Restriction enzyme site mapping showed that most BamHI and all ClaI sites were conserved on the genomes of 17 African swine fever virus isolates from separate disease outbreaks that occurred between 1982 and 1989 in Malawi. However, frequent variation between virus genomes did occur due to addition or deletion of DNA sequences at various positions along the genome and 11 virus genotypes could thus be distinguished among the 17 isolates analysed. Length variations occurred at 10 different loci on the virus genome. These variable regions were located between the left DNA terminus and a position up to 48 kb from that terminus, in the centre of the genome 90 to 93 kb from the left DNA terminus and between the right DNA terminus and a position 22 kb from that terminus. Length variations in most of these regions were small (< 1 kb) but variations of about 4 kb occurred in a region up to 20 kb from the left DNA terminus.
AgueroM.,
BlascoR.,
WilkinsonP.,
ViñuelaE.1990; Analysis of naturally occurring deletion variants of African swine fever virus: multigene family 110 is not essential for infectivity or virulence in pigs. Virology 176:195–204
BlascoR.,
De La VegaI.,
AlmazanF.,
AgueroM.,
ViñuelaE.1989b; Genetic variation of African swine fever virus: variable regions near the ends of the viral DNA. Virology 173:251–257
DixonL. K.1988; Molecular cloning and restriction enzyme mapping of an African swine fever virus isolate from Malawi. Journal of General Virology 69:1683–1694
DixonL. K.,
WilkinsonP. J.1988; Genetic diversity of African swine fever virus isolates from soft ticks (Ornithodorus moubata)inhabiting warthog burrows in Zambia. Journal of General Virology 69:2981–2993
DixonL. K.,
WilkinsonP. J.,
SumptionK. J.,
EkueF.1990a; Diversity of the African swine fever virus genome. In Molecular Biology of Iridoviruses pp 271–295DaraiG.
Edited by Boston: Kluwer Academic;
DixonL. K.,
BristowC.,
WilkinsonP. J.,
SumptionK. J.1990b; Identification of a variable region of the African swine fever virus genome which has undergone separate DNA rearrangements leading to expansion of minisatellite-like sequences. Journal of Molecular Biology in press
FeinbergA. P.,
VogelsteinB.1983; A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 132:6–13
FeinbergA. P.,
VogelsteinB.1984; Addendum “A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity”. Analytical Biochemistry 137:266–267
GonzalezA.,
CalvoV.,
AlmazanF.,
AlmendralJ. M.,
RamirezJ. C.,
De La VegaI.,
BlascoR.,
ViñuelaE.1990; Multigene families in African swine fever: family 360. Journal of Virology 64:2073–2081
HaresnapeJ. M.,
WilkinsonP. J.1989; A study of African swine fever virus infected ticks (Omithodoros moubata)collected from three villages in the African swine fever enzootic area of Malawi following an outbreak of the disease in domestic pigs. Epidemiology and Infection 102:507–522
HaresnapeJ. M.,
WilkinsonP. J.,
MellorP. S.1988; Isolation of African swine fever virus from ticks of the Omithodoros moubatacomplex (Ixodoidea: Argasidae) collected within the African swine fever enzootic area of Malawi. Epidemiology and Infection 101:173–185
MossB.,
WintersE.,
CooperN.1981; Instability and reiteration of DNA sequences within the vaccinia virus genome. Proceedings of the National Academy of Sciences U.S.A.: 781614–1618
PlowrightW.1981; African swine fever. In Infectious Diseases of Wild Mammals, 2nd Edn. pp 178–190DavisJ. W.,
KarstadL. H.,
TrainerD. O.
Edited by Ames: Iowa State University Press;
SogoJ. M.,
AlmendralJ. M.,
TalaveraA.,
ViñuelaE.1984; Terminal and internal inverted repetitions in African swine fever virus DNA. Virology 133:271–275
ThomsonG. R.1985; The epidemiology of African swine fever: the role of free-living hosts in Africa. Onderstepoort Journal of Veterinary Research 52:201–209
ThomsonG.,
GainaruM.,
LewisA.,
BiggsH.,
NevillE.,
Van Der PypekampH.,
GerberL.,
EsterhuysenJ.,
BengisR.,
BezuidenhoutD.,
CondyJ.1983; The relationship between African swine fever virus, the warthog and Omithodoros species in Southern Africa. In African Swine Fever, CEC/FAO Research Seminar pp 85–100WilkinsonP. J.
Edited by Luxembourg: EUR 8466 EN;
WesleyR. D.,
TuthillA. E.1984; Genome relatedness among African swine fever virus field isolates by restriction endonuclease analysis. Preventive Veterinary Medicine 2:53–62
WilkinsonP. J.1981; African swine fever. In Virus Diseases of Food Animals. A World Geography of Epidemiology and Control2 Disease Monographs pp 767–786GibbsE. P. J.
Edited by London: Academic Press;