Full-length infectious DNA clones were constructed for four distinct phenotypic variants of beet curly top virus (BCTV). Southern hybridization assays indicated that each cloned BCTV genome shared sequence homology with pBCT-028, a full-length infectious DNA clone of a California isolate of BCTV previously characterized by others. Restriction endonuclease maps of the cloned BCTV genomes were distinct from one another. Infectivity assays determined that plasmids containing tandem repeats of BCTV genomes were generally more infectious than excised linear DNA inserts. Progeny virus, derived from plants inoculated with cloned DNAs, differed in their ability to infect sugarbeet, Beta vulgaris L., and the severity of symptoms produced in B. vulgaris and other experimental hosts.
GrimsleyN.,
HohnB.,
HohnT.,
WaldenR.1986; “Agroinfection”, an alternative route for viral infection of plants by using the Ti plasmid. Proceedings of the National Academy of Sciences U.S.A: 833282–3286
HayesR. J.,
BroughC. L.,
PrinceV. E.,
CouttsR. H. A.,
BuckK. W.1988; Infection of Nicotiana benthamiana with uncut cloned tandem dimers of tomato golden mosaic virus DNA. Journal of General Virology 69:209–218
LarsenR. C.,
DuffusJ. E.1984; A simplified procedure for purification of curly top virus and the isolation of its monomer and dimer particles. Phytopathology 74:114–118
MagyarosyA. C.,
DuffusJ. E.1977; The occurrence of highly virulent strains of the beet curly top virus in California. Plant Disease Reporter 61:248–250
MorinagaT.,
IkegamiM.,
AraiT.,
YazakiK.,
MiuraK.1988; Infectivity of cloned tandem dimer DNAs of bean golden mosaic virus. Journal of General Virology 69:879–902
StanleyJ.,
MarkhamP. G.,
CallisR. J.,
PinnerM. S.1986; The nucleotide sequence of an infectious clone of the geminivirus beet curly top virus. EMBO Journal 5:1761–1767
ThornleyW. R.,
MumfordD. L.1979; Intracellular location of beet curly top virus antigen as revealed by fluorescent antibody staining. Phytopathology 69:738–740
VieiraJ.,
MessingJ.1982; The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268