1887

Abstract

The complete nucleotide sequence of barley yellow mosaic virus (BaYMV) RNA 1 was obtained by analysis of overlapping cDNA clones and by direct RNA sequencing. The sequence is 7632 nucleotides in length, excluding a 3′ poly(A) tail. The first AUG codon at nucleotide 172 appeared to be the initiator for a single long open reading frame encoding a protein of 2410 amino acids with an of 270755. Amino acid sequence comparisons revealed that the BaYMV 270K protein contains three regions upstream of the C- terminal capsid protein which share significant homologies with the cytoplasmic inclusion and two nuclear inclusion proteins of potyviruses thus indicating their similarities in genetic organization. However, the apparent low levels of homology in the corresponding proteins of BaYMV and potyviruses are in contrast with the high conservation among potyviruses. Moreover, our data indicate that BaYMV RNA 1 has no counterpart to the two cistrons located in the 5′- terminal region of the potyvirus genome. Although the data suggest a close evolutionary relationship between BaYMV and potyviruses, the striking differences set BaYMV apart from potyviruses.

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1990-12-01
2022-10-07
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References

  1. Allison R., Johnston R. E., Dougherty W. G. 1986; The nucleotide sequence of the coding region of tobacco etch virus genomic RNA; evidence for the synthesis of a single polyprotein. Virology 154:9–20
    [Google Scholar]
  2. Carrington J. C., Dougherty W. G. 1988; A viral cleavage site cassette: identification of amino acid sequences required for tobacco etch virus polyprotein processing. Proceedings of the National Academy of Sciences U.S.A: 853391–3395
    [Google Scholar]
  3. Carrington J. C., Cary S. M., Dougherty W. G. 1988; Mutational analysis of tobacco etch virus polyprotein processing: cis and trans proteolytic activities of polyproteins containing 46-kilodalton proteinase. Journal of Virology 62:2313–2320
    [Google Scholar]
  4. Carroll A. R., Rowlands D. J., Clarke B. E. 1984; The complete nucleotide sequence of the RNA coding for the primary translation product of foot and mouth disease virus. Nucleic Acids Research 12:2461–2472
    [Google Scholar]
  5. Domier L. L., Franklin K. M., Shahabuddin M., Hellmann G. M., Overmeyer J. M., Hiremath S. T., Siaw M.F.E., Lomonossoff G. P., Shaw J. G., Rhoads R. E. 1986; The nucleotide sequence of tobacco vein mottling virus RNA. Nucleic Acids Research 14:5417–5430
    [Google Scholar]
  6. Domier L.L, Shaw J. G., Rhoads R. E. 1987; Potyviral proteins share amino acid sequence homology with picorna-, como-, and caulimoviral proteins. Virology 158:20–27
    [Google Scholar]
  7. Dougherty W. G., Carrington J. C. 1988; Expression and function of potyviral gene products. Annual Review of Phytopathology 26:123–143
    [Google Scholar]
  8. Dougherty W. G., Parks T. D. 1989; Molecular genetic and biochemical evidence for the involvement of the heptapeptide cleavage sequence in determining the reaction profile at two tobacco etch cleavage sites in cell-free assays. Virology 172:145–155
    [Google Scholar]
  9. Dougherty W. G., Cary S. M., Parks T. D. 1989a; Molecular genetic analysis of a plant virus polyprotein cleavage site: a model. Virology 171:356–364
    [Google Scholar]
  10. Dougherty W. G., Parks T. D., Cary S. M., Bazan J. F., Fletterick R. J. 1989b; Characterization of the catalytic residues of tobacco etch virus 49-KDa proteinase. Virology 172:302–310
    [Google Scholar]
  11. Frenkel M. J., Ward C. W., Shukla D. D. 1989; The use of 3′ non-coding sequences in the taxonomy of potyviruses: application to watermelon mosaic virus 2 and soybean mosaic virus-N. Journal of General Virology 70:2775–2783
