1887

Abstract

Cross-reactive neutralization epitopes on VP4 of human rotavirus (HRV) were analysed by the use of VP4-specific neutralizing monoclonal antibodies (N-MAbs) and MAb-resistant mutants. Seven anti-VP4 N-MAbs obtained in this study by using HRV serotypes 1 and 3 as immunizing antigens showed a variety of cross-reactivity patterns to 20 HRV strains with different serotype specificity in neutralization tests and a broader cross-reactivity to them was found for four N-MAbs in an enzyme-linked immunosorbent assay. On the basis of the reactivity patterns against rotaviruses in neutralization tests, these seven N-MAbs were classified into four groups. Cross-neutralization tests using a total of 12 pairs of MAbs and resistant mutants, including five pairs which had been prepared previously, showed that VP4 of HRV (strain KU) contained two independent antigenic regions. One, region C1, was recognized by a single MAb (YO-2C2) and the other was made up of two antigenic regions (C2 and C3) which overlapped operationally. Identification of amino acid substitution sites on VP4 of representative mutants of HRV strain KU indicated that amino acid positions 385 or 392 and 428 or 433 were critical for the C2 and C3 regions, respectively. These results suggested that regions C2 and C3 exist as conformational antigenic sites.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-11-2615
1990-11-01
2024-07-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/11/JV0710112615.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-11-2615&mimeType=html&fmt=ahah

References

  1. Burns J. W., Greenberg H. B., Shaw R. D., Estes M. K. 1988; Functional and topographical analyses of epitopes on the haemagg-lutinin (VP4) of the simian rotavirus SA11. Journal of Virology 62:2164–2172
    [Google Scholar]
  2. Coulson B. S., Tursi J. M., Mcadam W. J., Bishop R. F. 1986; Derivation of neutralizing monoclonal antibodies to human rotaviruses and evidence that an immunodominant neutralization site is shared between serotypes 1 and 3. Virology 154:301–312
    [Google Scholar]
  3. Dyall-Smith M. L., Lazdins I., Tregear G. W., Holmes I. H. 1986; Location of the major antigenic sites involved in rotavirus serotype-specific neutralization. Proceedings of the National Academy of Sciences, U.S.A 83:3465–3468
    [Google Scholar]
  4. Espejo R. T., Lopez S., Arias C. F. 1981; Structural polypeptides of simian rotavirus SA11 and the effect of trypsin. Journal of Virology 37:156–160
    [Google Scholar]
  5. Estes M. K., Graham D. Y., Mason B. B. 1981; Proteolytic enhancement of rotavirus infectivity: molecular mechanisms. Journal of Virology 39:879–888
    [Google Scholar]
  6. Estes M. K., Palmer E. L., Obijeski J. F. 1983; Rotaviruses: a review. Current Topics in Microbiology and Immunology 105:123–184
    [Google Scholar]
  7. Flores J., Greenberg H. B., Myslinski J., Kalica A. R., Wyatt R. G., Kapikian A. Z., Chanock R. M. 1982; Use of transcription probes for genotyping rotavirus reassortants. Virology 121:288–295
    [Google Scholar]
  8. Flores J., Midthun K., Hoshino Y., Green K., Gorziglia M., Kapikian A. Z., Chanock R. M. 1986; Conservation of the fourth gene among rotaviruses recovered from asymptomatic newborn infants and its possible role in attenuation. Journal of Virology 60:972–979
    [Google Scholar]
  9. Gorziglia M., Hoshino Y., Buckler-White A., Blumentals I., Glass R., Flores J., Kapikian A. Z., Chanock R. M. 1986; Conservation of amino acid sequence of VP8 and cleavage region of 84-kDa outer capsid protein among rotaviruses recovered from asymptomatic neonatal infection. Proceedings of the National Academy of Sciences, U.S.A 83:7039–7043
    [Google Scholar]
  10. Gorziglia M., Green K. Y., Nishikawa K., Taniguchi K., Jones R., Kapikian A. Z., Chanock R. M. 1988; Sequence of the fourth gene of human rotaviruses recovered from asymptomatic or symptomatic infections. Journal of Virology 62:2978–2984
    [Google Scholar]
  11. Greenberg H. B., Kalica A. R., Wyatt R. G., Jones R. W., Kapikian A. Z., Chanock R. M. 1981; Rescue of noncultiva-table human rotavirus by gene reassortment during mixed infection with ts mutants of cultivatable bovine rotavirus. Proceedings of the National Academy of Sciences, U.S.A 78:420–424
