%0 Journal Article %A Smith, A. R. %A Boursnell, M. E. G. %A Binns, M. M. %A Brown, T. D. K. %A Inglis, S. C. %T Identification of a New Membrane-associated Polypeptide Specified by the Coronavirus Infectious Bronchitis Virus %D 1990 %J Journal of General Virology, %V 71 %N 1 %P 3-11 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-71-1-3 %I Microbiology Society, %X Nucleotide sequences from the third open reading frame of mRNA D (D3) of infectious bronchitis virus (IBV) were expressed in bacteria as part of a fusion protein with β-galactosidase. Antiserum raised in rabbits against this fusion protein immunoprecipitated from IBV-infected chick kidney or Vero cells a polypeptide of 12·4K, the size expected for a D3- encoded product. The D3 polypeptide is apparently non-glycosylated, and appears to be associated with the membrane fraction of infected cells, as judged by cell fractionation and immunofluorescence. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-71-1-3