    [Google Scholar]
  12. Gallie D. R., Sleat D. E., Watts J. W., Turner P. C., Wilson T.M. 1987; A comparison of eukaryotic viral 5′-leader sequences as enhancers of mRNA expression in vitro . Nucleic Acids Research 15:8693–8711
    [Google Scholar]
  13. García J. A., Riechmann J. L., Laín S. 1989; Proteolytic activity of the plum pox potyvirus NIa-like protein in Escherichia coli . Virology 170:362–369
    [Google Scholar]
  14. Geliebter J. 1987; Dideoxynucleotide sequencing of RNA and uncloned cDNA. Focus 95–8 Gaithersburg: Bethesda Research Laboratories;
    [Google Scholar]
  15. Goldbach R. W. 1986; Molecular evolution of plant RNA viruses. Annual Review of Phytopathology 24:289–310
    [Google Scholar]
  16. Goldbach R. W. 1985; Structure, replication and expression of the bipartite genome of cowpea mosaic virus. In Molecular Plant Virology 2 pp 83–120 Davies J. W. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  17. Goldbach R., Wellink J. 1988; Evolution of plus-strand RNA viruses. Intervirology 29:260–267
    [Google Scholar]
  18. Gorbalenya A. E., Koonin E. V. 1989; Viral proteins containing the purine NTP-binding sequence pattern. Nucleic Acids Research 17:8413–8440
    [Google Scholar]
  19. Gorbalenya A. E., Koonin E. V., Donchenko A. P., Blinov V. M. 1988; A novel superfamily of nucleoside triphosphatebinding motif containing proteins which are probably involved in duplex unwinding in DNA and RNA replication and recombination. FEBS Letters 235:16–24
    [Google Scholar]
  20. Gorbalenya A. E., Donchenko A. P., Blinov V. M., Koonin E. V. 1989a; Cysteine proteases of positive strand RNA viruses and chymotrypsin-like serine proteases. A distinct protein superfamily with a common structural fold. FEBS Letters 243:103–114
    [Google Scholar]
  21. Gorbalenya A. E., Koonin E. V., Donchenko A. P., Blinov V. M. 1989b; Coronavirus genome: prediction of putative functional domains in the non-structural polyprotein by comparative amino acid sequence analysis. Nucleic Acids Research 17:4847–4861
    [Google Scholar]
  22. Greif C., Hemmer O., Fritsch C. 1988; Nucleotide sequence of tomato black ring virus RN A-1. Journal of General Virology 69:1517–1529
    [Google Scholar]
  23. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  24. Hibino H., Usugi T., Saito Y. 1981; Comparative electron microscopy of inclusions associated with five soil-borne filamentous viruses of cereals. Annals of the Phytopathological Society of Japan 47:510–519
    [Google Scholar]
  25. Inouye T., Saito Y. 1975; Barley yellow mosaic virus. CMI/AAB Descriptions of Plant Viruses143
    [Google Scholar]
  26. Kamer G., Argos P. 1984; Primary structural comparison of RNA-dependent RNA polymerases from plant, animal and bacterial viruses. Nucleic Acids Research 12:7269–7282
    [Google Scholar]
  27. Kashiwazaki S., Hayano Y., Minobe Y., Omura T., Hibino H., Tsuchizaki T. 1989a; Nucleotide sequence of the capsid protein gene of barley yellow mosaic virus. Journal of General Virology 70:3015–3023
    [Google Scholar]
  28. Kashiwazaki S., Ogawa K., Usugi T., Omura T., Tsuchizaki T. 1989b; Characterization of several strains of barley yellow mosaic virus. Annals of the Phytopathological Society of Japan 55:16–25
    [Google Scholar]
  29. Kelly R. C., Jensen D. E., Von Hippel P. H. 1976; DNA ‘melting’ proteins. IV. Fluorescence measurements of binding parameters for bacteriophage T4 gene 32-protein to mono-, oligo-, and polynucleotides. Journal of Biological Chemistry 251:7240–7250
    [Google Scholar]
