1887

Abstract

Synthetic peptides, recombinant fusion proteins and mouse monoclonal antibodies were used to delineate a B cell epitope of the VP′ structural protein of canine parvovirus (CPV). Although this epitope is not preferentially recognized in the normal antibody response to CPV, virus-specific antibodies could be induced in BALB/c mice with a synthetic peptide representing the epitope. The potential of this non-dominant B cell epitope to induce antiviral immunity in the presence of maternal CPV-specific antibodies, is discussed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-11-2741
1990-11-01
2021-10-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/11/JV0710112741.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-11-2741&mimeType=html&fmt=ahah

References

  1. Carlson J., Rushlov K., Maxwell I., Maxwell F., Winston S., Hahn W. 1985; Cloning and sequence of DNA-encoding structural proteins of the autonomous parvovirus feline panleukopenia virus. Journal of Virology 55:574–582
    [Google Scholar]
  2. Geysen H. M., Meloen R. H., Barteling S. 1984; Use of peptide synthesis to probe viral antigens for epitopes to a resolution of a single amino acid. Proceedings of the National Academy of Sciences, U.S.A 81:3998
    [Google Scholar]
  3. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  4. Paradiso P. R., Rhode S. L. III Singer I. I. 1982; Canine parvovirus: a biochemical and ultrastructural characterization. Journal of General Virology 62:113–125
    [Google Scholar]
  5. Parrish C. R., Carmichael L. E. 1983; Antigenic structure and variation of canine parvovirus type-2, feline panleukopenia virus and mink enteritis virus. Virology 129:401–414
    [Google Scholar]
  6. Parrish C. R., Carmichael L. E., Antczak D. F. 1982; Antigenic relationships between canine parvovirus type 2, feline panleukopenia virus and mink enteritis virus using conventional antisera and monoclonal antibodies. Archives of Virology 72:267–278
    [Google Scholar]
  7. Parrish C. R., Aquadro C. F., Carmichael L. E. 1988; Canine host range and a specific epitope map along with variant sequences in the capsid protein gene of canine parvovirus and related feline, mink and raccoon parvoviruses. Virology 166:293–307
    [Google Scholar]
  8. Pollock R. V. H., Carmichael L. E. 1982; Maternally derived immunity to canine parvovirus infection: transfer, decline and interference with vaccination. Journal of the American Veterinary Medical Association 180:37–43
    [Google Scholar]
  9. Reed A. P., Jones E. V., Miller T. J. 1988; Nucleotide sequence and genome organization of canine parvovirus. Journal of Virology 62:266–276
    [Google Scholar]
  10. Rhode S. L. 1985; Nucleotide sequence of the coat protein gene of canine parvovirus. Journal of Virology 54:630–633
    [Google Scholar]
  11. Rimmelzwaan G. F., Groen J., Juntti N., Teppema J. S., Uytdehaag F. G. C. M., Osterhaus A. D. M. E. 1987; Purification of infectious canine parvovirus from cell culture by affinity chromatography with monoclonal antibodies. Journal of Virological Methods 15:313–322
    [Google Scholar]
  12. Rimmelzwaan G. F., Juntti N., Klingeborn B., Groen J., Uytdehaag F. G. C. M., Osterhaus A. D. M. E. 1990a; Evaluation of enzyme-linked immunosorbent assays based on monoclonal antibodies for the serology and antigen detection in canine parvovirus infections. Veterinary Quarterly 12:14–20
    [Google Scholar]
  13. Rimmelzwaan G. F., van der Heijden R. W. J., Tijhaar E., Poelen M. C. M., Carlson J., Osterhaus A. D. M. E., Uytdehaag F. G. C. M. 1990b; Establishment and characterization of canine parvovirus-specific murine CD4+ T cell clones and their use for the delineation of T cell epitopes. Journal of General Virology 71:1095–1102
    [Google Scholar]
  14. Rimmelzwaan G. F., Poelen M. C. M., Meloen R. H., Carlson J., Uytdehaag F. G. C. M., Osterhaus A. D. M. E. 1990c; Delineation of canine parvovirus T cell epitopes with peripheral blood mononuclear cells and T cell clones from immunized dogs. Journal of General Virology 71:2321–2329
    [Google Scholar]
  15. Surleraux M., Bodeus M., Burtonboy G. 1987; Study of canine parvovirus polypeptides by immunoblot analysis. Archives of Virology 95:271–281
    [Google Scholar]
  16. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A 76:4350–4354
    [Google Scholar]
  17. Uytdehaag F. G. C. M., Osterhaus A. D. M. E., Loggen H. G., Barker R. H. J., van Asten J.A.A.M., Kreeftenberg J. G., van Der Marel P., van Steenis B. 1983; Induction of an antigenic-specific antibody response in human peripheral blood lymphocytes in vitro by a dog kidney cell vaccine against rabies virus (DKCV). Journal of Immunology 131:1234–1239
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-11-2741
Loading
/content/journal/jgv/10.1099/0022-1317-71-11-2741
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error