1887

Abstract

The glycoprotein complex gp58/116 of human cytomegalovirus (HCMV) represents a dominant antigen for the humoral immune response. We have used the human monoclonal antibody C23, which is capable of neutralizing HCMV in tissue culture without the addition of complement, to study the origin of gp116 as well as the amino acid sequence recognized by the antibody. Our results show that gp116 is derived from the same open reading frame as gp58 and that it represents the amino-terminal portion of the precursor protein. Using prokaryote-expressed β-galactosidase-gp116 fusion proteins, the binding site of C23 was located to between amino acids 27 to 84 of the amino-terminal portion of gp116. Analyses of HCMV-positive human sera revealed that this portion of the molecule is immunogenic during natural infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-10-2443
1990-10-01
2021-10-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/10/JV0710102443.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-10-2443&mimeType=html&fmt=ahah

References

  1. Baboonian C., Blake K., Booth J. C., Wiblin C. N. 1989; Complement-independent neutralising monoclonal antibody with differential reactivity for strains of human cytomegalovirus. Journal of Medical Virology 29:139–145
    [Google Scholar]
  2. Bowden R. A., Sayers M., Flournoy N. 1986; Cytomegalovirus immune globulin and seronegative blood products to prevent primary cytomegalovirus infection after bone marrow transplantation. New England Journal of Medicine 314:1006–1010
    [Google Scholar]
  3. Britt W. J. 1984; Neutralizing antibodies detect a disulfide-linked glycoprotein complex within the envelope of human cytomegalovirus. Virology 135:369–378
    [Google Scholar]
  4. Britt W. J., Auger D. 1986; Synthesis and processing of the envelope gp55-116 complex of human cytomegalovirus. Journal of Virology 61:185–191
    [Google Scholar]
  5. Britt W. J., Vugler L. G. 1989; Processing of the gp55-116 envelope glycoprotein complex (gB) of human cytomegalovirus. Journal of Virology 63:403–410
    [Google Scholar]
  6. Britt W. J., Vugler L., Stephens E. B. 1988; Induction of complement dependent and independent neutralizing antibodies by recombinant derived human cytomegalovirus gp55-116 (gB). Journal of Virology 62:3309–3318
    [Google Scholar]
  7. Britt W. J., Vugler L., Butfiloski E. S., Stephans E. B. 1990a; Cell surface expression of human cytomegalovirus (HCMV) gp55- 116(gB): use of HCMV-vaccinia recombinant virus infected cells in analysis of the human neutralizing antibody response. Journal of Virology in press
    [Google Scholar]
  8. Britt W. J., Fay J., Stephens E., Kniess N., Utz U., Mach M. 1990b; Identification of an immunodominant linear epitope on human cytomegalovirus gp55/116 (gB). In Vaccines ′90 New York: Cold Spring Harbor Laboratory; in press
    [Google Scholar]
  9. Cranage M. P., Kouzarides T., Bankier A. T., Satchwell S., Weston K., Tomlinson P., Barrell B., Hart H., Bell S. E., Minson S. E., Smith G. L. 1986; Identification of the human cytomegalovirus glycoprotein B gene and induction of neutralizing antibodies via its expression in recombinant vaccinia virus. EMBO Journal 5:3057–3063
    [Google Scholar]
  10. Edson C. M., Hosler B. A., Waters D. J. 1987; Varicella zoster virus gpl and herpes simplex virus gE: phosphorylation and Fc binding. Virology 161:509–602
    [Google Scholar]
  11. Gabel C. A., Dubey L., Steinberg S. P., Sherman D., Gershon M. D., Gershon A. A. 1989; Varicella-zoster virus glycoprotein oligosaccharides are phosphorylated during posttranslational maturation. Journal of Virology 63:4264–4276
    [Google Scholar]
  12. Gong M., Ooka T., Matsuo T., Kieff E. 1987; Epstein-Barr virus glycoprotein homologous to herpes simplex virus gB. Journal of Virology 61:499–508
    [Google Scholar]
  13. Gretch D. R., Gehrz R. C., Stinski M. F. 1988; Characterization of a human cytomegalovirus glycoprotein complex (gel). Journal of General Virology 69:1205–1215
    [Google Scholar]
  14. Harlow E., Lane D. 1988 Antibodies: A laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  15. Hope R. G., Palfreyman J., Suh M., Mardsen H. S. 1982; Sulphated glycoproteins induced by herpes simplex virus. Journal of General Virology 58:399–415
    [Google Scholar]
  16. Houghten R. A. 1987; Facile determination of exact amino acid involvement in peptide antigen-monoclonal antibody interactions. In Vaccines ′87 pp 1–6 Chanock R., Lerner R., Brown F., Ginsberg H. Edited by New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Kari B., Lussenhop N., Goertz R., Wabuke-Bunoti M., Radeke R., Gehrz R. 1986; Characterization of monoclonal antibodies reactive to several biochemically distinct human cytomegalovirus glycoprotein complexes. Journal of Virology 60:345–352
