1887

Abstract

Two monoclonal antibodies (MAbs) against p27 and one against p17 of simian immunodeficiency virus (SIV) from rhesus macaques were produced and characterized by reacting with disrupted, viral antigens on immunoblots. Human immunodeficiency virus type 1 (HIV-1), HIV-2 and SIV isolates from sooty mangabey, stump-tailed macaque, rhesus macaque and African green monkey (SIV ,SIV, SIV and SIV) were used for comparative analysis. The p27 monoclonal antibodies HE and FA reacted with SIVand SIV, but not with HIV-1, HIV-2,

SIV and SIV. The p17 monoclonal antibodies reacted with SIVand SIV, but not HIV-1, HIV- 2, SIVand SIV. The differential reactivity of these monoclonal antibodies indicated that common conserved antigenic epitopes are shared between SIV and SIV with respect to p27 MAbs and between SIV and SIV with respect to p17. Since these MAbs reacted differently with the SIV isolates, they are useful reagents for comparative pathogenesis studies for differentiating SIV isolates.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-1-247
1990-01-01
2023-02-01
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/1/JV0710010247.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-1-247&mimeType=html&fmt=ahah

References

  1. Benveniste R. E., Morton W. R., Clark G. A., Tsai C. C., Ochs H. D., Ward J. M., Kelhn L., Knott W. B., Hill R. W., Gale M. J., Thouless M. E. 1988; Inoculation of baboons and macaques with simian immunodeficiency virus (Mne), a primate lentivirus closely related to human immunodeficiency virus type-2. Journal of Virology 62:2091–2101
    [Google Scholar]
  2. Clavel F., Guetard D., Brun-Vezinet F., Chamaret S., Rey M., Santos-Ferreira M. O., Laurent A. G., Dauguet C., Katlama C., Rouzioux C., Klatzmann D., Champalimaud J. L., Montagnier L. 1986; Isolation of a new human retrovirus from West African patients with AIDS. Science 233:343–346
    [Google Scholar]
  3. Daniel M. D., Letvin N. L., King N. W., Kannagi M., Sehgal P. K., Kanki P. J., Essex M., Desrosiers R. C. 1985; Isolation of T-cell tropic HTLV-III-like retrovirus from macaques. Science 228:1201–1204
    [Google Scholar]
  4. Daniel M. D., Letvin N. L., Sehgal P. K., Schmidt D. K., Silva D. P., Solomon K. R., Hodi F. S., Ringler D. J., Hunt R. D., King N. W., Desrosiers R. C. 1988a; Prevalence of antibodies to 3 retroviruses in a captive colony of macaque monkeys. International Journal of Cancer 41:601–608
    [Google Scholar]
  5. Daniel M. D., Li Y., Naidu Y. M., Durda P. J., Schmidt D. K., Troup C. D., Silva D. P., Mackey J. J., Kestler H. W. III Sehgal P. K., King N. W., Ohta Y., Hayami M., Desrosiers R. C. 1988b; Simian immunodeficiency virus from African green monkeys. Journal of Virology 62:4123–4128
    [Google Scholar]
  6. Fultz P. N., Mcclure H. M., Anderson D. C., Swenson R. B., Anand R., Srivasan A. 1986; Isolation of a T-lymphotropic retrovirus from naturally infected sooty mangabey monkeys (Cercocebus atys). Proceedings of the National Academy of Sciences, U.S.A 83:5286–5290
    [Google Scholar]
  7. Henderson L. E., Benveniste S. M., Sowder R., Copeland T. D., Schultz A. M., Oroszlan S. 1988; Molecular characterization of gag proteins from simian immunodeficiency virus (SIVMne). Journal of Virology 62:2587–2595
    [Google Scholar]
  8. Kanki P. J., Mclane M. F., King N. W., Letvin N. L., Hunt R. D., Sehgal P. K., Daniel M. D., Desrosiers R. C., Essex M. 1985; Serologic identifications and characterization of macaque T-lymphotropic retrovirus closely related to HTLV-III. Science 228:1199–1201
    [Google Scholar]
  9. Kestler H. W. III Li Y., Naidu Y. N., Butler C. V., Ochs M. F., Jaenel N. W., King N. W., Daniel M. D., Desrosiers R. C. 1988; Comparison of simian immunodeficiency virus isolates. Nature; London: 331619–622
    [Google Scholar]
  10. Li L., Naidu Y. M., Daniel M. D., Desrosiers R. C. 1989; Extensive genetic variability of simian immunodeficiency virus from African green monkeys. Journal of Virology 63:1800–1802
    [Google Scholar]
  11. Lowenstine L. J., Pedersen N. C., Higgins J., Pallis K. C., Uyeda A., Marx P. A., Lerche N. W., Munn R. J., Gardner M. B. 1986; Seroepidemiologic survey of captive Old World primates for antibodies to human and simian retroviruses, and isolation of a lentivirus from sooty mangabeys (Cercocebus atys). International Journal of Cancer 38:563–575
    [Google Scholar]
  12. Lowenstine L. J., Lerche N. W., Marx P. A., Gardner M. B., Pedersen N. C. 1988; An epizootic of simian AIDS caused by SIV in captive macaques in the 1970’s. In Retroviruses of Human AIDS and Related Animal Viruses pp. 174–176 Girard M., Valette L. Edited by Paris: Pasteur Vaccines;
    [Google Scholar]
  13. Minassian A. A., Kalyanaraman V. S., Gallo R. C., Popovic M. 1988; Monoclonal antibodies against human immunodeficiency virus (HIV) type 2 core proteins: cross-reactivity with HIV type 1 and simian immunodeficiency virus. Proceedings of the National Academy of Sciences, U.S.A 85:6939–6943
    [Google Scholar]
  14. Murphey-Corb M., Martin L. N., Rangan S. R. S., Baskin G. B., Gormus B. J., Wolf R. H., Andes W. A., West M., Montelaro R. C. 1986; Isolation of a HTLV-III related retrovirus from macaques with simian AIDS and its possible origin in asymptomatic mangabeys. Nature; London: 321435–437
    [Google Scholar]
  15. Naidu Y. M., Kestler H. W. III Li Y., Butler C. V., Silva D. P., Schmidt D. J., Troup C. D., Sehgal P. K., Sonigo P., Daniel M. D., Desrosiers R. C. 1988; Characterization of infectious molecular clones of SIVMAC and HIV-2: persistent infection of rhesus monkeys with molecularly cloned SIVMAC . Journal of Virology 62:4691–4696
    [Google Scholar]
  16. Niedrig M., Rabanus J.-P., L’age Stehr J., Gelderblom H. R. 1988; Monoclonal antibodies directed against human immunodeficiency virus (HIV) gag proteins with specificity for conserved epitopes in HIV-1, HIV-2 and simian immunodeficiency virus. Journal of General Virology 69:2109–2114
    [Google Scholar]
  17. Ohta Y., Masuda T., Tsujimoto H., Ishikawa K., Kodama T., Morikawa S., Nakai M., Hajo S., Hayami M. 1988; Isolation of simian immunodeficiency virus from African green monkeys and seroepidemiologic survey of the virus in various non-human primates. International Journal of Cancer 41:115–122
    [Google Scholar]
  18. Schneider J., Hunsmann G. 1988; Simian lentiviruses- the SIV group. AIDS 2:1–9
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-1-247
Loading
/content/journal/jgv/10.1099/0022-1317-71-1-247
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error