1887

Abstract

The mechanism of neutralization of a type A influenza virus by polyclonal IgM was similar for both tracheal epithelial and BHK cells. Maximum neutralization was only 90% and most (70%) of the virus failed to attach to inoculated cells. The remainder attached to -acetylneuraminic acid receptors but was not internalized. IgM aggregated virus, but only at an IgM:virus ratio below the level required for neutralization. Failure to detect any loss of infectivity associated with aggregation suggested that aggregates were unstable. Monoclonal polymeric IgA neutralized virus more efficiently on BHK cells (99·9%) than the equivalent amounts of IgM (90%). Otherwise the mechanisms of IgA and IgM neutralization were similar, except that IgA-induced aggregation was coincident with loss of infectivity and may thus have contributed to it. However, IgA-neutralized virus attached to tracheal epithelial cells more efficiently than infectious virus, initially using a neuraminidase-sensitive receptor, but then becoming neuraminidase-resistant. Whether the latter IgA-virus complexes were internalized or attached to a neuraminidase-resistant receptor is not known. This use of differentiated murine cells with murine IgA gave neutralization data that differed qualitatively from those obtained with the same antibody and undifferentiated hamster cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-1-69
1990-01-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/1/JV0710010069.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-1-69&mimeType=html&fmt=ahah

References

  1. Dimmock N. J. 1984; Mechanisms of neutralization of animal viruses. Journal of General Virology 65:1015–1022
    [Google Scholar]
  2. Dimmock N. J. 1987; Multiple mechanisms of neutralization of animal viruses. Trends in Biochemical Sciences 12:70–75
    [Google Scholar]
  3. Dimmock N. J., Carver A. S., Webster R. G. 1980; Categorization of nucleoproteins and matrix proteins from type A influenza viruses by peptide mapping. Virology 103:350–356
    [Google Scholar]
  4. Dimmock N. J., Taylor H. P., Carver A. S. 1984; Interaction of neutralized influenza virus with avian and mammalian cells. In Segmented Negative Strand Viruses pp. 355–359 Compans R. W., Bishop D. H. L. Edited by New York: Academic Press;
    [Google Scholar]
  5. Eisenlohr L. C., Gerhard W., Hackett C. J. 1987; Role of receptor-binding activity of the viral hemagglutinin molecule in the presentation of influenza virus antigens to helper T cells. Journal of Virology 61:1375–1383
    [Google Scholar]
  6. Hoorn B. 1966; Organ cultures of ciliated epithelium for the study of respiratory viruses. Acta pathologica et microbiologica scandanavica, supplemenlum 183 66:1–37
    [Google Scholar]
  7. Matlin K. S., Reggio H., Helenius A., Simon K. 1981; Infectious entry pathway of influenza virus in a canine kidney cell line. Journal of Cell Biology 91:601–613
    [Google Scholar]
  8. Morser M. J., Kennedy S. I. T., Burke D. C. 1973; Virus- specified polypeptides in cells infected with Semliki Forest virus. Journal of General Virology 21:19–29
    [Google Scholar]
  9. Possee R. D., Schild G. C., Dimmock N. J. 1982; Studies on the mechanism of neutralization of influenza virus by antibody: evidence that neutralizing antibody (anti-haemagglutinin) inactivates influenza virus in vivoby inhibiting virion transcriptase activity. Journal of General Virology 58:373–386
    [Google Scholar]
  10. Richman D. D., Hostetler K. Y., Yazaki P. J., Clark S. 1986; Fate of influenza A virion proteins after entry into subcellular fractions of LLC cells and the effect of amantadine. Journal of Virology 151:200–210
    [Google Scholar]
  11. Rigg R. J., Carver A. S., Dimmock N. J. 1989; IgG-neutralized influenza virus undergoes primary, but not secondary uncoating in vivo . Journal of General Virology 70:2097–2109
    [Google Scholar]
  12. Taylor H. P., Dimmock N. J. 1985a; Mechanism of neutralization of influenza virus by secretory IgA is different from that of monomeric IgA or IgG. Journal of Experimental Medicine 161:198–209
    [Google Scholar]
  13. Taylor H. P., Dimmock N. J. 1985b; Mechanisms of neutralization of influenza virus by IgM. Journal of General Virology 66:903–907
    [Google Scholar]
  14. Taylor H. P., Armstrong S. J., Dimmock N. J. 1987; Quantitative relationships between an influenza virus and neutralizing antibody. Virology 159:288–298
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-1-69
Loading
/content/journal/jgv/10.1099/0022-1317-71-1-69
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error