1887

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Volume 71, Issue 1

B Cell Epitope Mapping of Human Immunodeficiency Virus Envelope Glycoproteins with Long (19- to 36-residue) Synthetic Peptides Free

Abstract

Envelope glycoproteins, gpl20 and gp41, of the human immunodeficiency virus type 1 (HIV-1) elicit immune responses, including virus-neutralizing antibodies, which are expected to play a role in the defence against HIV-1 infection. Subregions of the gpl20/gp41 sequence have immunosuppressive effects or may be implicated in autoimmune responses. Some of the immunodominant epitopes of gpl20/gp41 do not contribute to protective immunity and act as immunological decoys. These circumstances emphasize the need to select from gpl20/gp41 regions inducing protective responses. Towards this goal, 30 peptides covering approximately 87 % of the HIV-1 strain BH10 gpl20/gp41 sequence were synthesized. Antibodies in rabbit and human anti-HIV-1 sera recognized 28 and nine of the peptides, respectively, indicating that most of the gpl20/gp41 sequence is immunogenic and secondly, that the antibody response to HIV-1 is restricted in infected humans. Most of the peptides, without conjugation to carriers, elicited high levels of anti-peptide (endpoints 1: > 10) and anti-gpl20/gp41 (endpoints 1: ≥ 10) antibodies. The highest levels of virus-neutralizing antibodies were elicited by peptide 306 to 338 from a hypervariable loop of gpl20. Additional peptides from the full-length hypervariable loop (303 to 338) of HIV-1 BH10 and from 20 additional HIV-1 isolates were recognized differentially by human anti-HIV, suggesting that success of passive immunization may depend on a match between administered antibodies and the challenging HIV-1 strain, and also that active immunization with selected peptides from a hypervariable region of distinct HIV-1 isolates should be explored further as a method for prophylaxis against infection.

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© Society for General Microbiology 1990
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1990-01-01
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References

  1. Alexander S., Elder J. H. 1984; Carbohydrate dramatically influences immune reactivity of antisera to viral glycoprotein antigens. Science 226:1328–1330
     [Google Scholar]
  2. Anand R., Thayer R., Srinivasan A., Nayyar S., Gardner M., Luciw P., Dandekar S. 1989; Biological and molecular characterization of human immunodeficiency virus (HIV-1BR) from the brain of a patient with progressive dementia. Virology 168:79–89
     [Google Scholar]
  3. Arthur L. O., Pyle S. W., Nara P. L., Bess J. W.Jr Gonda M. A., Kelliher J. C., Gilden R. V., Robey W. G., Bolognesi D. P., Gallo R. C., Fischinger P. J. 1987; Serological responses in chimpanzees inoculated with human immunodeficiency virus glycoprotein (gpI20) subunit vaccine. Proceedings of the National Academy of Sciences U.S.A.: 848583–8587
     [Google Scholar]
  4. Berman P. W., Groopman J. E., Gregory T., Clapham P. R., Weiss R. A., Ferriani R., Riddle L., Shimasaki C., Lucas C., Lasky L. A., Eichberg J. W. 1988; Human immunodeficiency virus type 1 challenge of chimpanzees immunized with recombinant envelope glycoprotein gpl20. Proceedings of the National Academy of Sciences U.S.A.: 855200–5204
     [Google Scholar]
