1887

Abstract

The topographical distribution of the poliovirus receptor on the cell surface was demonstrated by immunoelectron microscopy using monoclonal antibodies and immunogold markers. The receptor appeared in small clusters, which were randomly distributed over the cell surface and along cellular processes. The distribution pattern of the clusters corresponded to that of adsorbed and immunogold- labelled poliovirus particles and suggests a multivalent organization of poliovirus binding sites. Freezefracturing and ultrathin sectioning did not reveal any specific ultrastructures within the plasma membrane at labelled receptor areas. Incubation of native cells with anti-receptor antibodies did not remove the receptor molecule from the cell surface nor did it induce ultrastructural alterations within the plasma membrane. The antibody-receptor complexes exhibited lateral mobility within the plasma membrane and were able to aggregate into large immune complexes after incubation with a second ligand.

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1990-11-01
2021-10-26
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References

  1. Colonno R. J. 1986; Cell surface receptors for picornaviruses. BioEssays 5:270–274
    [Google Scholar]
  2. Crowell R. L., Landau B. J. 1983; Receptors in the initiation of picornavirus infections. In Comprehensive Virology 18 pp. 1–42 Fraenkel-Conrat H., Wagner R. R. Edited by New York: Plenum Press;
    [Google Scholar]
  3. Dunnebacke T. H., Levinthal J. D., Williams R. C. 1969; Entry and release of poliovirus as observed by electron microscopy of cultured cells. Journal of Virology 4:505–513
    [Google Scholar]
  4. Dimmock N. J. 1982; Initial stages in infection with animal viruses. Journal of General Virology 59:1–22
    [Google Scholar]
  5. Guttman N., Baltimore D. 1977; A plasma membrane component able to bind and alter virions of poliovirus type 1: studies on cell-free alteration using a simplified assay. Virology 82:25–36
    [Google Scholar]
  6. Hohenberg H. 1989; Replica preparation techniques in immunogold cytochemistry. In Immuno-gold Labeling in Cell Biology pp. 157–177 Verkleij A. J., Leunissen J. L. M. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  7. Hohenberg H., Bohn W., Rutter G., Mannweiler K. 1985; Plasma membrane antigens detected by replica techniques. In Science of Biological Specimen Preparation pp. 235–244 O’Hare A. M. F. Edited by Chicago: SEM;
    [Google Scholar]
  8. Holland J. J., Hoyer B. 1962; Early stages in enterovirus infection. Cold Spring Harbor Symposia on Quantitative Biology 27:101–112
    [Google Scholar]
  9. Mannweiler K., Hohenberg H., Bohn W., Rutter G. 1982; Protein-A gold particles as markers in replica immuno-cytochemistry: high resolution electron microscope investigations of plasma membrane surfaces. Journal of Microscopy 126:145–149
    [Google Scholar]
  10. Mendelsohn C. L., Wimmer E., Racaniello V. R. 1989; Cellular receptor for poliovirus: molecular cloning, nucleotide sequence, and expression of a new member of the immunoglobulin superfamily. Cell 56:855–865
    [Google Scholar]
  11. Miller D. A., Miller O. J., Dev V. G., Hashmi S., Tantravahi R., Medrano L., Green H. 1974; Human chromosome 19 carries a poliovirus receptor gene. Cell 1:167–170
    [Google Scholar]
  12. Minor P. D., Pepkin P. A., Hockley D., Schild G. C., Almond J. W. 1984; Monoclonal antibodies which block cellular receptors of poliovirus. Virus Research 1:203–212
    [Google Scholar]
  13. Nobis P., Zibirre R., Meyer G., Kühne J., Warnecke G., Koch G. 1985; Production of monoclonal antibody against an epitope on HeLa cells that is the functional poliovirus binding site. Journal of General Virology 66:2563–2569
    [Google Scholar]
  14. Pintodasilva P., Khan F. W. K. 1984; Label-fracture. Journal of Cell Biology 99:1156–1161
    [Google Scholar]
  15. Shepley M. P., Sherry B., Weiner H. L. 1988; Monoclonal antibody identification of a 100-kDa membrane protein in HeLa cells and human spinal cord involved in poliovirus attachment. Proceedings of the National Academy of Sciences, U.S.A 85:7743–7747
    [Google Scholar]
  16. Zeichhardt H., Wetz K., Willingmann P., Habermehl K.-O. 1985; Entry of poliovirus type 1 and Mouse Elberfeld (ME) virus into HEp-2 cells: receptor-mediated endocytosis and endosomal or lysosomaluncoating. Journal of General Virology 66:483–492
    [Google Scholar]
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