1887

Abstract

Two monoclonal antibodies (MAbs) against p27 and one against p17 of simian immunodeficiency virus (SIV) from rhesus macaques were produced and characterized by reacting with disrupted, viral antigens on immunoblots. Human immunodeficiency virus type 1 (HIV-1), HIV-2 and SIV isolates from sooty mangabey, stump-tailed macaque, rhesus macaque and African green monkey (SIV, SIV, SIV and SIV) were used for comparative analysis. The p27 monoclonal antibodies HE and FA reacted with SIV and SIV, but not with HIV-1, HIV-2, SIV and SIV. The p17 monoclonal antibodies reacted with SIV and SIV, but not HIV-1, HIV-2, SIV and SIV. The differential reactivity of these monoclonal antibodies indicated that common conserved antigenic epitopes are shared between SIV and SIV with respect to p27 MAbs and between SIV and SIV with respect to p17. Since these MAbs reacted differently with the SIV isolates, they are useful reagents for comparative pathogenesis studies for differentiating SIV isolates.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-1-247
1990-01-01
2019-11-22
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-1-247
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error