  5. Berzofsky J. A., Bensussan A., Cease K. B., Bourge J. F., Cheynier R., Lurhuma Z., Salaun J.-J., Gallo R. C., Shearer G. M., Zagury D. 1988; Antigenic peptides recognized by T lymphocytes from AIDS viral envelope-immune humans. Nature; London: 334706–708
     [Google Scholar]
  6. Blumberg R. S., Paradis T., Hartshorn K. L., Vogt M., Ho D. D., Hirsch M. S., Leban J., Sato V. L., Schooley R. T. 1987; Antibody-dependent cell-mediated cytotoxicity against cells infected with the human immunodeficiency virus. Journal of Infectious Diseases 156:878–884
     [Google Scholar]
  7. Brenneman D. E., Westbrook G. L., Fitzgerald S. P., Ennist D. L., Elkins K. L., Ruff M. R., Pert C. B. 1988; Neuronal cell killing by the envelope protein of HIV and its prevention by vasoactive intestinal peptide. Nature; London: 335635–642
     [Google Scholar]
  8. Cauda R., Tumbarello M., Ortona L., Kanda P., Kennedy R. C. 1988; Inhibition of normal human natural killer cell activity by human immunodeficiency virus synthetic transmembrane peptides. Cellular Immunology 115:57–65
     [Google Scholar]
  9. Cease K. B., Margalit H., Cornette J. L., Putney S. D., Robey W. G., Ouyang C., Streicher H. Z., Fischinger P. J., Gallo R. C., Delisi C., Berzofsky J. A. 1987; Helper T-cell antigenic site identification in the acquired immunodeficiency syndrome virus gpl20 envelope protein and induction of immunity in mice to the native protein using a 16-residue synthetic peptide. Proceedings of the National Academy of Sciences U.S.A.: 844249–4253
     [Google Scholar]
  10. Certa U., Bannwarth W., Stuber D., Gentz R., Lanzer M., Legrice S., Guillot F., Wendler I., Hunsmann G., Bujard H., Mous J. 1986; Subregions of a conserved part of the HIV gp41 transmembrane protein are differentially recognized by antibodies of infected individuals. EMBO Journal 5:3051–3056
     [Google Scholar]
  11. Chanh T. C., Dreesman G. R., Kanda P., Linette G. P., Sparrow J. T., Ho D. D., Kennedy R. C. 1986; Induction of anti-HIV neutralizing antibodies by synthetic peptides. EMBO Journal 5:3065–3071
     [Google Scholar]
  12. Chanh T. C., Kennedy R. C., Kanda P. 1988; Synthetic peptides homologous to HIV transmembrane glycoprotein suppress normal human lymphocyte blastogenic response. Cellular Immunology 3:77–86
     [Google Scholar]
  13. Cheng-Mayer C., Seto D., Tateno M., Levy J. A. 1988a; Biologic features of HIV-1 that correlate with virulence in the host. Science 240:80–82
     [Google Scholar]
  14. Cheng-Mayer C., Homsy J., Evans L. A., Levy J. A. 1988b; Identification of human immunodeficiency virus subtypes with distinct patterns of sensitivity to serum neutralization. Proceedings of the National Academy of Sciences U.S.A.: 852815–2819
     [Google Scholar]
  15. Dalgleish A. G., Chanh T. C., Kennedy R. C., Kanda P., Clapham P. R., Weiss R. A. 1988; Neutralization of diverse HIV-1 strains by monoclonal antibodies raised against a gp41 synthetic peptide. Virology 165:209–215
     [Google Scholar]
  16. Dyrberg T., Oldstone M. B. A. 1986; Peptides as antigens: importance of orientation. Journal of Experimental Medicine 164:1344–1349
     [Google Scholar]
  17. Felber B. K., Pavlakis G. N. 1988; A quantitative bioassay for HIV-1 based on trans-activation. Science 239:184–187
     [Google Scholar]
  18. Fisher A. G., Ensoli B., Looney D., Rose A., Gallo R. C., Saag M. S., Shaw G. M., Hahn B. H., Wong-Staal F. 1988; Biologically diverse molecular variants within a single HIV-1 isolate. Nature; London: 334444–447
