1887

Abstract

Polydnavirus from sp. (CcV) was purified and 18 structural polypeptides associated with CcV were identified by silver staining. Antibodies were raised against CcV protein and used in testing for the presence of the virus in different tissues of the wasp ovary and in stung eggs. It was also established that the virus does not enter during the first 5 s of oviposition. Furthermore, no degradation of the virus proteins was detected inside the egg within 2 h after oviposition. The glycoprotein nature of virus proteins was also determined by concanavalin A/horseradish peroxidase staining. The amino acid compositions of the most highly abundant peptides (41K, 33K, 21K, 17K and 13K) were determined, as was the N-terminal amino acid sequence of the 41K protein. The latter did not show similarity with any reported protein sequences.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-10-2353
1990-10-01
2022-08-11
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/10/JV0710102353.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-10-2353&mimeType=html&fmt=ahah

References

  1. Burnette W. N. 1981; “Western blotting” electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein. Analytical Biochemistry 112:195–197
    [Google Scholar]
  2. Blissard G. W., Smith O. P., Summers M. D. 1987; Two related viral genes are located on single superhelical DNA segment of the multipartite Campoletis sonorensis virus genome. Virology 160:120–134
    [Google Scholar]
  3. Blissard G. W., Theilmann D. A., Summers M. D. 1989; Segment W of Campoletis sonorensis virus: expression, gene products and organization. Virology 169:78–89
    [Google Scholar]
  4. Cook D., Stoltz D. B. 1983; Comparative serology of viruses isolated from ichneumonid parasitoids. Virology 130:215–220
    [Google Scholar]
  5. Edson K. M., Vinson S. B., Stoltz D. B., Summers M. D. 1981; Virus in a parasite wasp: suppression of the cellular immune response in the parasitoid’s host. Science 211:582–583
    [Google Scholar]
  6. Feddersen I., Sander K., Schmidt O. 1986; Virus-like particle with host protein-like antigenic determinant protect an insect parasite from encapsulation. Experientia 42:1278–1281
    [Google Scholar]
  7. Jones D. 1986; Chelonus sp. suppression of host ecdysteroids and developmentally stationary pseudoparasitized prepupae. Experimental Parasitology 161:10–12
    [Google Scholar]
  8. Jones D. 1987; Material from adult female Chelonus sp. directs expression of altered developmental program of host Lepidoptera. Journal of Insect Physiology 33:129–134
    [Google Scholar]
  9. Jones D., Sreekrishna S., Iwaya M., Yang J., Eberely M. 1986a; Comparison of viral ultrastructure and DNA banding patterns from the reproductive tracts of eastern and western hemisphere Chelonus sp. (Braconidae: Hymenoptera). Journal of Invertebrate pathology 47:105–115
    [Google Scholar]
  10. Jones D., Jones G., Rudnicka M., Click A., Malleczewen R., Iwaya M. 1986b; Pseudoparasitism of host Trichoplusia ni by Chelonus sp. as new model system for parasite regulation of host physiology. Journal of Insect Physiology 32:315–328
    [Google Scholar]
  11. Krell P. J. 1987; Replication of long virus-like particles in the reproductive tract of the ichneumonid wasp Diadegma terebrans . Journal of General Virology 68:1477–1483
    [Google Scholar]
  12. Krell P. J., Stoltz D. B. 1979; Unusual baculovirus of the parasite wasp Apanteles melanoscelus: isolation and preliminary characterization. Journal of Virology 29:1118–1130
    [Google Scholar]
  13. Krell P. J., Stoltz D. B. 1980; Virus-like particles in the ovary of an ichneumonid wasp: purification and preliminary characterization. Virology 101:408–418
    [Google Scholar]
  14. Krell P. J., Summers M. D., Vinson S. B. 1982; Virus with a multipartite superhelical DNA genome from the ichneumonid parasite Campoletis sonorensis . Journal of Virology 43:859–870
    [Google Scholar]
  15. Laemmli U. K. 1970; Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature; London: 227680–681
    [Google Scholar]
  16. Leluk J., Jones D. 1989; Chelonus sp. near curvimaculatus venom proteins: analysis of their potential role and processing during development of host Trichoplusia ni . Archives of Insect Biochemistry 10:1–12
    [Google Scholar]
  17. Leluk J., Schmidt J., Hones D. 1988; Characterization and functions of the proteins of hymenopteran venoms. In Endocrinological Frontiers in Physiological Insect Ecology 1 pp Sehnal F., Zabza A., Denlinger D. L. Edited by Wroclaw: Wroclaw Technical University Press;
    [Google Scholar]
  18. Lin R. C. 1986; Quantification of apolipoproteins in rat serum in cultured rat hepatocytes by enzyme linked immunosorbent assay. Analytical Biochemistry 154:316–317
    [Google Scholar]
  19. Matsudaira P. 1987; Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. Journal of Biological Chemistry 262:10035–10038
    [Google Scholar]
  20. Morrissey J. H. 1981; Silver stain of proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Analytical Biochemistry 117:307–310
    [Google Scholar]
  21. Stoltz D. B., Cook D. I. 1983; Inhibition of host phenoloxidase activity by parasitoid Hymenoptera. Experientia 39:1021–1022
    [Google Scholar]
  22. Stoltz D. B., Vinson S. B. 1979; Viruses and parasitism in insects. Advances in Virus Research 24:125–127
    [Google Scholar]
  23. Stoltz D. B., Summers M. D., Vinson S. B. 1984; Polydnaviri-dae: a proposed family of insect viruses with segmented doublestranded, circular DNA genomes. Intervirology 21:1–3
    [Google Scholar]
  24. Stoltz D. B., Guzo D., Belland E. R., Lucarotti C. J., Mackinnon E. A. 1988; Venom promotes uncoating in vitro and persistence in vivo of DNA from a braconid polydnavirus. Journal of General Virology 69:903–907
    [Google Scholar]
  25. Tejedor F., Ballesta J. P. G. 1982; Iodination of biological samples without loss of functional activity. Analytical Biochemistry 127:143–144
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-10-2353
Loading
/content/journal/jgv/10.1099/0022-1317-71-10-2353
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error