1887

Abstract

Full-length infectious DNA clones were constructed for four distinct phenotypic variants of beet curly top virus (BCTV). Southern hybridization assays indicated that each cloned BCTV genome shared sequence homology with pBCT-028, a full-length infectious DNA clone of a California isolate of BCTV previously characterized by others. Restriction endonuclease maps of the cloned BCTV genomes were distinct from one another. Infectivity assays determined that plasmids containing tandem repeats of BCTV genomes were generally more infectious than excised linear DNA inserts. Progeny virus, derived from plants inoculated with cloned DNAs, differed in their ability to infect sugarbeet, L., and the severity of symptoms produced in and other experimental hosts.

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/content/journal/jgv/10.1099/0022-1317-71-10-2211
1990-10-01
2024-12-08
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References

  1. Bennett C. W. 1971; The curly top disease of sugarbeet and other plants. Monographs, the American Phytopathological Society 7:26–28
    [Google Scholar]
  2. Briddon R. W., Watts J., Markham P. G., Stanley J. 1989; The coat protein of beet curly top virus is essential for infectivity. Virology 172:628–633
    [Google Scholar]
  3. Duffus J. E., Skoyen I. O. 1977; Relationship of age of plants and resistance to a severe isolate of the beet curly top virus. Phyto-pathology 67:151–154
    [Google Scholar]
  4. Duffus J. E., Milbrath G. M., Perry R. 1982; Unique type of curly top virus and its relationship with horseradish brittle root. Plant Disease 66:650–652
    [Google Scholar]
  5. Esau K., Hoefert L. L. 1973; Particles and associated inclusions in sugarbeet infected with the curly top virus. Virology 56:454–464
    [Google Scholar]
  6. Gilbertson R. L., Faria J. C., Morales F., Leong S. A., Maxwell D. P., Ahlquist P. G. 1988; Molecular characterization of geminiviruses causing bean golden mosaic. Phytopathology 78:1568
    [Google Scholar]
  7. Grimsley N., Hohn B., Hohn T., Walden R. 1986; “Agroinfection”, an alternative route for viral infection of plants by using the Ti plasmid. Proceedings of the National Academy of Sciences U.S.A: 833282–3286
    [Google Scholar]
  8. Hayes R. J., Brough C. L., Prince V. E., Coutts R. H. A., Buck K. W. 1988; Infection of Nicotiana benthamiana with uncut cloned tandem dimers of tomato golden mosaic virus DNA. Journal of General Virology 69:209–218
    [Google Scholar]
  9. Larsen R. C., Duffus J. E. 1984; A simplified procedure for purification of curly top virus and the isolation of its monomer and dimer particles. Phytopathology 74:114–118
    [Google Scholar]
  10. Lazarowitz S. G. 1987; The molecular characterization of geminiviruses. Plant Molecular Biology Reporter 4:177–192
    [Google Scholar]
  11. Magyarosy A. C., Duffus J. E. 1977; The occurrence of highly virulent strains of the beet curly top virus in California. Plant Disease Reporter 61:248–250
    [Google Scholar]
  12. Maniatis R., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  13. Morinaga T., Ikegami M., Arai T., Yazaki K., Miura K. 1988; Infectivity of cloned tandem dimer DNAs of bean golden mosaic virus. Journal of General Virology 69:879–902
    [Google Scholar]
  14. Severin H. H. P. 1924; Curly leaf transmission experiments. Phytopathology 14:80–93
    [Google Scholar]
  15. Stanley J., Markham P. G., Callis R. J., Pinner M. S. 1986; The nucleotide sequence of an infectious clone of the geminivirus beet curly top virus. EMBO Journal 5:1761–1767
    [Google Scholar]
  16. Stenger D. C., Duffus J. E., Villalon B. 1990; Biological and genomic properties of a geminivirus isolated from pepper. Phytopathology 80 in press
    [Google Scholar]
  17. Thornley W. R., Mumford D. L. 1979; Intracellular location of beet curly top virus antigen as revealed by fluorescent antibody staining. Phytopathology 69:738–740
    [Google Scholar]
  18. Vieira J., Messing J. 1982; The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
    [Google Scholar]
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