     [Google Scholar]
  19. Getzoff E. D., Tainer J. A., Lerner R. A., Geysen H. M. 1988; The chemistry and mechanism of antibody binding to protein antigens. Advances in Immunology 43:1–98
     [Google Scholar]
  20. Geyer H., Holschbach C., Hunsmann G., Schneider J. 1988; Carbohydrates of human immunodeficiency virus. Journal of Biological Chemistry 263:11760–11767
     [Google Scholar]
  21. Gnann J. W.Jr Nelson J. A., Oldstone M. B. A. 1987a; Fine mapping of an immunodominant domain in the transmembrane glycoprotein of human immunodeficiency virus. Journal of Virology 61:2639–2641
     [Google Scholar]
  22. Gnann J. W.Jr Schwimmbeck P. L., Nelson J. A., Truax A. B., Oldstone M. B. A. 1987b; Diagnosis of AIDS by using a 12- amino acid peptide representing an immunodominant epitope of the human immunodeficiency virus. Journal of Infectious Diseases 156:261–267
     [Google Scholar]
  23. Golding H., Robey F. A., Gates F. T.III Linder W., Beining P. R., Hoffman T., Golding B. 1988; Identification of homologous regions in human immunodeficiency virus I gp41 and human MHC class II β1 domain. Journal of Experimental Medicine 167:914–923
     [Google Scholar]
  24. Goudsmit J. 1988; Immunodominant B-cell epitopes of the HIV-1 envelope recognized by infected and immunized hosts. AIDS 2:S41–S45
     [Google Scholar]
  25. Goudsmit J., Debouck C., Meloen R. H., Smit L., Barker M., Asher D. M., Wolff A. V., Gibbs C. J.Jr Gajdusek D. C. 1988; Human immunodeficiency virus type 1 neutralization epitope with conserved architecture elicits early type-specific antibodies in experimentally infected chimpanzees. Proceedings of the National Academy of Sciences U.S.A.: 854478–4482
     [Google Scholar]
  26. Hahn B. H., Shaw G. M., Taylor M. E., Redfield R. R., Markham P. D., Salahuddin S. Z., Wong-Staal F., Gallo R. C., Parks E. S., Parks W. P. 1986; Genetic variation in HLTV-III/LAV over time in patients with AIDS or at risk for AIDS. Science 232:1548–1553
     [Google Scholar]
  27. Harada S., Koyanagi Y., Yamamoto N. 1985; Infection of HTLV-III/LAV in HTLV-I-carrying cells MT-2 and MT-4 and application in a plaque assay. Science 229:563–566
     [Google Scholar]
  28. Ho D. D., Sarngadharan M. G., Hirsch M. S., Schooley R. T., Rota T. R., Kennedy R. C., Chanh T. C., Sato V. L. 1987; Human immunodeficiency virus neutralizing antibodies recognize several conserved domains on the envelope glycoproteins. Journal of Virology 61:2024–2028
     [Google Scholar]
  29. Ho D. D., Kaplan J. C., Rackauskas I. E., Gurney M. E. 1988; Second conserved domain of gpl20 is important for HIV infectivity and antibody neutralization. Science 239:1021–1023
     [Google Scholar]
  30. Hu S.-L., Fultz P. N., Mcclure H. M., Eichberg J. W., Thomas E. K., Zarling J., Singhal M. C., Kosowski S. G., Swensor R. B., Anderson D. C., Todaro G. 1987; Effect of immunization with a vaccinia-HIV envrecombinant on HIV infection of chimpanzees. Nature; London: 328721–723
     [Google Scholar]
  31. Kent S., Clark-Lewis I. 1985; Modem methods for the chemical synthesis of biologically active peptides. In Synthetic Peptides in Biology and Medicine pp 29–57 Alitalo K., Partanen P., Vaheri A. Edited by Amsterdam: Elsevier;
     [Google Scholar]
  32. Klasse P. J., Pipkorn R., Blomberg J. 1988; Presence of antibodies to a putatively immunosuppressive part of human immunodeficiency virus (HIV) envelope glycoprotein gp41 is strongly associated with health among HIV-positive subjects. Proceedings of the National Academy of Sciences U.S.A.: 855225–5229
