1887

Abstract

The complete nucleotide sequence of segment 5 (S5) of the rice dwarf virus (RDV) genome was determined. RD V S5 is 2571 bp in length and has a single long open reading frame which encodes a polypeptide of 801 amino acids ( 90495). When compared to the wound tumor virus genome S5, there was 56·9% and 52·8% similarity in the nucleotide and amino acid sequences, respectively. This high similarity suggests that the S5 proteins of these viruses are functionally similar.

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1990-12-01
2024-12-08
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References

  1. Anzola J. V., Xu Z., Asamizu T., Nuss D. L. 1987; Segment-specific inverted repeats found adjacent to conserved terminal sequences in wound tumor virus genome and defective interfering RNAs. Proceedings of the National Academy of Sciences, U.S.A 84:8301–8305
    [Google Scholar]
  2. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  3. Black L. M. 1970; Wound tumor virus. CMI/AAB Descriptions of Plant Viruses34
    [Google Scholar]
  4. Boccardo G., Milne R. G. 1984; Plant reovirus group. CMI/AAB Descriptions of Plant Viruses294
    [Google Scholar]
  5. Cashdollar L. W., Esparza J., Hudson G. R., Chmelo R., Lee P. W. K., Joklik W. K. 1982; Cloning the double-stranded RNA genes of reovirus: sequence of the cloned S2 gene. Proceedings of the National Academy of Sciences, U.S.A 79:7644–7648
    [Google Scholar]
  6. Dayhoff M. O., Eck R. V., Park C. M. 1972; A model of evolutionary change in proteins. In Atlas of Protein Sequence and Structure 5 pp. 89–99 Dayhoff M. O. Edited by Washington, D.C: National Biomedical Research Foundation;
    [Google Scholar]
  7. Fujii-Kawata I., Miura K., Fuke M. 1970; Segments of genomes of viruses containing double-stranded ribonucleic acid. Journal of Molecular Biology 51:247–253
    [Google Scholar]
  8. Iida T. T., Shinkai A., Kimura I. 1972; Rice dwarf virus. CMI/AAB Descriptions of Plant Viruses102
    [Google Scholar]
  9. Kimura I., Minobe Y., Omura T. 1987; Changes in a nucleic acid and a protein component of rice dwarf virus particles associated with an increase in symptom severity. Journal of General Virology 68:3211–3215
    [Google Scholar]
  10. Kodama T., Suzuki N. 1973; RNA polymerase activity in purified rice dwarf virus. Annals of the Phytopathological Society of Japan 39:251–258
    [Google Scholar]
  11. Kozak M. 1987; An analysis of 5′-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Research 15:8125–8148
    [Google Scholar]
  12. Messing J., Crea R., Seeburg P. H. 1981; A system for shotgun DNA sequencing. Nucleic Acids Research 9:309–321
    [Google Scholar]
  13. Minobe Y. 1987; Molecular cloning and characterization of the genome of rice dwarf virus. Annual Report of the National Institute of Agrobiological Resources 3:34–38
    [Google Scholar]
  14. Mizusawa S., Nishimura S., Seela F. 1986; Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP. Nucleic Acids Research 14:1319–1324
    [Google Scholar]
  15. Nakashima K., Kakutani T., Minobe Y. 1990; Sequence analysis and product assignment of segment 7 of the rice dwarf virus genome. Journal of General Virology 71:725–729
    [Google Scholar]
  16. Nakata M., Fukunaga K., Suzuki N. 1978; Polypeptide components of rice dwarf virus. Annals of the Phytopathological Society of Japan 44:288–296
    [Google Scholar]
  17. Omura T., Inoue H. 1985; Rice gall dwarf virus. CMI/AAB Descriptions of Plant Viruses296
    [Google Scholar]
  18. Omura T., Morinaka T., Inoue H., Saito Y. 1982; Purification and some properties of rice gall dwarf virus, a new phytoreovirus. Phytopathology 72:1246–1249
    [Google Scholar]
  19. Omura T., Minobe Y., Tsuchizaki T. 1988; Nucleotide sequence of segment S10 of the rice dwarf virus genome. Journal of General Virology 69:227–231
    [Google Scholar]
  20. Omura T., Ishikawa K., Hirano H., Ugaki M., Minobe Y., Tsuchizaki T., Kato H. 1989; The outer capsid protein of rice dwarf virus is encoded by genome segment S8. Journal of General Virology 70:2759–2764
    [Google Scholar]
  21. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  22. Suzuki N., Watanabe Y., Kusano T., Kitagawa Y. 1989; Nucleotide sequence of rice dwarf virus segment 5. Nucleic Acids Reienrrh 17:8858
    [Google Scholar]
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