1887

Abstract

Human placental trophoblast interferon (tro-IFN), induced in trophoblast cultures by a superinduction procedure, was purified to a homogeneous product with retention of biological activity. The problems associated with isolation from serum-containing medium were overcome by a combination of Blue Sepharose affinity chromatography and reversed-phase HPLC (RP-HPLC) on Separon SGX C-18. This two-step purification procedure yielded tro-IFN with a specific activity of 3·4 × 10 international units/mg of protein. The overall recovery of interferon activity was 66·7%. The purified tro-IFN was shown to be a glycoprotein with an of 24K on native and SDS-PAGE. Its antiviral activity was stable at pH 2·0 at 37 °C but was sensitive to heat at 56 °C for 1 h and was neutralized by antibodies to human IFN-.

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1990-12-01
2022-10-07
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