     [Google Scholar]
  33. Koenig S., Earl P., Powell D., Pantaleo G., Merli S., Moss B., Fauci A. S. 1988; Group-specific, major histocompatibility complex class I-restricted cytotoxic responses to human immunodeficiency vims 1 (HIV-1) envelope proteins by cloned peripheral blood T cells from an HIV-l-infected individual. Proceedings of the National Academy of Sciences U.S.A.: 858638–8642
     [Google Scholar]
  34. Koup R. A., Sullivan J. L., Levine P. H., Brewster F., Mahr A., Mazzara G., Mckenzie S., Panicali D. 1989; Antigenic specificity of antibody-dependent cell-mediated cytotoxicity directed against human immunodeficiency vims in antibody-positive sera. Journal of Virology 63:584–590
     [Google Scholar]
  35. Krohn K., Robey W. G., Putney S., Arthur L., Nara P., Fischinger P., Gallo R. C., Wong-Staal F., Ranki A. 1987; Specific cellular immune response and neutralizing antibodies in goats immunized with native or recombinant envelope proteins derived from human T-lymphotropic virus type IIIB and in human immunodeficiency virus-infected men. Proceedings of the National Academy of Sciences U.S.A.: 844994–4998
     [Google Scholar]
  36. Lasky L. A., Groopman J. E., Fennie C. W., Benz P. M., Capon D. J., Dowbenko D. J., Nakamura G. R., Nunes W. M., Renz M. E., Berman P. W. 1986; Neutralization of the AIDS retrovirus by antibodies to a recombinant envelope glycoprotein. Science 233:209–212
     [Google Scholar]
  37. Linsley P. S., Ledbetter J. A., Kinney-Thomas E., Hu S.-L. 1988; Effects of anti-gp 120 monoclonal antibodies on CD4 receptor binding by the env protein of human immunodeficiency virus type 1. Journal of Virology 62:3695–3702
     [Google Scholar]
  38. Lodish H. F., Kong N., Snider M., Strous G.J.A.M. 1983; Hepatoma secretory proteins migrate from endoplasmic reticulum to Golgi at characteristic rates. Nature; London: 30480–83
     [Google Scholar]
  39. Looney D. J., Fisher A. G., Putney S. D., Rusche J. R., Redfield R. R., Burke D. S., Gallo R. C., Wong-Staal F. 1988; Type- restricted neutralization of molecular clones of human immunodeficiency virus. Science 241:357–359
     [Google Scholar]
  40. Lyerly H. K., Reed D. L., Matthews T. J., Langlois A. J., Ahearne P. A., Petteway S. R.Jr Weinhold K. J. 1987a; Anti-GP 120 antibodies from HIV seropositive individuals mediate broadly reactive anti-HIV ADCC. AIDS Research and Human Retroviruses 3:409–422
     [Google Scholar]
  41. Lyerly H. K., Matthews T. J., Langlois A. J., Bolognesi D. P., Weinhold K. J. 1987b; Human T-cell lymphotropic virus IIIBglycoprotein (gpl20) bound to CD4 determinants on normal lymphocytes and expressed by infected cells serves as target for immune attack. Proceedings of the National Academy of Sciences U.S A.: 844601–4605
     [Google Scholar]
  42. Maddon P. J., Dalgleish A. G., Mcdougall J. S., Clapham P. R., Weiss R. A., Axel R. 1986; The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brain. Cell 47:333–348
     [Google Scholar]
  43. Matsushita S., Robert-Guroff M., Rusche J., Koito A., Hattori T., Hoshino H., Javaherian K., Takatsuki K., Putney S. D. 1988; Characterization of a human immunodeficiency virus neutralizing monoclonal antibody and mapping of the neutralizing epitope. Journal of Virology 62:2107–2114
     [Google Scholar]