    [Google Scholar]
  18. Kornfeld R., Kornfeld S. 1985; Assembly of asparagine-linked oligosaccharides. Annual Review of Biochemistry 54:631–664
    [Google Scholar]
  19. Kyte J., Doolittle R. F. 1982; A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology 157:105–132
    [Google Scholar]
  20. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  21. Lehner R., Meyer H., Mach M. 1989; Identification and characterization of a human cytomegalovirus gene coding for a membrane protein that is conserved among human herpesviruses. Journal of Virology 63:3792–3800
    [Google Scholar]
  22. Mach M., Utz U., Fleckenstein B. 1986; Mapping of the major glycoprotein gene of human cytomegalovirus. Journal of General Virology 67:1461–1467
    [Google Scholar]
  23. Mach M., Stamminger T., Jahn G. 1989; Human cytomegalovirus: recent aspects from molecular biology. Journal of General Virology 70:3117–3146
    [Google Scholar]
  24. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Masuho Y., Matsumoto Y.-I., Sugano T., Fujinaga S., Minamishima Y. 1987; Human monoclonal antibodies neutralizing human cytomegalovirus. Journal of General Virology 68:1457–1461
    [Google Scholar]
  26. Mirolo G., Baldassarri B., Ripalti A., Re M. C., Clementi M., Manzin A., Landini M. P. 1987; Antibody response to individual cytomegalovirus structural proteins in different groups of subjects. European Journal of Clinical Microbiology 6:207–210
    [Google Scholar]
  27. Montalvo E. A., Grose C. 1987; Assembly and processing of the disulfide-linked varicella-zoster virus glycoprotein gpll(140). Journal of Virology 61:2877–2884
    [Google Scholar]
  28. Pereira. L., Hoffman. M., Tatsuno. M., Dondero. D. 1984; Polymorphism of human cytomegalovirus glycoproteins characterized by monoclonal antibodies. irology 139:73–86
    [Google Scholar]
  29. Pereira L., Ali M., Kousoulas K., Huo B., Banks T. 1989; Domain structure of herpes simplex virus 1 glycoprotein B: neutralizing epitopes map in regions of continuous and discontinuous residues. Virology 172:11–24
    [Google Scholar]
  30. Perlman D., Halvorson H. O. 1983; A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptides. Journal of Molecular Biology 167:391–409
    [Google Scholar]
  31. Rasmussen L., Mullenax J., Nelson R., Merigan T. 1985; Viral polypeptides detected by complement-dependent neutralizing murine monoclonal antibody to human cytomegalovirus. Journal of Virology 55:274–280
    [Google Scholar]
  32. Rasmussen L., Nelson M., Neff M., Merigan T. 1988; Characterization of two different human cytomegalovirus glycoproteins which are targets for virus neutralizing antibody. Virology 163:308–318
    [Google Scholar]
  33. Schoofs P. G., Geysen H. M., Jackson D. C., Brown L. E., Tang X.-L., White D. O. 1988; Epitopes of an influenza viral peptide recognized by antibody at single amino acid resolution. Journal of Immunology 140:611–616
    [Google Scholar]
  34. Snydman D. R., Werner B. G., Heinze-Lacey B. 1987; Use of cytomegalovirus immune globulin to prevent cytomegalovirus disease in renal-transplant recipients. New England Journal of Medicine 317:1049–1054
    [Google Scholar]
  35. Spaete R. R., Thayer R. M., Probert W. S., Masiarz F. R., Chamberlain S. H., Rasmussen L., Merigan T. C., Pachl C. 1988; Human cytomegalovirus strain Towne glycoprotein B is processed by proteolytic cleavage. Virology 167:207–225
    [Google Scholar]
  36. Spear P. 1985; Glycoproteins specified by herpes simplex viruses. In The Herpesviruses 3 pp 315–356 Roizman B. Edited by London: Plenum Press;
    [Google Scholar]
  37. Stannard L. M., Rider J. R., Farrar G. H. 1989; Morphology and distribution of gp52 on extracellular human cytomegalovirus (HCMV) supports biochemical evidence that it represents the HCMV glycoprotein B. Journal of General Virology 70:1553–1560
    [Google Scholar]
  38. Utz U., Britt W., Vugler L., Mach M. 1989; Identification of a neutralizing epitope on glycoprotein gp58 of human cytomegalovirus. Journal of Virology 63:1995–2001
    [Google Scholar]
  39. WuDunn D., Spear P. G. 1989; Initial interaction of herpes simplex virus with cells is binding to heparan sulfate. Journal of Virology 63:52–58
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-10-2443
Loading
/content/journal/jgv/10.1099/0022-1317-71-10-2443
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error