1887

Abstract

The gene encoding the pseudorabies virus glycoprotein gp50 was cloned at the very left end of the genome of adenovirus type 5 to give a recombinant adenovirus (Ad-gp50) defective for the E1A gene. Ad-gp50 expressed high levels of gp50 in cells which either complemented (293 cells) or did not complement (Vero and HeLa cells) the E1A gene. Surprisingly, over an extended period, higher levels of gp50 were produced in HeLa cells which lack the E1A gene. Rabbits and mice inoculated with Ad-gp50 showed a strong antibody response against gp50. Some of them were protected from a virulent challenge with pseudorabies virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-10-2425
1990-10-01
2022-11-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/10/JV0710102425.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-10-2425&mimeType=html&fmt=ahah

References

  1. Baer G. M., Brooks R. C., Foggin C. M. 1989; Oral vaccination of dogs fed canine adenovirus in baits. American Journal of Veterinary Research 50:836–837
    [Google Scholar]
  2. Ballay A., Levrero M., Buendia M. A., Tiollais P., Perricaudet M. 1985; In vitro and in vivo synthesis of the hepatitis B surface antigen and of the receptor for polymerised human serum albumin from recombinant human adenoviruses. EMBO Journal 4:3861–3865
    [Google Scholar]
  3. Ballay A., Levrero M., Tiollais P., Perricaudet M. 1987; A hepatitis B adenovirus recombinant as a potential live vaccine. In Hepadnaviruses. UCLA Symposia on Molecular and Cellular Biology, New Series 70 pp 481–493 Robinson W., Koike K., Will H. Edited by New York: Alan R. Liss;
    [Google Scholar]
  4. Berget S. M., Moore C., Sharp P. 1977; Spliced RNA segments at the 5ʹ terminus of adenovirus 2 late mRNA. Proceedings of the National Academy of Sciences U.S.A.: 743171–3175
    [Google Scholar]
  5. Berkner K. L. 1988; Development of adenovirus vectors for the expression of heterologous genes. Biotechniques 6:616–629
    [Google Scholar]
  6. Betts A. O., Jennings A. R., Lamont P. H., Page Z. 1962; Inoculation of pigs with adenoviruses of man. Nature; London: 19345–46
    [Google Scholar]
  7. Chappuis G., Fargeaud D., Brun A. 1989; Industrial production and control of a subunit vaccine against Aujeszkyʹs disease. In Vaccination and Control of Aujeszkyʹs Disease pp 67–78 Van Oirschot J. T. Edited by Brussels & Luxembourg: ECSC, EEC, EAEC;
    [Google Scholar]
  8. Dermott M. R., Graham F. L., Hanke T., Johnson D. C. 1989; Protection of mice against lethal challenge with herpes simplex virus by vaccination with an adenovirus vector expressing HSV glyco-protein B. Virology 169:244–247
    [Google Scholar]
  9. Eloit M., Fargeaud D., LʹHaridon R., Toma B. 1988; Identification of the pseudorabies glycoprotein gp50 as a major target of neutralizing antibodies. Archives of Virology 99:45–56
    [Google Scholar]
  10. Eloit M., Fargeaud D., Vannier P., Toma B. 1989; Development of an ELISA test to differentiate between animals either vaccinated with or infected by Aujeszkyʹs disease virus. Veterinary Record 124:91–94
    [Google Scholar]
  11. Ghosh-Choudhury G., Haj-Ahmad Y., Graham F. L. 1987; Protein IX, a minor component of the human adenovirus capsid, is essential for the packaging of full length genomes. EMBO Journal 6:1733–1739
    [Google Scholar]
  12. Graham F. L., Van Der Eb A. J. 1973; A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456–467
    [Google Scholar]
  13. Graham F. L., Smiley J., Russell W. C., Nairn R. 1977; Characteristics of a human cell line transformed by DNA from human adenovirus type 5. Journal of General Virology 36:59–72
    [Google Scholar]
  14. Grand R. J. A. 1987; The structure and functions of the adenovirus early region I proteins. Biochemical Journal 241:25–38
    [Google Scholar]
