1887

Abstract

The high glycoprotein (gp300) of equine herpesvirus type 1 was found to have an , estimated by SDS-PAGE, of over 400000 and was confirmed as being a surface glycoprotein by I-labelling. In contrast to [H]glucosamine, gp300 showed very low levels of [H]mannose incoporation. The of gp300 showed no detectable change upon treatment of purified virus with (-glycanase, and showed only a small change in virus-infected cells treated with tunicamycin. In addition, gp300 failed to bind the lectin concanavalin A. Taken together, these results indicate a lack of -linked carbohydrate on gp300. The major carbohydrate species were found to be composed primarily of -linked chains, as indicated by the sensitivity of the protein to monensin, to exoglycanase enzymes specific for sugars present in -linked chains and to mild alkaline borohydride treatment, which revealed three species of carbohydrate of of > 10000, 2400 and 1100, respectively. Neuraminidase treatment and binding of lectin indicated the presence of α- -acetylglucosamine and sialic acid as terminal sugars. Immunological cross-reactivity of gp300 with a high protein of equine herpesvirus type 4 was shown and it also exhibited a marked variation in the vaccine strain Rhinomune.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-10-2407
1990-10-01
2024-12-13
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/10/JV0710102407.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-10-2407&mimeType=html&fmt=ahah

References

  1. Abodeely R. A., Palmer E., Lawson L. A., Randall C. C. 1971; The proteins of enveloped and de-enveloped equine abortion (herpes) virus and the separated envelope. Virology 44:146–152
    [Google Scholar]
  2. Allen G. P., Bryans J. T. 1986; Molecular epizootiology, pathogenesis and prophylaxis of equine herpesvirus-1 infections. Progress in Veterinary Microbiology and Immunology 2:78–144
    [Google Scholar]
  3. Allen G. P., Coogle L. D. 1988; Characterization of an equine herpesvirus type 1 gene encoding a glycoprotein (gpl3) with homology to herpes simplex virus glycoprotein C. Journal of Virology 62:2850–2858
    [Google Scholar]
  4. Allen G. P., Yeargen M. R. 1987; Use of λgtl 1 and monoclonal antibodies to map the genes for the six major glycoproteins of equine herpesvirus 1. Journal of Virology 61:2454–2461
    [Google Scholar]
  5. Bass E. P., Sharpee R. L., Beckenhauer W. H. 1973; Equine viral rhinopneumonitis: pathography and prophylaxis. Norden News 48:5–9
    [Google Scholar]
  6. Edington N., Bridges C. G., Broad S. C. 1987; Rapid diagnosis and characterization of equine herpesvirus-1 using monoclonal antibodies. Tierarztliche Praxis Supplement 2:37–40
    [Google Scholar]
  7. Edson C. M., Thorley-Lawson D. A. 1983; Synthesis and processing of the three major envelope glycoproteins of Epstein-Barr virus. Journal of Virology 46:547–556
    [Google Scholar]
  8. Elbein A. D. 1987; Inhibitors of the biosynthesis and processing of N-linked oligosaccharide chains. Annual Review of Biochemistry 56:497–534
    [Google Scholar]
  9. Florman H. M., Wasserman P. M. 1985; O-linked oligosaccharides of mouse egg ZP3 account for its sperm receptor activity. Cell 4l:313–324
    [Google Scholar]
  10. Goldstein I. J., Hayes C. E. 1978; The lectins: carbohydratebinding proteins of plants and animals. Advances in Carbohydrate Chemistry and Biochemistry 35:128–340
    [Google Scholar]
  11. Guo P., Goebel S., Davis S., Perteus M. E., Lanquet B., Desmettre P., Allen G., Paoletti E. 1989; Expression in recombinant vaccinia virus of the equine herpesvirus 1 gene encoding glycoprotein gpl3 and protection of immunized animals. Journal of Virology 63:4189–4198
    [Google Scholar]
  12. Hammarstrom S., Kabat E. A. 1969; Purification and characterization of a blood-group A reactive hemagglutinin from the snail Helixpomatia and a study of its combining site. Biochemistry 8:2696–2704
    [Google Scholar]
  13. Hammarstrom S., Murphy L. A., Goldstein I. J., Etzler M. E. 1977; Carbohydrate binding specificity of four N-acetyl-d-galacto-samine-“specific” lectins: Helix pomatia A hemagglutinin, soybean agglutinin, lima bean lectin and Dolichos biflorus lectin. Biochemistry 16:2750–2755
    [Google Scholar]
