More than 100 isolates were plaque-purified to examine the genetic variations in four wild stocks of . Nuclear polyhedrosis virus (NPV) collected in Japan. These isolates were characterized by their host range in three established insect cell lines, growth characteristics, polyhedral protein, DNA restriction endonuclease pattern and DNA hybridization. The isolates were separated into four distinct groups: (I) isolates corresponding to NPV, (II and IV) two different groups of isolates of NPV which had been previously characterized and (III) isolates with no correspondence to any reported virus group. Of the NPV wild stocks, two were mixtures of more than two different groups of NPVs. We have discussed the advantage of having a mixture of different NPV groups in the same wild virus stocks.


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