  30. Kitamura N., Semler B. L., Rothberg P. G., Larsen G. R., Adler C. J., Dorner A. J., Emini E. A., Hanecak R., Lee J. J., Vanderwerf S., Anderson C. W., Wimmer E. 1981; Primary structure, gene organization and polypeptide expression of poliovirus RNA. Nature; London: 291547–553
    [Google Scholar]
  31. Kozak M. 1984; Compilation and analysis of sequences upstream from translational start in eukaryotic mRNA. Nucleic Acids Research 12:857–872
    [Google Scholar]
  32. Kräusslich H. -G., Wimmer E. 1988; Viral proteinases. Annual Review of Biochemistry 57:701–754
    [Google Scholar]
  33. Laín S., Riechmann J. L., García J. A. 1989; The complete nucleotide sequence of plum pox potyvirus RNA. Virus Research 13:157–172
    [Google Scholar]
  34. Lomonossoff G., Shanks M. 1983; The nucleotide sequence of cowpea mosaic virus B RNA. EMBO Journal 2:2253–2258
    [Google Scholar]
  35. Lütcke H. A., Chow K. C., Mickel F. S., Moss K. A., Kern H. F., Scheele G. A. 1987; Selection of AUG initiation codons differs in plants and animals. EMBO Journal 6:43–48
    [Google Scholar]
  36. Maiss E., Timpe U., Brisske A., Jelkmann W., Casper R., Himmler G., Mattanovich D., Katinger H.W.D. 1989; The complete nucleotide sequence of plum pox virus RNA. Journal of General Virology, 70:513–524
    [Google Scholar]
  37. Palmenberg A. C., Kirby E. M., Janda M. R., Drake N. L., Duke G. M., Potratz K. F., Collett M. S. 1984; The nucleotide and deduced amino acid sequences of the encephalomyo- carditis viral polyprotein coding region. Nucleic Acids Research 12:2969–2985
    [Google Scholar]
  38. Quemada H., L’Hostis B., Gonsalves D., Reardon I. M., Heinrikson R., Hiebert E. L., Sieu L. C., Slightom J. L. 1990; The nucleotide sequences of the 3′-terminal regions of papaya ringspot virus strains W and P. Journal of General Virology 71:2032–10
    [Google Scholar]
  39. Riechmann J. L., Laín S., García J. A. 1989; The genome-linked protein and 5′ end RNA sequence of plum pox potyvirus. Journal of General Virology 70:2785–2789
    [Google Scholar]
  40. Robaglia C., Durand-Tardif M., Tronchet M., Boudazin G., Astier-Manifacier S., Casse-Delbart F. 1989; Nucleotide sequence of potato virus Y (N strain) genomic RNA. Journal of General Virology 70:935–947
    [Google Scholar]
  41. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erich H. A. 1988; Primer-directed enzyme amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
    [Google Scholar]
  42. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences U.S.A: 745463–5467
    [Google Scholar]
  43. Takeda N., Kuhn R. J., Yang C., Takegami T., Wimmer E. 1986; Initiation of poliovirus plus-strand RNA synthesis in a membrane complex of infected HeLa cells. Journal of Virology 60:43–53
    [Google Scholar]
  44. Takegami T., Semler B. L., Anderson C. W., Wimmer E. 1983; Membrane fractions active in poliovirus RNA replication contain VPg precursor polypeptides. Virology 128:33–47
    [Google Scholar]
  45. Turpen T. 1989; Molecular cloning of a potato virus Y genome: nucleotide sequence homology in non-coding regions of potyviruses. Journal of General Virology 70:1951–1960
    [Google Scholar]
  46. Usugi T., Saito Y. 1976; Purification and serological properties of barley yellow mosaic virus and wheat yellow mosaic virus. Annals of the Phytopathological Society of Japan 42:12–20
    [Google Scholar]
  47. Usugi T., Kashiwazaki S., Omura T., Tsuchizaki T. 1989; Some properties of nucleic acids and coat proteins of soil-borne filamentous viruses. Annals of the Phytopathological Society of Japan 55:26–31
    [Google Scholar]
  48. Wellink J., Van Kammen A. 1988; Proteases involved in the processing of viral polyproteins. Archives of Virology 98:1–26
    [Google Scholar]
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