RT Journal Article SR Electronic(1) A1 Smith, A. R. A1 Boursnell, M. E. G. A1 Binns, M. M. A1 Brown, T. D. K. A1 Inglis, S. C.YR 1990 T1 Identification of a New Membrane-associated Polypeptide Specified by the Coronavirus Infectious Bronchitis Virus JF Journal of General Virology, VO 71 IS 1 SP 3 OP 11 DO https://doi.org/10.1099/0022-1317-71-1-3 PB Microbiology Society, SN 1465-2099, AB Nucleotide sequences from the third open reading frame of mRNA D (D3) of infectious bronchitis virus (IBV) were expressed in bacteria as part of a fusion protein with β-galactosidase. Antiserum raised in rabbits against this fusion protein immunoprecipitated from IBV-infected chick kidney or Vero cells a polypeptide of 12·4K, the size expected for a D3- encoded product. The D3 polypeptide is apparently non-glycosylated, and appears to be associated with the membrane fraction of infected cells, as judged by cell fractionation and immunofluorescence., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-71-1-3