  44. Matthews T. J., Langlois A. J., Robey W. G., Chang N. T., Gallo R. C., Fischinger P. J., Bolognesi D. P. 1986; Restricted neutralization of divergent human T-lymphotropic virus type III isolates by antibodies to the major envelope glycoprotein. Proceedings of the National Academy of Sciences U.S.A.: 839709–9713
     [Google Scholar]
  45. Modrow S., Hahn B. H., Shaw G. M., Gallo R. C., Wong-Staal F., Wolf H. 1987; Computer-assisted analysis of envelope protein sequences of seven human immunodeficiency virus isolates: prediction of antigenic epitopes in conserved and variable regions. Journal of Virology 2:570–578
     [Google Scholar]
  46. Modrow S., Hoflacher B., Mellert W., Erfle V., Wahren B., Wolf H. 1989; Use of synthetic oligopeptides in identification and characterization of immunological functions in the amino acid sequence of the envelope protein of HIV-1. Journal of Acquired Immune Deficiency Syndromes 2:21–27
     [Google Scholar]
  47. Myers G., Rabson A. B., Josephs S. F., Smith T. F., Wong-Staal F. 1988 Human Retroviruses and AIDS 1988: a Compilation and Analysis of Nucleic Acid and Amino Acid Sequences pp II-68–II-91 Los Alamos: Los Alamos National Laboratory;
     [Google Scholar]
  48. Nair M. P. N., Pottathil R., Heimer E. P., Schwartz S. A. 1988; Immunoregulatory activities of human immunodeficiency virus (HIV) proteins: effect of HIV recombinant and synthetic peptides on immunoglobulin synthesis and proliferative responses by normal lymphocytes. Proceedings of the National Academy of Sciences U.S.A: 856498–6502
     [Google Scholar]
  49. Nara P. L., Robey W. G., Arthur L. O., Asher D. M., Wolff A. V., Gibbs C. J.Jr Gajdusek D. C., Fischinger P. J. 1987a; Persistent infection of chimpanzees with human immunodeficiency virus: serological responses and properties of reisolated viruses. Journal of Virology 61:3173–3180
     [Google Scholar]
  50. Nara P. L., Robey W. G., Gonda M. A., Carter S. G., Fischinger P. J. 1987b; Absence of cytotoxic antibody to human immunodeficiency virus-infected cells in humans and its induction in animals after infection or immunization with purified envelope glycoprotein gpl20. Proceedings of the National Academy of Sciences U.S.A.: 843797–3801
     [Google Scholar]
  51. Nara P. L., Robey W. G., Pyle S. W., Hatch W. C., Dunlop N. M., Bess J. W.Jr Kelliher J. C, Fischinger P. J. 1988; Purified envelope glycoproteins from human immunodeficiency virus type 1 variants induce individual, type-specific neutralizing antibodies. Journal of Virology 62:2622–2628
     [Google Scholar]
  52. Neurath A. R., Kent S. B. H. 1988; The pre-S region of hepadnavirus envelope proteins. Advances in Virus Research 34:6514
     [Google Scholar]
  53. Neurath A. R., Strick N. 1989; Advances in understanding structure-function relationships in hepatitis B virus (HBV) envelope (env) proteins. In Immunology of Proteins and Peptides237–250 Atassi Z. Edited by New York: Plenum Press;
     [Google Scholar]
  54. Neurath A. R., Kent S. B. H., Strick N., Parker K., Courouce A.-M., Riotot M. M., Petit M. A., Budkowska A., Girard M., Pillot J. 1987; Antibodies to synthetic peptides from the pre-Sl and pre-S2 regions of one subtype of the hepatitis B virus (HBV) envelope protein recognize all HBV subtypes. Molecular Immunology 24:975–980
     [Google Scholar]
