1887

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Volume 71, Issue 1

Localization of Genomic Regions Specific for the Attenuated, Mouse-adapted Poliovirus Type 2 Strain W-2 Free

Abstract

In order to begin to elucidate the genomic basis of the attenuation of mouse-adapted, poliovirus type 2 strain W-2 (PV2/W-2), we have cloned and sequenced the virus and compared it with the virulent, mouse-adapted PV2/Lansing strain. In addition, we have performed computer-generated comparisons of PV2/W-2 to the non-mouse-adapted, attenuated PV2/Sabin strain to determine whether mutational patterns occur that result in attenuation. The PV2/W-2 genome is 7434 nucleotides in length, which is three bases shorter than PV2/Lansing. The 5′ non-coding region of PV2/W-2 is 747 nucleotides in length (compared to 744 in PV2/Lansing) and shares 98·8% identity with PV2/Lansing and 82·3% identity with PV2/Sabin. Overall, the PV2/W-2 polyprotein (2205 amino acids) is two amino acids shorter than that of either PV2/Lansing or PV2/Sabin (2207 amino acids). These contiguous deletions fall within the P3-D region (polymerase). Within these 2205 amino acid residues only 26 differences were observed between PV2/W-2 and PV2/Lansing (98-8% identity), whereas 92 occurred between PV2/W-2 and PV2/Sabin (95·8% identity). The 3′ non-coding region of PV2/W-2 is 72 nucleotides in length and shares 100% identity with PV2/Lansing and 98-6% identity with PV2/Sabin. Amino acid changes in the capsid protein region occurred in neutralization sites 1 and 3, areas previously shown to be important for pathogenicity. The cleavage site between non-structural proteins P2-C/P3- A consisted of a glutamine-serine pair, in contrast to other sequenced polioviruses which have a glutamine- glycine dipeptide.

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© Society for General Microbiology 1990
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1990-01-01
2024-04-16
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Localization of Genomic Regions Specific for the Attenuated, Mouse-adapted Poliovirus Type 2 Strain W-2
J. Gen. Virol. 71, 43 (1990); https://doi.org/10.1099/0022-1317-71-1-43
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