Inhibition of Primary Transcription of Vesicular Stomatitis Virus by Prostaglandin A Free

Abstract

Prostaglandin A (PGA) exhibits antiviral activity against RNA and DNA viruses. The effect of PGA, on vesicular stomatitis virus (VSV) was investigated. When VSV-infected L-1210 cells were kept in the presence of PGA the amount of all five viral proteins and their respective mRNAs was dose-dependently decreased. To determine whether the effect was on viral transcription or translation, the temperature-sensitive VSV mutant tsG 41 was employed. This is a good model system for the investigation of primary transcription; at the restrictive temperature of 39 °C, tsG 41 is unable to replicate but can transcribe viral mRNA. Mutant mRNA synthesis was strongly inhibited by PGA, at this temperature, indicating that the major effect is on primary transcription. This conclusion is supported by data demonstrating that transcription of viral genomic RNA was also inhibited by PGA

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1990-12-01
2024-03-28
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References

  1. Ankel H., Mittnacht S., Jacobsen H. 1985; Antiviral activity of prostaglandin A on encaphalomyocarditis virus-infected cells: a unique effect unrelated to interferon. Journal of General Virology 66:2355–2364
    [Google Scholar]
  2. Banerjee A. K. 1987; The transcription complex of vesicular stomatitis virus. Cell 48:363–364
    [Google Scholar]
  3. Banerjee A. K., Moyer S. A., Rhodes D. P. 1974; Studies on the in vitro adenylation of RNA by vesicular stomatitis virus. Virology 61:547–558
    [Google Scholar]
  4. Benavente J., Esteban M., Jaffe B. M., Santoro M. G. 1984; Selective inhibition of viral gene expression as the mechanism of the antiviral action of PGA, in vaccinia virus-infected cells. Journal of General Virology 65:599–608
    [Google Scholar]
  5. Bito L. Z. 1972; Comparative study of concentrative prostaglandin accumulation by various tissues of mammals and marine vertebrates and invertebrates. Comparative Biochemistry and Physiology 43:65–82
    [Google Scholar]
  6. De B. P., Banerjee A. K. 1984; Specific interactions of vesicular stomatis virus L and NS proteins with heterologous genome ribonucleoprotein template lead to mRNA synthesis in vitro . Journal of Virology 51:628–634
    [Google Scholar]
  7. Emerson S. U., Yu Y. -H. 1975; Both NS and L protein are required for in vitro RNA synthesis by vesicular stomatitis virus. Journal of Virology 15:1348–1356
    [Google Scholar]
  8. Fukushima M., Kato T. 1984; Antineoplastic prostaglandin: antitumour effect of PGA and PGJ derivatives. In Icosanoids and Cancer pp. 275–278 Thaler-Dao H., De Paulet A. C., Paoletti R. Edited by New York: Raven Press;
    [Google Scholar]
  9. Fukushima M., Kato T., Ueda R., Ota K., Narumiya S., Hayaishi O. 1982; Prostaglandin D2, a potent antineoplastic agent. Biochemical and Biophysical Research Communications 105:956–964
    [Google Scholar]
  10. Gallione C. J., Greene J. R., Iverson L. E., Rose J. K. 1981; Nucleotide sequence of the mRNAs encoding the vesicular stomatitis virus N and NS proteins. Journal of Virology 39:529–535
    [Google Scholar]
  11. Goodwin J. S., Webb D. R. 1980; Regulation of the immune response by prostaglandins. Clinical Immunology and Immunopatho-logy 15:106–122
    [Google Scholar]
  12. Haas A. L., Bright P. M. 1985; The immunochemical detection and quantitation of intracellular ubiquitin-protein conjugates. Journal of Biological Chemistry 260:12464–12473
    [Google Scholar]
  13. Honda A., Mori Y., Iguchi K., Yamada Y. 1987; Antiproliferative and cytotoxic effects of newly discovered halogenated coral prostanoids from the Japanese stolonifer Clavularia viridis on human myeloid leukemia cells in culture. Molecular Pharmacology 32:530–535
    [Google Scholar]
  14. Honn K. V., Marnett L. J. 1985; Requirement of a reactive α, β- unsaturated carbonyl for inhibition of tumor growth and induction of differentiation by ‘A’ series prostaglandins. Biochemical and Biophysical Research Communications 129:34–40
    [Google Scholar]
  15. Langford M. P., Weigent D. A., Stanton G. J., Baron S. 1981; Virus plaque-reduction assay for interferon: microplaque and regular macroplaque reduction assays. Methods in Enzymology 78:339–351
    [Google Scholar]
  16. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Marcus P. I., Sekellick M. J. 1978; Interferon action III. The rate of primary transcription of vesicular stomatitis virus is inhibited by interferon action. Journal of General Virology 38:391–408
    [Google Scholar]
  18. Masters P. S., Samuel C. E. 1983b; Mechanism of interferon action: inhibition of vesicular stomatitis virus replication in human amnion U cells by cloned human leukocyte interferon. Journal of Biological Chemistry 258:12019–12033