  15. Ishii H., Kobayashi Y., Kuroki M., Kodama Y. 1988; Protection of mice from lethal infection with Aujeszkyʹs disease virus by immunization with purified gVI. Journal of General Virology 69:1411–1414
    [Google Scholar]
  16. Johnson D. C., Ghosh-Choudhury G., Smiley J. R., Fallis L., Graham F. L. 1988; Abundant expression of herpes simplex virus glycoprotein gB using an adenovirus vector. Virology 164:1–14
    [Google Scholar]
  17. Kost T. A., Jones E. V., Smith K. M., Reed A. P., Brown A. L., Miller J. J. 1989; Biological evaluation of glycoproteins mapping to two distinct mRNAs within the Bam HI fragment 7 of pseudorabies virus: expression of the coding region by vaccinia virus. Virology 171:365–376
    [Google Scholar]
  18. Levrero M., Ballay A., Schellekens H., Tiollais P., Perricaudet M. 1988; Hepatitis B adenovirus recombinant as a potential live vaccine. In Proceedings oj the 8th International Biotechnology Symposium, Paris pp 702–712 Durand G., Bobichon L., Durand L., Florent J. Edited by
    [Google Scholar]
  19. Lubeck M. D., Davis A. R., Chengalva M., Natuk R. J., Morin J. E., Molnar-Kimber K., Mason B. B., Bhat B. M., Mizutani S., Hung P. P., Purcell R. H. 1989; Immunogenicity and efficacy testing in chimpanzees of an oral hepatitis B vaccine based on live recombinant adenovirus. Proceedings of the National Academy of Sciences U.S.A.: 866763–6767
    [Google Scholar]
  20. Marchioli C. C., Yancey R. J., Petrovskis E. A., Timmins J. G., Post L. E. 1987; Evaluation of pseudorabies virus glycoprotein gp50 as a vaccine for Aujeszkyʹs disease in mice and swine: expression by vaccinia virus and Chinese hamster ovary cells. Journal of Virology 61:3977–3982
    [Google Scholar]
  21. Morin J. E., Lubeck M. P., Barton J. E., Conley A. J., Davis A. R., Hung P. P. 1987; Recombinant adenovirus induces antibody response to hepatitis B virus surface antigen in hamsters. Proceedings of the National Academy of Sciences U.S.A.: 844626–4630
    [Google Scholar]
  22. Murphy F. A. 1989; Vaccinia-vectored vaccines-risks and benefits. Research in Virology 140:463–491
    [Google Scholar]
  23. Nevins J. R. 1987; Regulation of early adenovirus gene expression. Microbiological Reviews 51:419–430
    [Google Scholar]
  24. Petrovskis E. A., Timmins J. G., Armentrout M. A., Marchioli C. C., Yancey R. J., Post L. E. 1986; DNA sequence of the gene for pseudorabies virus gp50, a glycoprotein without V-linked glycosylation. Journal of Virology 59:216–223
    [Google Scholar]
  25. Prevec L., Schneider M., Rosenthal K. L., Belbeck L. W., Derbyshire J. B., Graham F. L. 1989; Use of human adenovirus-based vectors for antigen expression in animals. Journal of General Virology 70:429–434 erratum 2539
    [Google Scholar]
  26. Schneider M., Graham F. L., Prevec L. 1989; Expression of the glycoprotein of vesicular stomatitis virus by infectious adenovirus vectors. Journal of General Virology 70:417–427
    [Google Scholar]
  27. Shenk T., Williams J. 1984; Genetic analysis of adenovirus. Current Topics in Microbiology and Immunology 111:1–39
    [Google Scholar]
  28. Sumner J. W., Shaddock H. H., Wu G., Baer G. M. 1988; Oral administration of an attenuated strain of canine adenovirus (type 2) to raccoons, foxes, skunk, and mongoose. American Journal of Veterinary Research 49:169–171
    [Google Scholar]
  29. Thummel C., Than R., Hu S. H., Grodzicker T. 1983; Translational control of adenovirus late promoter. Cell 33:455–464
    [Google Scholar]
  30. Wathen L. M. K., Plat K. B., Wathen M. W., Van Densen R. A., Whestone C. A., Pirtle E. C. 1985; Production and characterization of monoclonal antibody directed against pseudorabies virus. Virus Research 4:19–29
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-10-2425
Loading
/content/journal/jgv/10.1099/0022-1317-71-10-2425
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error