    [Google Scholar]
  12. Greenberg H. B., Flores J., Kalica A. R., Wyatt R. G., Jones R. 1983; Gene coding assignments for growth restriction, neutralization and subgroup specificities of the W and DS-1 strains of human rotavirus. Journal of General Virology 64:313–320
    [Google Scholar]
  13. Hoshino Y., Wyatt R. G., Flores J., Midthun K., Kapikian A. Z. 1985; Serotypic characterization of rotaviruses derived from asymptomatic human neonatal infection. Journal of Clinical Microbiology 21:425–430
    [Google Scholar]
  14. Kalica A. R., Greenberg H. B., Wyatt R. G., Flores J., Senero M. M., Kapikian A. Z., Chanock R. M. 1981; Genes of human (strain Wa) and bovine (strain UK) rotaviruses that code for neutralization and subgroup antigens. Virology 112:385–390
    [Google Scholar]
  15. Kalica A. R., Flores J., Greenberg H. B. 1983; Identification of the rotaviral gene that codes for the hemagglutinin and protease-enhanced plaque formation. Virology 125:194–205
    [Google Scholar]
  16. Kaljot K. T., Shaw R. D., Rubin D. H., Greenberg H. B. 1988; Infectious rotavirus enters cells by direct cell membrane penetration, not by endocytosis. Journal of Virology 62:1136–1144
    [Google Scholar]
  17. Kapikian A. Z., Chanock R. M. 1985; Rotaviruses. In Virology pp. 863–906 Fields B. N., Knipe D. M., Chanock R. M., Melnick J. L., Roizman B., Shope R. E. Edited by New York: Raven Press;
    [Google Scholar]
  18. Kapikian A. Z., Flores J., Hoshino Y., Glass R.L, Gorziglia M., Chanock R. M. 1986; Rotavirus: the major etiologic agent of severe infantile diarrhea may be controllable by a ‘Jennerian’ approach to vaccination. Journal of Infectious Diseases 153:815–822
    [Google Scholar]
  19. Kitaoka S., Fukuhara N., Tazawa F., Suzuki H., Sato T., Konno T., Ebina T., Ishida N. 1986; Characterization of monoclonal antibodies against human rotavirus hemagglutinin. Journal of Medical Virology 19:313–323
    [Google Scholar]
  20. Kjellén L. 1962; Studies on the interactions of adenovirus, antibody, and host cells in vitro. Virology 18:448–456
    [Google Scholar]
  21. Mackow E. R., Shaw R. D., Matsui S. M., Vo P. T., Benfield D. A., Greenberg H. B. 1988a; Characterization of homotypic and heterotypic VP7 neutralization sites of rhesus rotavirus. Virology 165:511–517
    [Google Scholar]
  22. Mackow E. R., Shaw R. D., Matsui S. M., Vo P. T., Dang M.-N., Greenberg H. B. 1988b; The rhesus rotavirus gene encoding protein VP3: location of amino acids involved in homologous and heterologous rotavirus neutralization and identification of a putative fusion region. Proceedings of the National Academy ofSciences, U.S.A 85:645–649
    [Google Scholar]
  23. Mason B. B., Graham D. Y., Estes M. K. 1983; Biochemical mapping of the simian rotavirus SA11 genome. Journal of Virology 46:413–423
    [Google Scholar]
  24. Morita Y., Taniguchi K., Urasawa T., Urasawa S. 1988; Analysis of serotype-specific neutralization epitopes on VP7 of human rotavirus by the use of neutralizing monoclonal antibodies and antigenic variants. Journal of General Virology 69:451–458
    [Google Scholar]
  25. Nishikawa K., Taniguchi K., Torres A., Hoshino Y., Green K. Y., Kapikian A. Z., Chanock R. M., Gorziglia M. 1988; Comparative analysis of the VP3 gene of divergent strains of the rotaviruses simian SA11 and bovine Nebraska calf diarrhea virus. Journal of Virology 62:4022–4026
    [Google Scholar]
  26. Offit P. A., Blavat G. 1986; Identification of the two rotavirus genes determining neutralization specificities. Journal of Virology 57:376–378
    [Google Scholar]
  27. Offit P. A., Clark H. F., Stroop W. G., Twist E. M., Plotkin S. A. 1983; The cultivation of human rotavirus, strain ‘Wa,’ to high titer in cell culture and characterization of the viral structural polypeptides. Journal of Virological Methods 7:29–40
    [Google Scholar]
  28. Offit P. A., Shaw R. D., Greenberg H. B. 1986; Passive protection against rotavirus-induced diarrhea by monoclonal antibodies to surface proteins vp3 and vp7. Journal of Virology 58:700–703
    [Google Scholar]
  29. Shaw R. D., Vo P. T., Offit P. A., Coulson B. S., Greenberg H. B. 1986; Antigenic mapping of the surface proteins of rhesus rotavirus. Virology 155:434–451
