabelled purified IPNV particles revealed a band corresponding to the possible novel 17K polypeptide. Short terminal inverted repeats are found in segment A of the N1 and Jasper strains of IPNV and in segment B of the 002–73 strain of IBDV. Segment A of IPNV and segment B of IBDV also contain adjacent inverted repeats at their 5′-terminal flanking regions.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-2-299
1990-02-01
2024-12-14
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/2/JV0710020299.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-2-299&mimeType=html&fmt=ahah

References

  1. Azad A. A., Barrett S. A., Fahey K. J. 1985; The characterization and molecular cloning of the double-stranded RNA genome of an Australian strain of infectious bursal disease virus. Virology 143:35–44
    [Google Scholar]
  2. Azad A. A., Jagadish M. N., Brown M. A., Hudson P. J. 1987; Deletion mapping and expression in Escherichia coliof the large genomic segment of birnavirus. Virology 161:145–152
    [Google Scholar]
  3. Becht H., Müller H., Müller H. K. 1988; Comparative studies on structural and antigenic properties of two serotypes of infectious bursal disease virus. Journal of General Virology 69:631–640
    [Google Scholar]
  4. Bernard J. 1980; Drosophila X virus RNA polymerase: tentative model for in vitroreplication of the double-stranded virion RNA. Journal of Virology 33:717–723
    [Google Scholar]
  5. Caswell-Reno P., Reno P. W., Nicholson B. L. 1986; Monoclonal antibodies to infectious pancreatic necrosis virus: analysis of viral epitopes and comparison of different isolates. Journal of General Virology 67:2193–2205
    [Google Scholar]
  6. Cheville N. F. 1967; Studies on the pathogenesis of Gumboro disease in the bursa of Fabricius, spleen and thymus of the chicken. American Journal of Pathology 51:527–551
    [Google Scholar]
  7. Christie K. E., Håvarstein L. S. 1988; A new serotype of infectious necrosis virus (IPN Nl). International symposium on viruses of lower vertebrates. Series of Life Sciences in press
    [Google Scholar]
  8. Christie K. E., Håvarstein L. S., Djupvik H. O., Ness S., Endresen C. 1988; Characterization of a new serotype of infectious necrosis virus isolated from Atlantic salmon. Archives of Virology 103:167–177
    [Google Scholar]
  9. Dayhoff M. 1978 Atlas of Protein Sequence and Structure 5 supplement 3 Washington, D.C.: National Biomedical Research Foundation;
    [Google Scholar]
  10. Dobos P., Roberts T. E. 1983; The molecular biology of infectious necrosis virus: a review. Canadian Journal of Microbiology 29:377–384
    [Google Scholar]
  11. Dobos P., Hill B. J., Hallett R., Kells D. T. C., Becht H., Teninges D. 1979; Biophysical and biochemical characterization of five animal viruses with bisegmented dsRNA genomes. Journal of Virology 32:593–605
    [Google Scholar]
  12. Duncan R., Dobos P. 1986; The nucleotide sequence of infectious pancreatic necrosis virus (IPNV) dsRNA segment A reveals one large ORF encoding a precursor polyprotein. Nucleic Acids Research 14:5934
    [Google Scholar]
  13. Duncan R., Nagy E., Krell P. J., Dobos P. 1987; Synthesis of the infectious pancreatic necrosis virus polyprotein, detection of a virus-encoded protease, and fine structure mapping of genome segment A coding regions. Journal of Virology 61:3655–3664
    [Google Scholar]
  14. Fahey K. J., O’Donnell I. J., Azad A. A. 1985; Characterization by Western blotting of the immunogens of infectious bursal disease virus. Journal of General Virology 66:1479–1488
    [Google Scholar]
  15. Gorbalenya A. E., Koonin E. V. 1988; Birnavirus RNA polymerase is related to polymerases of positive strand RNA viruses. Nucleic Acids Research 16:77–35
    [Google Scholar]
  16. Hill B. J. 1982; Infectious pancreatic necrosis virus and its virulence. In Microbial Diseases of Fish pp 91–114 Roberts R. J. Edited by New York & London: Academic Press;
    [Google Scholar]
  17. Hudson P. J., Mckern N. M., Power B. E., Azad A. A. 1986; Genomic structure of the large RNA segment of infectious bursal disease virus. Nucleic Acids Research 14:5001–5012
    [Google Scholar]
  18. Jagadish M. N., Staton V. J., Hudson P. J., Azad A. A. 1988; Birnavirus precursor polyprotein is processed in Escherichia coliby its own virus-encoded polypeptide. Journal of Virology 62:1084–1087
    [Google Scholar]
  19. Kibenge F. S. B., Dhillon A. S., Russell R. G. 1988; Biochemistry and immunology of infectious bursal disease virus. Journal of General Virology 69:1757–1775
    [Google Scholar]
  20. Kozak M. 1986; Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eucaryotic ribosomes. Cell 44:283–292
    [Google Scholar]
  21. Kozak M. 1988; Leader length and secondary structure modulate mRNA function under conditions of stress. Molecular and Cellular Biology 8:2737–2744
    [Google Scholar]
  22. Kyte J., Doolittle R. F. 1982; A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology 157:105–132
    [Google Scholar]
  23. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  24. Mertens P. P. C., Jamieson P. B., Dobos P. 1982; In vitroRNA synthesis by infectious pancreatic necrosis virus-associated RNA polymerase. Journal of General Virology 59:47–56
    [Google Scholar]
  25. Morgan M. M., Macreadie I. G., Harley V. R., Hudson P. J., Azad A. A. 1988; Sequence of the small double-stranded RNA genomic segment of infectious bursal disease virus and its deduced 90-kDa product. Virology 163:240–242
    [Google Scholar]
  26. Müller H., Becht H. 1982; Biosynthesis of virus-specific proteins in cells infected with infectious bursal disease virus and their significance as structural elements for infectious virus and incomplete particles. Journal of Virology 44:384–392
    [Google Scholar]
  27. Müller H., Scholtissek C., Becht H. 1979; The genome of infectious bursal disease virus consists of two segments of double stranded RNA. Journal of Virology 31:584–589
    [Google Scholar]
  28. Nagy E., Duncan R., Krell P., Dobos P. 1987; Mapping of the large genome segment of infectious pancreatic necrosis virus by hybrid arrested translation. Virology 158:211–217
    [Google Scholar]
  29. Pelletier J., Sonnenberg N. 1985; Insertion mutagenesis to increase secondary structure within the 5ʹ noncoding region of a eukaryotic mRNA reduces translational efficiency. Cell 40:515–526
    [Google Scholar]
  30. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences U.S.A.: 745463–5467
    [Google Scholar]
  31. Spies U., Müller H., Becht H. 1987; Properties of RNA polymerase activity associated with infectious bursal disease virus and characterization of its reaction products. Virus Research 8:127–140
    [Google Scholar]
  32. Teninges D., Ohanessian A., Richard-Molard C., Contamine D. 1979; Isolation and biological properties of DrosophilaX virus. Journal of General Virology 42:241–254
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-71-2-299
Loading
/content/journal/jgv/10.1099/0022-1317-71-2-299
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error