1887

Journal of General Virology header logo

Volume 71, Issue 12

Theiler’s Murine Encephalomyelitis Virus-binding Activity on Neural and Non-neural Cell Lines and Tissues Free

Abstract

Three categories of cell lines are described with respect to their activity in binding Theiler ’s murine encephalomyelitis virus (TMEV). High, medium and low densities of viral receptors can be detected on cell lines from different species and origins by using an immunological binding assay. Nevertheless, TMEV acts as a fastidious virus that only infects a few cell types productively. No correlation between virion binding and degree of permissiveness to infection could be detected. The presence of viral receptors in both susceptible and resistant strains of mice seemed to have a widespread tissue distribution, the thymus being an exception. When primary cerebral cultures, enriched in neurons, astrocytes or oligodendrocytes, were checked in the immunological assay, a higher density of viral receptors was detected in the neuronal population. The number of virus-binding sites in the BHK-21 cell line is reported here to be 5 × 10 per cell; approximately 15 × 10 and 2·5 × 10 are the estimates of binding sites per cultured neuron and macroglial cell, respectively.

  • Received:
  • Accepted:
  • Published Online:
© Society for General Microbiology 1990
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-12-2867
1990-12-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/12/JV0710122867.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-12-2867&mimeType=html&fmt=ahah