    [Google Scholar]
  30. Stocker J. W., Forster H. K., Miggiano V., Stähli C., Staiger G., Takacs B., Staehelin T. 1982; Generation of 2 new mouse myeloma cell lines ‘PAI’ and ‘PAI-O’ for hybridoma production. Research Disclosure 217:155–157
    [Google Scholar]
  31. Taniguchi K., Urasawa S., Urasawa T. 1983; Monoclonal antibodies reactive with different sites of N and H antigenic particles of poliovirus. Journal of General Virology 64:2803–2808
    [Google Scholar]
  32. Taniguchi K., Urasawa S., Urasawa T. 1985; Preparation and characterization of neutralizing monoclonal antibodies with different reactivity patterns to human rotaviruses. Journal of General Virology 66:1045–1053
    [Google Scholar]
  33. Taniguchi K., Morita Y., Urasawa T., Urasawa S. 1987; Cross-reactive neutralization epitopes on VP3 of human rotavirus: analysis with monoclonal antibodies and antigenic variants. Journal of Virology 61:1726–1730
    [Google Scholar]
  34. Taniguchi K., Hoshino Y., Nishikawa K., Green K. Y., Maloy W. L., Morita Y., Urasawa S., Kapikian A. Z., Chanock R. M., Gorziglia M. 1988a; Cross-reactive and serotype-specific neutralization epitopes on VP7 of human rotavirus: nucleotide sequence analysis of antigenic mutants selected with monoclonal antibodies. Journal of Virology 62:1870–1874
    [Google Scholar]
  35. Taniguchi K., Maloy W. L., Nishikawa K., Green K. Y., Hoshino Y., Urasawa S., Kapikian A. Z., Chanock R. M., Gorziglia M. 1988b; Identification of cross-reactive and serotype 2-specific neutralization epitopes on VP3 of human rotavirus. Journal of Virology 62:2421–2426
    [Google Scholar]
  36. Taniguchi K., Nishikawa K., Urasawa T., Urasawa S., Midthun K., Kapikian A. Z., Gorziglia M. 1989; Complete nucleotide sequence of the gene encoding VP4 of a human rotavirus (strain K8) which has unique VP4 neutralization epitopes. Journal of Virology 63:4101–4106
    [Google Scholar]
  37. Urasawa S., Urasawa T., Taniguchi K. 1986; Genetic reassortment between two human rotaviruses having different serotype and subgroup specificities. Journal of General Virology 67:1551–1559
    [Google Scholar]
  38. Urasawa S., Urasawa T., Taniguchi K., Wakasugi F., Kobayashi N., Chiba S., Sakurada N., Morita M., Morita O., Tokieda M., Kawamoto H., Minekawa Y., Ohseto M. 1989; Survey of human rotavirus serotypes in different locales in Japan by enzyme-linked immunosorbent assay with monoclonal antibodies. Journal of Infectious Diseases 160:44–51
    [Google Scholar]
  39. Urasawa T., Urasawa S., Taniguchi K. 1981; Sequential passages of human rotavirus in MA-104 cells. Microbiology and Immunology 25:1025–1035
    [Google Scholar]
  40. Urasawa T., Urasawa S., Taniguchi K., Kobayashi N. 1988; Preparation of a human rotavirus reassortant with dual serotype specificity, VP3 of serotype 4 and VP7 of serotype 3. Sapporo Medical Journal 57:373–378
    [Google Scholar]
  41. Vesikari T., Isolauri E., D’Hondt E., Delem A., André F. E., Zissis G. 1984; Protection of infants against rotavirus diarrhoea by RIT 4237 attenuated bovine rotavirus strain vaccine. Lancet i:977–981
    [Google Scholar]
  42. Ward R. L., Knowlton D. R., Schiff G. M., Hoshino Y., Greenberg H. B. 1988; Relative concentrations of serum neutralizing antibody to VP3 and VP7 proteins in adults infected with a human rotavirus. Journal of Virology 62:1543–1549
    [Google Scholar]
  43. Wyatt R. G., Greenberg H. B., James W. D., Pittman A. L., Kalica A. R., Flores J., Chanock R. M., Kapikian A. Z. 1982; Definition of human rotavirus serotypes by plaque reduction assay. Infection and Immunity 37:110–115
    [Google Scholar]
  44. Yewdell J. W., Gerhard W. 1981; Antigenic characterization of viruses by monoclonal antibodies. Annual Review of Microbiology 35:185–206
    [Google Scholar]
  45. Yoshino K., Taniguchi S. 1965; Studies on the neutralization of herpes simplex virus. III. Mechanism of the antibody-potentiating action of complement. Virology 26:61–72
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-11-2615
Loading
/content/journal/jgv/10.1099/0022-1317-71-11-2615
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error