  55. Neurath A. R., Seto B., Strick N. 1989a; Antibodies to synthetic peptides from the preSl region of the hepatitis B virus (HBV) envelope (env) protein are virus-neutralizing and protective. Vaccine 7:234–236
     [Google Scholar]
  56. Neurath A. R., Strick N., Girard M. 1989b; Hepatitis B virus surface antigen (HBsAg) as carrier for synthetic peptides having an attached hydrophobic tail. Molecular Immunology 26:53–62
     [Google Scholar]
  57. Neurath A. R., Strick N., Kent S. B. H., Parker K., Kim C. S., Girard M., Ralph H. E., Valinsky J. 1989c; Biological role of pre-S sequences of the hepatitis B virus (HBV) envelope protein. In Synthetic Peptides: Approaches to Biological Problems pp 143–158 Kaiser T. Edited by New York: Alan R. Liss;
     [Google Scholar]
  58. Ojo-Amaize E. A., Nishanian P., Keith D. E.Jr Houghton R. L., Heitjan D. F., Fahey J. L., Giorgi J. V. 1987; Antibodies to human immunodeficiency virus in human sera induce cell-mediated lysis of human immunodeficiency virus-infected cells. Journal of Immunology 139:2458–2463
     [Google Scholar]
  59. Palker T. J., Matthews T. J., Clark M. E., Cianciolo G. J., Randall R. R., Langlois A. J., White G. C., Safai B., Snyderman R., Bolognesi D. P., Haynes B. F. 1987; A conserved region at the COOH terminus of human immunodeficiency virus gpl20 envelope protein contains an immunodominant epitope. Proceedings of the National Academy of Sciences U.S.A.: 842479–2483
     [Google Scholar]
  60. Palker T. J., Clark M. E., Langlois A. J., Matthews T. J., Weinhold K. J., Randall R. R., Bolognesi D. P., Haynes B. F. 1988; Type-specific neutralization of the human immunodeficiency virus with antibodies to enc-encoded synthetic peptides. Proceedings of the National Academy of Sciences U.S.A.: 851932–1936
     [Google Scholar]
  61. Pinter A., Honnen W. J., Tilley S. A., Bona C., Zaghouani H., Gorny M. K., Zola-Pazner S. 1989; Oligomeric structure of gp41, the transmembrane protein of human immunodeficiency virus type 1. Journal of Virology 63:2674–2679
     [Google Scholar]
  62. Plaue S., Heissler D. 1987; A new preparation of 4-(boc-amino- acyloxymethyl)phenylacetic acids for solid-phase peptide synthesis. Tetrahedron Letters 28:1401–1404
     [Google Scholar]
  63. Prince A. M., Horowitz B., Baker L., Shulman R. W., Ralph H., Valinsky J., Cundell A., Brotman B., Boehle W., Rey F., Piet M., Reesink H., Lelie N. C., Tersmette M., Miedema F., Barbosa L., Nemo G., Nastala C. L., Allan J. S., Lee D. R., Eichberg J. W. 1988; Failure of a human immunodeficiency virus (HIV) immune globulin to protect chimpanzees against experimental challenge with HIV. Proceedings of the National Academy of Sciences U.S.A.: 856944–6948
     [Google Scholar]
  64. Putney S. D., Matthews T. J., Robey W. G., Lynn D. L., Robert-Guroff M., Mueller W. T., Langlois A. J., Ghrayeb J., Petteway S. R.Jr Weinhold K. J., Fischinger P. J., Wong-Staal F., Gallo R. C., Bolognesi D. P. 1986; HTLV- III/LAV-neutralizing antibodies of an E. coli-produced fragment of the virus envelope. Science 234:1392–1395
     [Google Scholar]
  65. Putney S., Rusche J., Matthews T. J., Krohn K., Carson H., Lynn D., Jackson J., Robey W. G., Ranki A., Robert-Guroff M., Gallo R. C., Bolognesi D. P. 1988; HIV neutralizing antibodies elicited by recombinant envelope proteins. In Human Retroviruses, Cancer and AIDS: Approaches to Prevention and Therapy pp 149–159 Robinson W., Koike K., Hill H. Edited by New York: Alan R. Liss;
     [Google Scholar]