  14. Hancock K., Tsang V. C. W. 1983; India ink staining of protein on nitrocellulose paper. Analytical Biochemistry 133:157–162
    [Google Scholar]
  15. Heine J. W., Honess R. W., Cassai E., Roizman B. 1974; Proteins specified by herpes simplex virus XII. The virion polypeptides of type 1 strains. Journal of Virology 14:640–651
    [Google Scholar]
  16. Herp A., Wu A. M., Moschera J. 1979; Current concepts of the structure and nature of mammalian salivary mucous glycoproteins. Molecular and Cellular Biochemistry 23:27–43
    [Google Scholar]
  17. Holmes K., Doller E. W., Sturman L. S. 1981; Tunicamycin resistant glycosylation of a coronavirus glycoprotein: demonstration of a novel type of virus glycoprotein. Virology 115:334–344
    [Google Scholar]
  18. Hook M., Kjellen L., Johansson S., Robinson J. 1984; Cell- surface glycosaminoglycans. Annual Review of Biochemistry 53:847–869
    [Google Scholar]
  19. Iyer R. N., Carlson D. M. 1971; Alkaline borohydride degradation of blood group H substance. Archives of Biochemistry and Biophysics 142:101–105
    [Google Scholar]
  20. Jackson S. P., Tuan R. 1988; O-giycosylation of eukaryotic transcription factors: implications for mechanisms of transcriptional regulation. Cell 55:125–133
    [Google Scholar]
  21. Johnson D. C., Spear P. G. 1982; Monensin inhibits the processing of herpes simplex glycoproteins, their transport to the cell surface, and the egress of virions from infected cells. Journal of Virology 43:1101–1112
    [Google Scholar]
  22. Johnson D. C., Spear P. G. 1983; O-linked oligosaccharides are acquired by herpes simplex virus glycoproteins in the Golgi apparatus. Cell 32:987–997
    [Google Scholar]
  23. Kemp M. C., Perdue M. L., Rogers H. W., O’Callaghan D., Randall C. W. 1974; Structural polypeptides of the hamster strain of equine herpes virus type-1: products associated with purification. Virology 61:361–375
    [Google Scholar]
  24. Killington R. A., Yeo J., Honess R. W., Watson D. H., Duncan B. E., Halliburton I. W., Mumford J. 1977; Comparative analyses of the proteins and antigens of five herpesviruses. Journal of General Virology 37:297–310
    [Google Scholar]
  25. Killington R. A., Newhook L., Balachandran N., Rawls W. E., Bacchetti S. 1981; Production of hybrid cell lines secreting antibodies to herpes simplex virus type 2. Journal of Virological Methods 2:223–236
    [Google Scholar]
  26. Kornfield R., Kornfield S. 1980; Structure of glycoproteins and their oligosaccharide units. In The Biochemistry of Glycoproteins and Proteolgycans pp 1–34 Lennarz W. J. Edited by New York: Plenum Press;
    [Google Scholar]
  27. Lambert D. M. 1988; Role of oligosaccharides in the structure and function of respiratory syncytial virus glycoproteins. Virology 164:458–466
    [Google Scholar]
  28. Laskey R. A., Mills A. D. 1975; Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. European Journal of Biochemistry 56:335–341
    [Google Scholar]
  29. Ledger P. W., Tanzer M. L. 1984; Monensin - a pertubant of cellular physiology. Trends in Biochemical Sciences 313:314
    [Google Scholar]
  30. McGeoch D. J., Moss H. W. M., McNab D., Frame M. C. 1987; DNA sequence and genetic content of the HindIII l region in the short unique component of the herpes simplex virus type 2 genome: identification of the gene encoding glycoprotein G, and evolutionary comparisons. Journal of General Virology 68:19–38
    [Google Scholar]
  31. Maley F., Trimble R. B., Tarentino A. L., Plummer T. H. 1989; Characterization of glycoproteins and their associated oligosaccharides through the use of endoglycosidases. Analytical Biochemistry 180:195–204
    [Google Scholar]
  32. Marsden H. S., Buckmaster A., Palfreyman J. W., Hope R. G., Minson A. C. 1984; Characterization of the 92,000-dalton glycoprotein induced by herpes simplex virus type 2. Journal of Virology 50:547–554
    [Google Scholar]
  33. Meredith D. M., Stocks J.-M., Whittaker G. R., Halliburton I. W., Snowden B. W., Killington R. A. 1989; Identification of the gB homologues of equine herpesvirus types 1 and 4 as disulphide-linked heterodimers and their characterization using monoclonal antibodies. Journal of General Virology 70:1161–1172
    [Google Scholar]
  34. Niemann H., Klenk H. D. 1981; Coronavirus glycoprotein El, a new type of viral glycoprotein. Journal of Molecular Biology 153:993–1010
