1887

Abstract

Restriction enzyme site mapping showed that most HI and all I sites were conserved on the genomes of 17 African swine fever virus isolates from separate disease outbreaks that occurred between 1982 and 1989 in Malawi. However, frequent variation between virus genomes did occur due to addition or deletion of DNA sequences at various positions along the genome and 11 virus genotypes could thus be distinguished among the 17 isolates analysed. Length variations occurred at 10 different loci on the virus genome. These variable regions were located between the left DNA terminus and a position up to 48 kb from that terminus, in the centre of the genome 90 to 93 kb from the left DNA terminus and between the right DNA terminus and a position 22 kb from that terminus. Length variations in most of these regions were small (< 1 kb) but variations of about 4 kb occurred in a region up to 20 kb from the left DNA terminus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-10-2331
1990-10-01
2022-08-10
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/10/JV0710102331.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-10-2331&mimeType=html&fmt=ahah

References

  1. Aguero M., Blasco R., Wilkinson P., Viñuela E. 1990; Analysis of naturally occurring deletion variants of African swine fever virus: multigene family 110 is not essential for infectivity or virulence in pigs. Virology 176:195–204
    [Google Scholar]
  2. Almendral J. M., Blasco R., Viñuela E. 1990; Multigene family in African swine fever virus DNA. Family 110. Journal of Virology 64:2064–2072
    [Google Scholar]
  3. Blasco R., Aguero M., Almendral J. M., Viñuela E. 1989a; Variable and constant regions in African swine fever virus DNA. Virology 168:330–338
    [Google Scholar]
  4. Blasco R., De La Vega I., Almazan F., Aguero M., Viñuela E. 1989b; Genetic variation of African swine fever virus: variable regions near the ends of the viral DNA. Virology 173:251–257
    [Google Scholar]
  5. Costa J. V. 1990; African swine fever virus. In Molecular Biology of Iridoviruses pp 247–270 Darai G. Edited by Boston: Kluwer Academic;
    [Google Scholar]
  6. Cox B. F., Hess W. R. 1962; Note on African swine fever investigation in Nyasaland. Bulletin of Epizootic Diseases ofAfrica 10:439–140
    [Google Scholar]
  7. Dixon L. K. 1988; Molecular cloning and restriction enzyme mapping of an African swine fever virus isolate from Malawi. Journal of General Virology 69:1683–1694
    [Google Scholar]
  8. Dixon L. K., Wilkinson P. J. 1988; Genetic diversity of African swine fever virus isolates from soft ticks (Ornithodorus moubata)inhabiting warthog burrows in Zambia. Journal of General Virology 69:2981–2993
    [Google Scholar]
  9. Dixon L. K., Wilkinson P. J., Sumption K. J., Ekue F. 1990a; Diversity of the African swine fever virus genome. In Molecular Biology of Iridoviruses pp 271–295 Darai G. Edited by Boston: Kluwer Academic;
    [Google Scholar]
  10. Dixon L. K., Bristow C., Wilkinson P. J., Sumption K. J. 1990b; Identification of a variable region of the African swine fever virus genome which has undergone separate DNA rearrangements leading to expansion of minisatellite-like sequences. Journal of Molecular Biology in press
    [Google Scholar]
  11. Ekue N. F. 1989 Epidemiology of African swine fever in Cameroon Ph.D. thesis University of Surrey:
    [Google Scholar]
  12. Feinberg A. P., Vogelstein B. 1983; A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 132:6–13
    [Google Scholar]
  13. Feinberg A. P., Vogelstein B. 1984; Addendum “A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity”. Analytical Biochemistry 137:266–267
    [Google Scholar]
  14. Gonzalez A., Calvo V., Almazan F., Almendral J. M., Ramirez J. C., De La Vega I., Blasco R., Viñuela E. 1990; Multigene families in African swine fever: family 360. Journal of Virology 64:2073–2081
    [Google Scholar]
