@article{mbs:/content/journal/jgv/10.1099/0022-1317-71-11-2673, author = "Kari, Bruce and Liu, Yung-Nan C. and Goertz, Ron and Lussenhop, Nancy and Stinski, Mark F. and Gehrz, Richard", title = "Structure and composition of a family of human cytomegalovirus glycoprotein complexes designated gC-I (gB)", journal= "Journal of General Virology", year = "1990", volume = "71", number = "11", pages = "2673-2680", doi = "https://doi.org/10.1099/0022-1317-71-11-2673", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-71-11-2673", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Murine rhonoclonal antibodies (MAbs) were made to the 52000 (gp52) and the 93000 to 130000 Mr (gp93–130) glycoproteins from a human cytomegalovirus (HCMV) glycoprotein complex designated gC-I or the gB homologue. MAbs recognizing either gp52 or gp93–130 could immunoprecipitate unreduced gC-I complexes from non-ionic detergent extracts of HCMV. Western blotting was performed with immunoaffmity- purified gC-I complexes which were reduced prior to analysis. MAbs made against gp52 recognized gp52 and a 158000 Mr glycoprotein (gpl58). MAbs which recognized gp93–130 in a Western blot also reacted with gpl58, which is a gC-I precursor glycoprotein. The origin of gp93–130 was demonstrated by the reactivity of our gp93–130 MAbs with a recombinant protein containing the N-terminal portion of the gB gene. These data are consistent with the hypothesis that gp52 and gp93–130 are generated from the same high Mr precursor by proteolysis. MAbs recognizing either gp52 or gp93–130 neutralized Towne strain HCMV, but MAbs recognizing gp52 required complement to neutralize whereas MAbs recognizing gp93–130 did not. It was also determined that gp93–130 and gpl58 have detectable amounts of 0-linked glycans but gp52 does not, showing a difference in the glycosyla- tion of these glycoproteins. Analysis of gC-I disulphide bonds showed that two types were present, one which was very susceptible to reduction and a second which was less susceptible. These complexes could consist of very susceptible inter-complex disulphide bonds and less susceptible intra-complex disulphide bonds.", }