  66. Ratner L., Haseltine W., Patarca R., Livak K. J., Starcich B., Josephs S. F., Doran E. R., Rafalski J. A., Whitehorn E. A., Baumeister K., Ivanoff L., Petteway S. R.Jr Pearson M. L., Lautenberger J. A., Papas T. S., Ghrayeb J., Chang N. T., Gallo R. C., Wong-Staal F. 1985; Complete nucleotide sequence of the AIDS virus, HTLV-III. Nature; London: 313277–284
     [Google Scholar]
  67. Reiher W. E.III Blalock J. E., Brunck T. K. 1986; Sequence homology between acquired immunodeficiency syndrome virus envelope protein and interleukin 2. Proceedings of the National Academy of Sciences U.S.A.: 839188–9192
     [Google Scholar]
  68. Reitz M. S.Jr Wilson C., Naugle C., Gallo R. C., Robert-Guroff M. 1988; Generation of a neutralization-resistant variant of HIV-1 is due to selection for a point mutation in the envelope gene. Cell 54:57–63
     [Google Scholar]
  69. Ritchie D. G., Nickerson J. M., Fuller G. M. 1983; Two simple programs for the analysis of data from enzyme-linked immuno-sorbent assays (ELISA) on a programmable desk-top calculator. Methods in Enzymology 92:577–588
     [Google Scholar]
  70. Robert-Guroff M., Reitz M. S.Jr Robey W. G., Gallo R. C. 1986; In vitro generation of an HTLV-III variant by neutralizing antibody. Journal of Immunology 137:3306–3309
     [Google Scholar]
  71. Robey W. G., Arthur L. O., Matthews T. J., Langlois A., Copeland T. D., Lerche N. W., Oroszlan S., Bolognesi D. P., Gilden R. V., Fischinger P. J. 1986; Prospect for prevention of human immunodeficiency virus infection: purified 120-kDa envelope glycoprotein induces neutralizing antibody. Proceedings of the National Academy of Sciences U.S.A.: 837023–7027
     [Google Scholar]
  72. Rusche J. R., Lynn D. L., Robert-Guroff M., Langlois A. J., Lyerly H. K., Carson H., Krohn K., Ranki A., Gallo R. C., Bolognesi D. P., Putney S. D., Matthews T. J. 1987; Humoral immune response to the entire human immunodeficiency virus envelope glycoprotein made in insect cells. Proceedings of the National Academy of Sciences U.S.A.: 846924–6928
     [Google Scholar]
  73. Rusche J. R., Javaherian K., Mcdanal C., Petro J., Lynn D. L., Grimaila R., Langlois A., Gallo R. C., Arthur L. O., Fischinger P. J., Bolognesi D. P., Putney S. D., Matthews T. J. 1988; Antibodies that inhibit fusion of human immunodeficiency virus-infected cells bind a 24-amino acid sequence of the viral envelope, gpl20. Proceedings of the National Academy of Sciences U.S.A.: 853198–3202
     [Google Scholar]
  74. Saag M. S., Hahn B. H., Gibbons J., Li Y., Parks E. S., Parks W. P., Shaw G. M. 1988; Extensive variation of human immunodeficiency virus type-1 in vivo. Nature; London: 334440–444
     [Google Scholar]
  75. Schaaper W. M. M., Lankhof H., Puijk W. C., Meloen R. H. 1989; Manipulation of anti-peptide immune response by varying the coupling of the peptide with the carrier protein. Molecular Immunology 26:81–85
     [Google Scholar]
  76. Shepp D. H., Chakrabarti S., Moss B., Quinnan G. V.Jr 1988; Antibody-dependent cellular cytotoxicity specific for the envelope antigens of human immunodeficiency virus. Journal of Infectious Diseases 157:1260–1264
     [Google Scholar]
  77. Siliciano R. F., Lawton T., Knall C., Karr R. W., Berman P., Gregory T., Reinherz E. L. 1988; Analysis of host-virus interactions in AIDS with anti-gpl20 T cell clones: effect of HIV sequence variation and a mechanism for CD4+ cell depletion. Cell 54:561–575
     [Google Scholar]