    [Google Scholar]
  19. Narumiya S., Fukushima M. 1985; 12-prostagiandin J2, an ultimate metabolite of prostaglandin D2 exerting cell growth inhibition. Biochemical and Biophysical Research Communications 127:739–745
    [Google Scholar]
  20. Narumiya S., Fukushima M. 1986; Site and mechanism of growth inhibition by prostaglandins. I. Active transport and intracellular accumulation of cyclopentenone prostaglandins, a reaction leading to growth inhibition. Journal of Pharmacology and Experimental Therapeutics 239:500–505
    [Google Scholar]
  21. Nelson N. A., Kelly R. C., Johnson R. A. 1982; Prostaglandins and the arachidonic acid cascade. Chemical and Engineering News 60:30–45
    [Google Scholar]
  22. Ohno K., Fukushima M., Fujiwara M., Narumiya S. 1988a; Induction of 68,000-dalton heat shock proteins by cyclopentenone prostaglandins. Journal of Biological Chemistry 263:19764–19770
    [Google Scholar]
  23. Ohno K., Sakai T., Fukushima M., Narumiya S., Fujiwara M. 1988b; Site and mechanism of growth inhibition by prostaglandins. IV. Effect of cyclopentenone prostaglandins on cell cycle progression of G1-enriched HeLa S3 cells. Journal of Pharmacology and Experimental Therapeutics 254:294–298
    [Google Scholar]
  24. Pringle C. R. 1970; Genetic characteristics of conditional lethal mutants of vesicular stomatitis virus induced by 5-fluorouracil, 5-azacytidine, and ethyl methane sulfonate. Journal of Virology 5:559–567
    [Google Scholar]
  25. Rose J. K., Gallione C. J. 1981; Nucleotide sequence of the mRNAs encoding the vesicular stomatitis virus G and M proteins determined from cDNA clones containing the complete coding regions. Journal of Virology 39:519–528
    [Google Scholar]
  26. Rosen C. A., Ennis H. L., Cohen P. S. 1982; Translational control of vesicular stomatitis virus protein synthesis: isolation of an mRNA-sequestering particle. Journal of Virology 44:932–938
    [Google Scholar]
  27. Santoro M. G., Carruba G., Garaci E., Jaffe B. M. 1980; Prostaglandin A compounds as antiviral agents. Science 209:1032–1034
    [Google Scholar]
  28. Santoro M. G., Jaffe B., Elia G., Benedetto A. 1982; Prostaglandin A2 induces the synthesis of a new protein in cultured AGMK cells. Biochemical and Biophysical Research Communications 4:1179–1184
    [Google Scholar]
  29. Santoro M. G., Jaffe B. M., Esteban M. 1983a; Prostaglandin A inhibits the replication of vesicular stomatitis virus: effect on virus glycoprotein. Journal of Genera! Virology 64:2797–2801
    [Google Scholar]
  30. Santoro M. G., Jaffe B. M., Paez E., Esteban M. 1983b; The relationship between the antiviral action of interferon and prostaglandins in virus-infected murine cells. Biochemical and Biophysical Research Communications 116:442–448
    [Google Scholar]
  31. Santoro M. G., Crisari A., Benedetto A., Amici C. 1986; Modulation of the growth of a human erythroleukemic cell line (K562) by prostaglandins: antiproliferative action of prostaglandin A1 . Cancer Research 46:6073–6077
    [Google Scholar]
  32. Santoro M. G., Fukushima M., Benedetto A., Amici C. 1987; PGJ2, a new antiviral prostaglandin: inhibition of Sendai virus replication and alteration of virus protein synthesis. Journal of General Virology 68:1153–1158
    [Google Scholar]
  33. Santoro M. G., Favalli C., Mastino A., Jaffe B. M., Esteban M., Garaci E. 1988; Antiviral activity of a synthetic analog of prostaglandin A in mice infected with influenza A virus. Archives of Virology 99:89–100
    [Google Scholar]
  34. Santoro M. G., Amici C., Elia G., Benedetto A., Garaci E. 1989a; Inhibition of virus protein glycosylation as the mechanism of the antiviral action of prostaglandin A in Sendai virus-infected cells. Journal of General Virology 70:789–800
    [Google Scholar]
  35. Santoro M. G., Garaci E., Amici C. 1989b; Prostaglandins with antiproliferative activity induce the synthesis of a heat shock protein in human cells. Proceedings of the National Academy of Sciences, U.S.A 86:8407–8411
    [Google Scholar]
  36. Schubert M., Harmison G. G., Meier E. 1984; Primary' structure of the vesicular stomatitis virus polymerase (L) gene: evidence for a high frequency of mutations. Journal of Virology 51:505–514
    [Google Scholar]
  37. Whittle B. J., Moncada S., Vane J. R. 1978; Comparison of the effects of prostacyclin (PGJ2), prostaglandin E1 and D2 on platelet aggregation in different species. Prostaglandins 16:373–380
    [Google Scholar]
  38. Yamamoto N., Fukushima M., Tsurumi T., Maeno K., Nishiyama Y. 1987; Mechanism of inhibition of herpes simplex virus replication by ∆7-prostaglandin A1 and ∆12--prostaglandin J,. Biochemical and Biophysical Research Communications 146:1425–1431
    [Google Scholar]
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