1887

Abstract

Cell fusion proceeded gradually in measles virus-infected cells incubated at 35 °C. Shift-up of incubation temperature to 39 °C induced rapidly increased cell fusion in spite of the cessation of synthesis of the fusion (F) protein. Pulse-chase experiments showed that there was little difference in the acquisition of immunoreactivity by haemagglutinin (H) and F proteins between the two temperatures. H protein was detected on the cell surface 60 min after the chase at either temperature. However, appearance of F protein on the cell surface took less than 3 h at 39 °C whereas it took 5 h at 35 °C. These data indicate that temperature elevation induces more efficient expression of F protein on the cell surface accompanied by marked syncytium formation in measles virus-infected cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-10-2475
1990-10-01
2022-01-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/10/JV0710102475.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-10-2475&mimeType=html&fmt=ahah

References

  1. Enders J. F., McCarthy K., Mitus A., Cheatham W. J. 1959; Isolation of measles virus at autopsy in cases of giant-cell pneumonia without rash. New England Journal of Medicine 261:875–881
    [Google Scholar]
  2. Graves M., Silver S. M., Choppin P. W. 1978; Measles virus polypeptide synthesis in infected cells. Virology 86:254–263
    [Google Scholar]
  3. Gupta L. S., Goldstein G., Boyse E. A. 1977; Accessibility of plasma membrane antigens. Immunogenetics 5:379–387
    [Google Scholar]
  4. Mottet G., Tuffereau C., Roux L. 1986; Reduced temperature can block different glycoproteins at different steps during transport to the plasma membrane. Journal of General Virology 67:2029–2035
    [Google Scholar]
  5. Ogura H., Nakamura S. 1989; Replication of measles virus in Vero cells at elevated temperatures. Microbiology and Immunology 33:907–914
    [Google Scholar]
  6. Ogura H., Baczko K., Rima B. K., Ter Meulen V. 1987; Selective inhibition of translation of the mRNA coding for the measles virus membrane protein at elevated temperatures. Journal of Virology 61:472–479
    [Google Scholar]
  7. Ogura H., Rima B.K., Baczko K., Tas P., Ter Meulen V. 1988; Restricted synthesis of the fusion protein of measles virus at elevated temperatures. Journal of General Virology 69:925–929
    [Google Scholar]
  8. Reeve P., Poste G. 1971; Studies on the cytopathogenicity of Newcastle disease virus: relation between virulence, polykario-cytosis and plaque size. Journal of General Virology 11:17–24
    [Google Scholar]
  9. Richardson C., Hull D., Greer P., Hasel K., Berkovich A., Englund G., Bellini W., Rima B., Lazzarini R. 1986; The nucleotide sequence of the mRNA encoding the fusion protein of measles virus (Edmonston strain): a comparison of fusion proteins from several different paramyxoviruses. Virology 155:508–523
    [Google Scholar]
  10. Sato T. A., Kohama T., Sugiura A. 1988; Intracellular processing of measles virus fusion protein. Archives of Virology 98:39–50
    [Google Scholar]
  11. Yoshida T., Nagai Y., Maeno K., Iinuma M., Hamaguchi M., Matsumoto T., Nagayoshi S., Hoshino M. 1979; Studies on the role of M protein in virus assembly using a ts mutant of HVJ (Sendai virus). Virology 92:139–154
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-10-2475
Loading
/content/journal/jgv/10.1099/0022-1317-71-10-2475
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error