    [Google Scholar]
  35. Niemann H., Geyer R., Klenk H. D., Linder D., Stirm S., Wirth M. 1984; The carbohydrates of mouse hepatitis virus (MHV) A59: structures of the O-glycosidically linked oligosaccharides of glycoprotein El. EMBO Journal 3:665–670
    [Google Scholar]
  36. Ogato S. I., Lloyd K. O. 1982; Mild alkaline borohydride treatment of glycoproteins - a method for liberating both N- and O-linked carbohydrate chains. Analytical Biochemistry 119:351–359
    [Google Scholar]
  37. Olofsson S., Jeansson S., Lycke E. 1981; Unusual lectin-binding properties of a herpes simplex virus type 1-specific glycoprotein. Journal of Virology 38:564–570
    [Google Scholar]
  38. Olofsson S., Sjöblom I., Lundström M., Jeansson S., Lycke E. 1983; Glycoprotein C of herpes simplex virus type 1: characterization of O-linked oligosaccharides. Journal of General Virology 64:2735–2747
    [Google Scholar]
  39. Olofsson S., Lundström M., Marsden H., Jeansson S., Vahlne A. 1986; Characterization of a herpes simplex virus type 2-specified glycoprotein with affinity for N-acetylgalactosamine-specific lectins and its identification as g92K or gG. Journal of General Virology 67:737–744
    [Google Scholar]
  40. Perdue M. L., Kemp M. C., Randall C. C., O’Callaghan D. 1974; Studies of the molecular anatomy of the L-M cell strain of equine herpesvirus type 1: proteins of the nucleocapsid and intact virion. Virology 59:201–216
    [Google Scholar]
  41. Rasilo M. L., Renkonen O. 1981; Mild alkaline borohydride treatment liberates N-acetylglucosamine-linked oligosaccharide chains of glycoproteins. FEBS Letters 135:38–42
    [Google Scholar]
  42. Serafini-Cessi F., Malagolini N., Dall’olio F., Pereira L., Campadelli-Fiume G. 1985; Oligosaccharide chains of herpes simplex virus type 2 glycoprotein gG-2. Archives of Biochemistry and Biophysics 240:866–876
    [Google Scholar]
  43. Serafini-CESSI F., Dall’olio F., Malagolini N., Pereira L., Campadelli-Fiume G. 1988; Comparative study on O-linked oligosaccharides of glycoprotein D of herpes simplex virus types 1 and 2. Journal of General Virology 69:869–877
    [Google Scholar]
  44. Serafini-Cessi F., Malagolini N., Nanni M., Dall’Olio F., Campadelli-Fiume G., Tanner J., Kieff E. 1989; Characterization of N- and O-linked oligosaccharides of glycoprotein 350 from Epstein-Barr virus. Virology 170:1–10
    [Google Scholar]
  45. Shida H., Dales S. 1981; Biogenesis of vaccinia: carbohydrate of the hemagglutination molecule. Virology 111:556–72
    [Google Scholar]
  46. Sinclair R., Cook R. F., Mumford J. A. 1989; The characterization of neutralizing and non-neutralizing monoclonal antibodies against equid herpesvirus type 1. Journal of General Virology 70:455–459
    [Google Scholar]
  47. Tanner J., Weis J., Fearon D., Whang Y., Kieff E. 1987; Epstein-Barr virus gp 350/220 binding to B lymphocyte C3d receptor mediates adsorption, capping and endocytosis. Cell 50:203–213
    [Google Scholar]
  48. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets; procedure and some applications. Proceedings of the National Academy of Sciences U.S.A.: 764350–4354
    [Google Scholar]
  49. Turtinen L. W., Allen G. P. 1982; Identification of the envelope surface glycoproteins of equine herpesvirus type 1. Journal of General Virology 63:481–485
    [Google Scholar]
  50. Von Figura K. 1977; Human α-N-acetylglucosaminidase: 1- Purification and properties. European Journal of Biochemistry 80:525–533
    [Google Scholar]
  51. Wenske E. A., Courtney R. J. 1983; Glycosylation of herpes simplex virus type 1 gC in the presence of tunicamycin. Journal of Virology 46:297–301
    [Google Scholar]
  52. Wertz G. W., Collins P. L., Huang Y., Gruber C., Levine S. 1985; Nucleotide sequence of the G protein gene of human respiratory syncytial virus reveals an unusual type of viral membrane protein. Proceedings of the National Academy of Sciences U.S.A.: 824075–4079
    [Google Scholar]
  53. York J. J., Sonza S., Fahey K. J. 1987; Immunogenic glycoproteins of infectious laryngotracheitis herpesvirus. Virology 161:340–347
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-71-10-2407
Loading
/content/journal/jgv/10.1099/0022-1317-71-10-2407
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error