  78. Takahashi H., Cohen J., Hosmalin A., Cease K. B., Houghten R., Cornette J. L., Delisi C., Moss B., Germain R. N., Berzofsky J. A. 1988; An immunodominant epitope of the human immunodeficiency virus envelope glycoprotein gpl60 recognized by class I major histocompatibility complex molecule-restricted murine cytotoxic T lymphocytes. Proceedings of the National Academy of Sciences U.S.A.: 853105–3109
     [Google Scholar]
  79. Thomas E. K., Weber J. N., Mcclure J., Clapham P. R., Singhal M. C., Shriver M. K., Weiss R. A. 1988; Neutralizing monoclonal antibodies to the AIDS virus. AIDS 2:25–29
     [Google Scholar]
  80. Tyler D. S., Nastala C. L., Stanley S. D., Matthews T. J., Lyerly H. K., Bolognesi D. P., Weinhold K. J. 1989; GP120 specific cellular cytotoxicity in HIV-1 seropositive individuals: evidence for circulating CD16+ effector cells armed in vivo with cytophilic antibody. Journal of Immunology 142:1177–1182
     [Google Scholar]
  81. Van Regenmortel M. H. V., Muller S., Quesniaux V. F., Altschuh D., Briand J. P. 1988a; Operational aspects of epitope identification: structural features of proteins recognized by antibodies. In Vaccines: New Concepts and Developments pp Köhler H., Laverde P. T. Edited by London: Longman;
     [Google Scholar]
  82. Van Regenmortel M. H. V., Briand J. P., Muller S., Plaue S. 1988b; Synthetic polypeptides as antigens. In Laboratory Techniques in Biochemistry and Molecular Biology 19 pp 1–227 Burdon R. H., Van Knippenberg P. H. Edited by Amsterdam: Elsevier;
     [Google Scholar]
  83. Walker B. D., Chakrabarti S., Moss B., Paradis T. J., Flynn T., Durno A. G., Blumberg R. S., Kaplan J. C., Hirsch M. S., Schooley R. T. 1987; HIV-specific cytotoxic T lymphocytes in seropositive individuals. Nature; London: 328345–348
     [Google Scholar]
  84. Wang J. J. G., Steel S., Wisniewolski R., Wang C. Y. 1986; Detection of antibodies to human T-lymphotropic virus type III by using a synthetic peptide of 21 amino acid residues corresponding to a highly antigenic segment of gp41 envelope protein. Proceedings of the National Academy of Sciences U.S.A.: 83:6159–6163
     [Google Scholar]
  85. Weiss R. A., Clapham P. R., Weber J. N., Dalgleish A. G., Lasky L. A., Berman P. W. 1986; Variable and conserved neutralization antigens of human immunodeficiency virus. Nature; London: 324572–575
     [Google Scholar]
  86. Zagury D., Bernard J., Cheynier R., Desportes I., Leonard R., Fouchard M., Reveil B., Ittele D., Lurhuma Z., Mbayo K., Wane J., Salaun J.-J., Goussard B., Dechazal L., Burny A., Nara P., Gallo R. C. 1988; A group-specific anamnestic immune reaction against HIV-1 induced by a candidate vaccine against AIDS. Nature; London: 332728–731
     [Google Scholar]
  87. Zweig S. D., Showalter S. V., Bladen S. V., Gilden R. V., Arthur L. O., Robey W. G., Nara P. L., Fischinger P. G. 1988; Partial purification of native HIV transmembrane protein gp41: generation of polyclonal and monoclonal antibodies. AIDS Research and Human Retroviruses 4:51–62
     [Google Scholar]
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B Cell Epitope Mapping of Human Immunodeficiency Virus Envelope Glycoproteins with Long (19- to 36-residue) Synthetic Peptides
J. Gen. Virol. 71, 85 (1990); https://doi.org/10.1099/0022-1317-71-1-85
/content/journal/jgv/10.1099/0022-1317-71-1-85
/content/journal/jgv/10.1099/0022-1317-71-1-85
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