References

  1. Bukrinskaya A. G. 1982; Penetration of viral genetic material into host cell. Advances in Virus Research 27:141–204
     [Google Scholar]
  2. Dal Canto M. C., Lipton H. L. 1982; Ultrastructural immunohistochemical localization of virus in acute and chronic demyelination Theilerʹs virus infection. American Journal of Pathology 106:20–29
     [Google Scholar]
  3. Dimmock N. J. 1982; Initial stages in infection with animal viruses. Journal of General Virology 59:1–22
     [Google Scholar]
  4. Gearing A. J. H., Johnstone A. P., Thorpe R. 1985; Production and assay of the interleukins. Journal of Immunological Methods 83:1–27
     [Google Scholar]
  5. Greve J. M., Davis G., Meyer A. M., Forte C. P., Yost S. C., Marlow C. W., Kamarck M. E., McClelland A. 1989; The major human rhinovirus receptor is ICAM-1. Cell 556:839–847
     [Google Scholar]
  6. Jondall M., Klein G. 1983; Surface markers on human B and T lymphocytes. II. Presence of Epstein-Barr virus receptors on B lymphocytes. Journal of Experimental Medicine 138:1365–1378
     [Google Scholar]
  7. Kohn A. 1979; Early interactions of viruses with cellular membranes. Advances in Virus Research 24:223–276
     [Google Scholar]
  8. Krontikis T. G., Soeiro R., Fields R. N. 1973; Host restriction of Friend leukemia virus. Role of the viral outer coat. Proceedings of the National Academy of Sciences U.S.A.: 702549–2553
     [Google Scholar]
  9. Lipton H. L. 1975; Theiler’s virus infection in mice: an unusual biphasic disease process leading to demyelination. Infection and Immunity 11:1147–1155
     [Google Scholar]
  10. Lipton H. L., Dal Canto M. C. 1976; Theiler’s virus induced demyelination: prevention by immunosuppression. Science 192:6264
     [Google Scholar]
  11. Liu CH., Collins J., Sharp E. 1967; The pathogenesis of Theilerʹs GDVII encephalomyelitis virus infection in mice as studied by immunofluorescent technique and infectivity titrations. Journal of Immunology 98:46–55
     [Google Scholar]
  12. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
     [Google Scholar]
  13. McCarthy K. D., De Vellis J. 1980; Preparation of separate astroglia and oligodendroglial cell cultures from rat cerebral tissue. Journal of Cell Biology 85:890–902
     [Google Scholar]
  14. McLaren L. C., Holland J. J., Syverton J. T. 1959; The mammalian cell virus relationship. I. Attachment of poliovirus to cultivated cells of primate and non-primate origin. Journal of Experimental Medicine 109:475–485
     [Google Scholar]
  15. Manthorpe M., Adler M., Varon S. 1979; Development, reactivity and GFA immunofluorescence of astroglia containing monolayer cultures from rat cerebrum. Journal of Neurocytology 8:605–621
     [Google Scholar]
  16. Melvold R. W., Jokinen D. M., Knobler R. L., Lipton H. L. 1987; Variations in genetic control of susceptibility to Theilerʹs murine encephalomyelitis virus (TMEV)-induced demyelinating disease. I. Differences between susceptible SJL/J and resistant BALB/c strains map near the T cell β-chain constant gene on chromosome 6. Journal of Immunology 138:1429–1433
     [Google Scholar]
  17. Mendelsohn C. L., Wimmer E., Racaniello V. R. 1989; Cellular receptor for poliovirus: molecular cloning, nucleotide sequence, and expression of a new member of the immunoglobulin superfamily. Cell 56:855–865
     [Google Scholar]
  18. Piraino F. 1967; The mechanism of genetic resistance of chick embryo cells to infection by Rous sarcoma virus Bryan strain. Virology 32:700–707
     [Google Scholar]
  19. Ransom B. R., Neale E., Fienkart M., Bullock P. N., Nelson P. G. 1977a; Mouse spinal cord in cell culture. I. Morphology and intrinsic neuronal electrophysiologic properties. Journal of Neurophysiology 4:1132–1150
     [Google Scholar]
  20. Ransom B. R., Christian C., Bullock P. N., Nelson P. G. 1977b; Mouse spinal cord in cell culture. II. Synaptic activity and circuit behavior. Journal of Neurophysiology 40:1151–1162
     [Google Scholar]
  21. Reif E. A., Allen J. M. V. 1964; The AKR thymic antigen and its distribution in leukemias and nervous tissues. Journal of Experimental Medicine 120:413–433
     [Google Scholar]
  22. Rodriguez M., Leibowitz J. L., Powell H. C., Lampert P. W. 1983; Neonatal infection with the Daniels strain of Theilerʹs murine encephalomyelitis virus. Laboratory Investigation 33:672–679
     [Google Scholar]
  23. Rodriguez M., Siegel L. M., Havanec-Burus D., Bologna L., Graves M. C. 1988; Theilerʹs virus-associated antigens on the surfaces of cultured glial cells. Virology 166:463–474
     [Google Scholar]
  24. Rubio N., Cuesta A. 1988; Receptors for Theilerʹs murine encephalomyelitis virus: characterization by using rabbit antiviral antiserum. Journal of Virology 62:4303–4306
     [Google Scholar]
  25. Rubio N., Cuesta A. 1989; Lack of cross-reaction between myelin basic proteins and putative demyelinating virus envelope proteins. Molecular Immunology 26:663–668
     [Google Scholar]
  26. Rustigian R., Pappenheimer A. M. 1949; Myositis in mice following intramuscular infection of viruses of the mouse encephalomyelitis group and certain other neurotropic viruses. Journal of Experimental Medicine 89:69–92
     [Google Scholar]
  27. Stauton D. E., Merluzzi V. J., Rothlein R., Barton R., Martin S. D., Springer T. A. 1989; A cell adhesion molecule, ICAM-1, is the major surface receptor for rhinoviruses. Cell 56:849–853
     [Google Scholar]
  28. Steck F. T., Rubin H. 1966; The mechanism of interference between an avian leukosis and Rous sarcoma virus. II. Early steps of infection by RSV of cells under conditions of interference. Virology 60:574–582
     [Google Scholar]
  29. Stroop W. G., Baringer J. R., Brahic M. 1981; Detection of Theilerʹs virus RNA in mouse central nervous system by in situ hybridization. Laboratory Investigations 45:504–509
     [Google Scholar]
  30. Sturman L. S., Tamm I. 1966; Host dependence of GDVII virus: complete or abortive multiplication in various cell types. Journal of Immunology 97:885–896
     [Google Scholar]
  31. Tardieu M., Boespflug O., Barbe T. 1986; Selective tropism of a neurotropic coronavirus for ependymal cells, neurons and meningeal cells. Journal of Virology 60:574–582
     [Google Scholar]
  32. Zurbringgen A., Fujinami R. S. 1988; Theilerʹs virus infection in nude mice: viral RNA in vascular endothelial cells. Journal of Virology 62:3569–3596
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-12-2867
Loading
Theiler’s Murine Encephalomyelitis Virus-binding Activity on Neural and Non-neural Cell Lines and Tissues
J. Gen. Virol. 71, 2867 (1990); https://doi.org/10.1099/0022-1317-71-12-2867
/content/journal/jgv/10.1099/0022-1317-71-12-2867
/content/journal/jgv/10.1099/0022-1317-71-12-2867
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error