  15. Haresnape J. M., Wilkinson P. J. 1989; A study of African swine fever virus infected ticks (Omithodoros moubata)collected from three villages in the African swine fever enzootic area of Malawi following an outbreak of the disease in domestic pigs. Epidemiology and Infection 102:507–522
    [Google Scholar]
  16. Haresnape J. M., Lungu S. A. M., Mamu F. D. 1985; A four-year survey of African swine fever in Malawi. Journal of Hygiene 95:309–323
    [Google Scholar]
  17. Haresnape J. M., Lungu S. A. M., Mamu F. D. 1987; An updated survey of African swine fever in Malawi. Epidemiology and Infection 99:723–732
    [Google Scholar]
  18. Haresnape J. M., Wilkinson P. J., Mellor P. S. 1988; Isolation of African swine fever virus from ticks of the Omithodoros moubatacomplex (Ixodoidea: Argasidae) collected within the African swine fever enzootic area of Malawi. Epidemiology and Infection 101:173–185
    [Google Scholar]
  19. Hess W. R. 1971; African swine fever virus. In Virology Monographs 9 pp 1–33 Vienna: Springer-Verlag;
    [Google Scholar]
  20. Leite Velho E. 1956; African swine fever in Angola. Bulletin de I′Office International des Epizooties 46:335–340
    [Google Scholar]
  21. Ley V., Almendral J. M., Carbonero P., Beloso A., Viñuela E., Talavera A. 1984; Molecular cloning of African swine fever virus DNA. Virology 133:249–257
    [Google Scholar]
  22. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Moss B., Winters E., Cooper N. 1981; Instability and reiteration of DNA sequences within the vaccinia virus genome. Proceedings of the National Academy of Sciences U.S.A.: 781614–1618
    [Google Scholar]
  24. Plowright W. 1981; African swine fever. In Infectious Diseases of Wild Mammals, 2nd Edn. pp 178–190 Davis J. W., Karstad L. H., Trainer D. O. Edited by Ames: Iowa State University Press;
    [Google Scholar]
  25. Plowright W., Parker J., Peirce M. A. 1969; African swine fever virus in ticks (Omithodorus moubata Murray) collected from animal burrows in Tanzania. Nature; London: 2211071–1072
    [Google Scholar]
  26. Sogo J. M., Almendral J. M., Talavera A., Viñuela E. 1984; Terminal and internal inverted repetitions in African swine fever virus DNA. Virology 133:271–275
    [Google Scholar]
  27. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  28. Thomson G. R. 1985; The epidemiology of African swine fever: the role of free-living hosts in Africa. Onderstepoort Journal of Veterinary Research 52:201–209
    [Google Scholar]
  29. Thomson G., Gainaru M., Lewis A., Biggs H., Nevill E., Van Der Pypekamp H., Gerber L., Esterhuysen J., Bengis R., Bezuidenhout D., Condy J. 1983; The relationship between African swine fever virus, the warthog and Omithodoros species in Southern Africa. In African Swine Fever, CEC/FAO Research Seminar pp 85–100 Wilkinson P. J. Edited by Luxembourg: EUR 8466 EN;
    [Google Scholar]
  30. Turnbull D. O. 1933 Report. Veterinary Department, Nyasaland. 1932
    [Google Scholar]
  31. Viñuela E. 1985; African swine fever virus. Current Topics in Microbiology and Immunology 116:151–170
    [Google Scholar]
  32. Viñuela E. 1987; African swine fever virus. In Developments in Veterinary Virology pp 31–49 Becker Y. Edited by The Hague: Martinus Nijhoff;
    [Google Scholar]
  33. Wesley R. D., Tuthill A. E. 1984; Genome relatedness among African swine fever virus field isolates by restriction endonuclease analysis. Preventive Veterinary Medicine 2:53–62
    [Google Scholar]
  34. Wilkinson P. J. 1981; African swine fever. In Virus Diseases of Food Animals. A World Geography of Epidemiology and Control 2 Disease Monographs pp 767–786 Gibbs E. P. J. Edited by London: Academic Press;
    [Google Scholar]
  35. Wilkinson P. J. 1984; The persistence of African swine fever in Africa and the Mediterranean. Preventive Veterinary Medicine 2:71–82
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-10-2331
Loading
/content/journal/jgv/10.1099/0022-1317-71-10-2331
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error