@article{mbs:/content/journal/jgv/10.1099/0022-1317-71-12-2953, author = "McLean, Gordon and Rixon, Frazer and Langeland, Nina and Haarr, Lars and Marsden, Howard", title = "Identification and Characterization of the Virion Protein Products of Herpes Simplex Virus Type 1 Gene UL47", journal= "Journal of General Virology", year = "1990", volume = "71", number = "12", pages = "2953-2960", doi = "https://doi.org/10.1099/0022-1317-71-12-2953", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-71-12-2953", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "We have identified two encoded proteins with an antiserum raised against a synthetic oligopeptide corresponding to amino acids 671 to 684 of the predicted protein product of gene UL47 of herpes simplex virus type 1 (HSV-1). They have apparent Mrs of 82000 and 81000 and are both major virion components located in the tegument. The 82/8IK proteins were first detected in infected cells in minor amounts 6 h after infection at 37 °C but were later (from 10 h until 24 h after infection) present in large amounts. UL47 regulation was investigated using phosphonoacetic acid (PAA), an inhibitor of DNA synthesis: the amounts of the 82/8IK protein synthesized were compared with those of 65KDBP, an early gene product, and 21K/22K, a true late gene product. The data showed that UL47 is regulated as a true late gene.", }