The nucleotide sequence of an unusual fowlpox virus gene which maps immediately upstream from the fowlpox virus 4b gene has been determined. The 34000 Mr protein predicted to be encoded by the gene contains 11 copies of a 12 amino acid serine-rich repeat sequence. The seven amino-terminal copies of the repeat sequence are perfectly conserved but variation exists in the four carboxy-terminal copies. Three peptides were synthesized which contained either one copy of the repeat sequence, two copies of the repeat sequence or a hydrophilic amino-terminal region of the protein. All three peptides when injected with adjuvant into rabbits gave rise to antibodies which reacted strongly on Western blots of purified fowlpox virus proteins with a 39000 Mr protein. When directly compared in Western blots the antipeptide sera were shown to recognize a protein comigrating with one of the two immunodominant proteins recognized by chicken anti-fowlpox virus sera taken 2 weeks postinfection. The virion protein is removed by treatment with sodium deoxycholate suggesting that it is located at or near the surface of the virus.
BigginM. D.,
GibsonT. J.,
HongG. F.1983; Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proceedings of the National Academy of Sciences U.S.A.: 803963–3965
BinnsM. M.,
BoursnellM. E. G.,
TomleyF. M.,
MockettA. P. A.,
CavanaghD.1986; Prospects for a novel genetically engineered vaccine against infectious bronchitis. Israel Journal of Veterinary Medicine 42:124–127
BinnsM. M.,
BoursnellM. E. G.,
TomleyF. M.,
CampbellJ.1989; Analysis of the fowlpox virus gene encoding the 4b core polypeptide and demonstration that it possesses efficient promoter sequences. Virology 170:288–291
KinchingtonP. R.,
RemenickJ.,
OstroveJ. M.,
StrausS. E.,
RuyechanW. T.,
HayJ.1986; Putative glycoprotein gene of varicella-zoster virus with variable copy numbers of a 42-base-pair repeat sequence has homology to herpes simplex virus glycoprotein C. Journal of Virology 59:660–668
MockettA. P. A.,
SoutheeD. J.,
TomleyF. M.,
DeuterA.1987; Fowlpox virus: its structural polypeptides and immunogens and the detection of viral-specific antibodies by ELISA. Avian Pathology 16:493–504
RoselJ.,
MossB.1985; Transcriptional and translational mapping and nucleotide sequence analysis of a vaccinia virus gene encoding the precursor of the major core polypeptide 4b. Journal of Virology 56:830–838
SangerF.,
NicklenS.,
CoulsonA. R.1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences U.S.A.: 745463–5467
StadenR.1982; Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucleic Acids Research 10:4731–4751