1887

Abstract

The antiviral effects of 6-diazo-5-oxo--norleucine (L-DON) on the replication of human parainfluenza virus type 2 (HPIV-2), mumps and vesicular stomatitis viruses were studied. L-DON suppressed growth of these viruses and, in particular, HPIV-2 in four cell types. L-DON was not toxic to the cells at the active dose and did not significantly inhibit cellular macromolecular synthesis. The L-DON-sensitive step of HPIV-2 replication was considered to be relatively early. The NP, P and M proteins were, although at a low level, clearly detectable in HPIV-2-infected Vero cells treated with L-DON, whereas the HN and F proteins were scarcely detected by either immunostaining or immunoprecipitation, indicating that L-DON mainly decreased the amounts of viral glycoproteins. Furthermore, Northern blot hybridization showed that secondary transcription of virus RNA was also inhibited.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-1-61
1990-01-01
2021-10-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/1/JV0710010061.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-1-61&mimeType=html&fmt=ahah

References

  1. Courteney R. J., Mccombs R. M., Benyesh-Melnick M. 1970; Antigens specified by herpesviruses. I. Effect of arginine deprivation on antigen synthesis. Virology 40:379–386
    [Google Scholar]
  2. Ehrlich J., Coffey G. L., Fisher M. W., Hillegas A. B., Kohberger D. L., Machamer H. E., Rightsel W. A., Roegner F. R. 1956; 6-Diazo-5-oxo-l-norleucine, a new tumor-inhibitory substance. I. Biologic studies. Antibiotics and Chemotherapy 6:487–497
    [Google Scholar]
  3. Goldstein G., Guskey L. E. 1984; Poliovirus and vesicular stomatitis virus replication in the presence of 6-diazo-5-oxo-l-norleucine or 2-deoxy-d-glucose. Journal of Medical Virology 14:159–167
    [Google Scholar]
  4. Hartman S. C., McGrath T. F. 1973; Glutaminase A of Escherichia coli;reactions with the substrate analogue, 6-diazo-5-oxo-norleucine. Journal of Biological Chemistry 248:8506–8510
    [Google Scholar]
  5. Hirayama N., Senda M., Kurata K., Yoshikawa Y. 1985; Requirement of methionine for the replication of canine distemper virus in Vero cells. Journal of General Virology 66:149–157
    [Google Scholar]
  6. Iinuma M., Maeno K., Matumoto T. 1973; Studies on the assembly of Newcastle disease virus, an arginine-dependent step in virus replication. Virology 51:205–215
    [Google Scholar]
  7. Inglis V. B. M. 1968; Requirement of arginine for the replication of herpes virus. Journal of General Virology 3:9–17
    [Google Scholar]
  8. Ito Y., Okazaki H., Sakuma S., Homma M., Ishida N. 1969; Specific requirement of serine for the growth of Newcastle disease virus. Virology 39:277–285
    [Google Scholar]
  9. Ito Y., Kimura Y., Nagata I., Kunii A. 1974; Effects of l-glutamine deprivation on growth of HVJ (Sendai virus) in BHK cells. Journal of Virology 13:557–566
    [Google Scholar]
  10. Ito Y., Tsurudome M., Hishiyama M. 1985; Persistence of mumps virus in mouse L929 cells. Microbiology and Immunology 29:847–857
    [Google Scholar]
  11. Ito Y., Tsurudome M., Hishiyama M. 1987; The polypeptides of human parainfluenza type 2 virus and their synthesis in infected cells. Archives of Virology 95:211–224
    [Google Scholar]
  12. Kawano M., Bando H., Yuasa T., Kondo K., Tsurudome M., Komada H., Nishio M., Ito Y. 1989; Sequence determination of the hemagglutinin-neuraminidase (HN) gene of human parainfluenza type 2 virus and the construction of a phylogenetic tree for HN proteins of all the paramyxoviruses that are infectious to humans. Virology in press
    [Google Scholar]
  13. King G., Bounous C., Holmes E. 1978; Human placental amidophosphoribosyl-transferase: comparison of the kinetics of glutamine and ammonia utilization. Journal of Biological Chemistry 253:3933–3938
    [Google Scholar]
  14. Levine S., Buthala D. A., Hamilton R. D. 1971; Late stage synchronization of respiratory syncytial virus replication. Virology 45:390–400
    [Google Scholar]
  15. Loh P. C., Oie H. K. 1969; Role of lysine in the replication of reovirus. I. Synthesis of complete and empty virions. Journal of Virology 4:890–895
    [Google Scholar]
  16. Novogrodsky A., Tate S. S., Meister A. 1977; Uptake and utilization of l-glutamine by human lymphoid cells; relationship to γ-glutamyl transpeptidase activity. Biochemical and Biophysical Research Communications 78:222–229
    [Google Scholar]
  17. Rouse H. C., Schlesinger R. W. 1967; An arginine-dependent step in the maturation of type 2 adenovirus. Virology 33:513–522
    [Google Scholar]
  18. Spring S. B., Roizman B., Spear P. G. 1969; Selective failure of protein synthesis in herpes-infected cells deprived of arginine. Virology 38:710–712
    [Google Scholar]
  19. Tankersley R. W. 1964; Amino acid requirements of herpes simplex virus in human cells. Journal of Bacteriology 87:609–613
    [Google Scholar]
  20. Tsurudome M., Yamada A., Hishiyama M., Ito Y. 1986; Monoclonal antibodies against the glycoproteins of mumps virus: fusion inhibition by anti-HN monoclonal antibody. Journal of General Virology 67:2259–2265
    [Google Scholar]
  21. Tsurudome M., Nishio M., Komada H., Bando H., Ito Y. 1989; Extensive antigenic diversity among human parainfluenza type 2 virus isolates and immunological relationships among paramyxoviruses revealed by monoclonal antibodies. Virology 171:38–48
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-1-61
Loading
/content/journal/jgv/10.1099/0022-1317-71-1-61
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error