1887

Abstract

Reassortant viruses containing heterologous S and M genomic RNA segments were obtained from both mosquito and vertebrate hosts that had been co-infected with Egyptian and Senegalese strains of Rift Valley fever (RVF) virus. The origin of the S and M RNA segments in each plaque-cloned virus was determined with monoclonal antibodies capable of differentiating the nucleocapsid protein (S segment marker) or the G1 glycoprotein (M segment marker) of the parental strains. In the mosquito , reassortants were detected after sequential ingestion of parental viruses by interrupted feeding on two infected hamster hosts, after feeding on a single host that had been infected with both parental strains, and from individual mosquitoes inoculated intrathoracically with both parental strains. Reassortant viruses replicated efficiently in mosquitoes and were readily transmissible by bite to hamsters. Replication of a second infecting strain of RVF virus was, however, completely inhibited if that virus was inoculated into a mosquito ⩾48 h after the first viral strain. Genetic reassortment may provide a mechanism for increased heterogeneity, and thus affect the epidemiology and evolution of RVF virus.

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1990-10-01
2024-04-18
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References

  1. Beaty B. J., Bishop D. H. L., Gay M., Fuller F. 1983; Interference between bunyaviruses in Aedes triseriatus mosquitoes. Virology 127:83–90
    [Google Scholar]
  2. Beaty B. J., Sundin D. R., Chandler L. J., Bishop D. H. L. 1985; Evolution of bunyaviruses by genome reassortment in dually infected mosquitoes (Aedes triseriatus). Science 230:548–550
    [Google Scholar]
  3. Bishop D. H. L., Shope R. E. 1979; Bunyaviridae. In Comprehensive Virology 14 pp 1–132 Fraenkel-Conrat H., Wagner R. R. Edited by New York: Plenum Press;
    [Google Scholar]
  4. Buckler-White A. J., Murphy B. R., Naeve C. W. 1985; Characterization of the genes involved in host-range restriction of avian influenza-A viruses in primates. In Vaccines 85:Molecular and Chemical Basis of Resistance to Parasitic, Bacterial, and Viral Diseases pp 345–350 Lemer R. A., Chanock R. M., Brown F. Edited by New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  5. Caplen H., Peters C. J., Bishop D. H. L. 1985; Mutagen-directed attenuation of Rift Valley fever virus as a method for vaccine development. Journal of General Virology 66:2271–2277
    [Google Scholar]
  6. Davies C. R., Jones L. D., Green B. M., Nuttall P. A. 1987; In vivo reassortment of Thogoto virus (a tick-borne influenza-like virus) following oral infection of Rhipicephalus appendiculatus ticks. Journal of General Virology 68:2331–2338
    [Google Scholar]
  7. Gargan T. P.II Bailey C. L., Higbee G. A., Gad A., El Said S. 1983; The effect of laboratory colonization on the vector-pathogen interactions of Egyptian Culex pipiens and Rift Valley fever virus. American Journal of Tropical Medicine and Hygiene 32:1154–1163
    [Google Scholar]
  8. Jones L. D., Davies C. R., Green B. M., Nuttall P. A. 1987; Reassortment of Thogoto virus (a tick-borne influenza-like virus) in a vertebrate host. Journal of General Virology 68:1299–1306
    [Google Scholar]
  9. Meegan J. M. 1979; The Rift Valley fever epizootic in Egypt 1977–78. 1. Description of the epizootic and virological studies. Transactions of the Royal Society of Tropical Medicine and Hygiene 73:618–623
    [Google Scholar]
  10. Meegan J. M., Khalil G. M., Hoogstraal H., Adham F. K. 1980; Experimental transmission and field isolation studies implicating Culex pipiens as a vector of Rift Valley fever virus in Egypt. American Journal of Tropical Medicine and Hygiene 29:1405–1410
    [Google Scholar]
  11. Rosen L., Gubler D. 1974; The use of mosquitoes to detect and propagate dengue viruses. American Journal of Tropical Medicine and Hygiene 23:1153–1160
    [Google Scholar]
  12. Rossi C. A., Turell M. J. 1988; Characterization of attenuated strains of Rift Valley fever virus. Journal of General Virology 69:817–823
    [Google Scholar]
  13. Saluzzo J. F., Anderson G. W.Jr Hodgson L. A., Digoutte J. P., Smith J. F. 1989; Antigenic and biological properties of Rift Valley fever virus isolated during the 1987 Mauritanian epidemic. Research in Virology 140:155–164
    [Google Scholar]
  14. Saluzzo J. F., Smith J. F. 1990; Use of attenuated viruses to map attenuating and temperature-sensitive mutations of the Rift Valley fever virus MP-12 vaccine. Vaccine 8:369–375
    [Google Scholar]
  15. Turell M. J., Gargan T. P.II Bailey C. L. 1984; Replication and dissemination of Rift Valley fever virus in Culex pipiens. American Journal of Tropical Medicine and Hygiene 33:176–181
    [Google Scholar]
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