The 5′ and 3′ termini of four ssRNA species of rice stripe virus (RSV) isolate T were sequenced. The 3′ termini of the three smallest ssRNAs, i.e. RNAs 2, 3 and 4, had the sequence 5′ GACUUUGUGU 3′; that of ssRNA 1 had the sequence 5′ GACUAUGUGU 3′. The 5′-terminal sequences of all four ssRNAs were 5′ ACACAAAGUCC 3′. The 5′- and 3′-terminal sequences of about 20 bases of each ssRNA were almost complementary to each other. It is possible that RSV RNAs form panhandle structures characteristic of the RNA of negative-strand viruses.


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